Establishment of novel technique using primordial germ cells induced from embryonic stem cells

Research Project

Project/Area Number	24680045
Research Category	Grant-in-Aid for Young Scientists (A)
Allocation Type	Partial Multi-year Fund
Research Field	Laboratory animal science
Research Institution	Kyoto University
Principal Investigator	OHTA Hiroshi 京都大学, 医学(系)研究科(研究院), 助教 (50391892)
Project Period (FY)	2012-04-01 – 2015-03-31
Project Status	Completed (Fiscal Year 2014)
Budget Amount *help	¥26,130,000 (Direct Cost: ¥20,100,000、Indirect Cost: ¥6,030,000) Fiscal Year 2014: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000) Fiscal Year 2013: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000) Fiscal Year 2012: ¥11,570,000 (Direct Cost: ¥8,900,000、Indirect Cost: ¥2,670,000)
Keywords	始原生殖細胞 / ES細胞 / 小分子化合物 / 増殖・分化 / 精子形成 / 卵子形成 / 分化誘導 / 培養 / chemical libray / high-throughput screening
Outline of Final Research Achievements	We have recently succeeded in establishing the culture system to induce primordial germ cell like cells (PGCLCs), which possible to differentiate functional sperm or oocytes, from ES cells or iPS cells. In this study, we performed chemical library screening using PGCLCs and identified the chemicals possible to support the proliferation of PGCLCs for a certain period in vitro. This proliferated PGCLC population in vitro was able to differentiate functional sperm when the cells were trasnplanted into the tetes of infertile male mice. Further improvement of this culture system will allowed us to regulate the proliferation and differentiation of PGCLCs in vitro.