| Budget Amount *help |
¥26,130,000 (Direct Cost: ¥20,100,000、Indirect Cost: ¥6,030,000)
Fiscal Year 2014: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
Fiscal Year 2013: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
Fiscal Year 2012: ¥11,570,000 (Direct Cost: ¥8,900,000、Indirect Cost: ¥2,670,000)
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| Outline of Final Research Achievements |
We have recently succeeded in establishing the culture system to induce primordial germ cell like cells (PGCLCs), which possible to differentiate functional sperm or oocytes, from ES cells or iPS cells. In this study, we performed chemical library screening using PGCLCs and identified the chemicals possible to support the proliferation of PGCLCs for a certain period in vitro. This proliferated PGCLC population in vitro was able to differentiate functional sperm when the cells were trasnplanted into the tetes of infertile male mice. Further improvement of this culture system will allowed us to regulate the proliferation and differentiation of PGCLCs in vitro.
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