Transient/sustained activation of pro-inflammatory signaling that depends on cellular mechanical forces
| Project/Area Number |
24680049
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Partial Multi-year Fund |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Nagoya Institute of Technology (2013-2014)
Tohoku University (2012) |
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Principal Investigator |
DEGUCHI Shinji 名古屋工業大学, 工学(系)研究科(研究院), 准教授 (30379713)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥22,230,000 (Direct Cost: ¥17,100,000、Indirect Cost: ¥5,130,000)
Fiscal Year 2014: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
Fiscal Year 2013: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
Fiscal Year 2012: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
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| Keywords | 張力ホメオスタシス / 適応 / メカノコントローラー / メカノバイオロジー / 非筋II型ミオシン / 焦点接着斑 / 炎症促進反応慢性化 / 細胞生物物理学 / 細胞バイオメカニクス / 力学環境 |
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| Outline of Final Research Achievements |
Inflammation is associated with the immune response in the body, but here we hypothesize that pro-inflammatory signaling within tissue cells is regulated by mechanical forces in a manner independent of the immune response. We particularly focus on a particular protein, paxillin, which constitutes cell–substrate adhesions or focal adhesions where cellular contractile forces are constantly exerted. Tyrosine phosphorylation of paxillin is significantly increased upon reduction in cellular contractile forces, which then activates rac1 and induces pro-inflammatory response. These results show that cells exhibit chronic and transient activation of pro-inflammatory response in the absence and presence of contractile forces, respectively.
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Report
(4 results)
Research Products
(54 results)
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[Presentation] 培養細胞の収縮力の可視化2015
Author(s)
出口真次
Organizer
第9回NIBBバイオイメージングフォーラム
Place of Presentation
岡崎コンファレンスセンター（岡崎市）
Year and Date
2015-01-26 – 2015-01-27
Related Report
Invited
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[Presentation] 細胞収縮力のアッセイ技術2015
Author(s)
出口真次、横山奨、松井翼、加藤輝
Organizer
シンポジウム：細胞アッセイ技術の現状と将来
Place of Presentation
東京大学（東京）
Year and Date
2015-01-13
Related Report
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