Development of in vitro models of extracellular environments and thier applications to cell function study
Project/Area Number |
24680050
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Research Category |
Grant-in-Aid for Young Scientists (A)
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Allocation Type | Partial Multi-year Fund |
Research Field |
Biomedical engineering/Biological material science
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Research Institution | Saitama University |
Principal Investigator |
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Project Period (FY) |
2012-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥27,820,000 (Direct Cost: ¥21,400,000、Indirect Cost: ¥6,420,000)
Fiscal Year 2014: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2013: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
Fiscal Year 2012: ¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000)
|
Keywords | 細胞接着 / 細胞力学 / 光計測 / 高分子基板 / がん細胞 / 細胞組織形成 / ソフトマター / 脂質膜 / 生物物理 / 国際情報交換・ドイツ / バイオテクノロジー / 細胞・組織 / 応用光学・量子光学 / 界面科学 |
Outline of Final Research Achievements |
New experimental platforms for the quantitative evaluation of the influence of extracellular environments on cell functions have been developed. This platform utilizes hydrogel or lipid membrane substrate with tunable mechanical and biochemical cues. It was successfully demonstrated that various cell responses (e.g., adhesion strength and local cell membrane structure) to the substrate as in vitro models of extracellular environments could be evaluated by using our original optical methods. Finally, the systematic measurements by using the experimental platform could provide quantitative insights into the cell-environment mechanical interactions that promote cancer metastasis and tissue formation.
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Report
(4 results)
Research Products
(40 results)
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[Journal Article] Vascularized and Complex Organ Buds from Diverse Tissues via Mesenchymal Cell-Driven Condensation2015
Author(s)
T. Takebe, M. Eomura, E. Yoshizawa, M. Kimura, H. Koike, Y. Ueno, T. Matsuzaki, T. Yamazaki, T. Toyohara, K. Osafune, H. Nakauchi, H. Y. Yoshikawa, H. Taniguchi
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Journal Title
Cell Stem Cell
Volume: 16
Issue: 5
Pages: 556-565
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Reconstruction of Joint Hyaline Cartilage by Autologous Progenitor Cell s Derived from Ear Elastic Cartilage2014
Author(s)
M. Mizuno, S. Kobayashi, T. Takebe, H. Kan, Y. Yabuki, T. Matsuzaki, H. Y. Yoshikawa, S. Nakabayashi, L. J. Ik, J. Maegawa, H. Taniguchi
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Journal Title
Stem Cells
Volume: 32
Issue: 3
Pages: 816-821
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Laser ablation for protein crystal nucleation and seeding2014
Author(s)
H.Y.Yoshikawa, R.Murai, H.Adachi, S.Sugiyama, M.Maruyama, Y.Takahashi, K.Takano, H.Matsumura, T.Inoue, S.Murakami, H.Masuhara, and Y.Mori
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Journal Title
Chemical Society Reviews
Volume: Vol.43, No.7
Issue: 7
Pages: 2147-2158
DOI
Related Report
Peer Reviewed
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[Journal Article] Effect of Gel−Solution Interface on Femtosecond Laser-Induced Nucleation of Protein2013
Author(s)
S.Nakayama, H.Y.Yoshikawa, R.Murai, M.Kurata, M.Maruyama, S.Sugiyama, Y.Aoki, Y.Takahashi, M.Yoshimura, S.Nakabayashi, H.Adachi, H.Matsumura, T.Inoue, K.Takano, S.Murakami, and Y.Mori
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Journal Title
CRYSTAL GROWTH & DESIGN
Volume: Vol.13, Issue 4
Issue: 4
Pages: 1491-1496
DOI
Related Report
Peer Reviewed
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[Journal Article] Effects of a forced solution flow on the step advancement on {110} faces of tetragonal lysozyme crystals : direct visualization of individual steps under a forced solution flow2012
Author(s)
M.Maruyama, H.Kawahara, G.Sazaki, S.Maki, Y.Takahashi, H.Y.Yoshikawa, S.Sugiyama, H.Adachi, K.Takano, H.Matsumura, T.Inoue, S.Murakami, and Y.Mori
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Journal Title
Crystal Growth & Design
Volume: Vol.12, Issue 6
Issue: 6
Pages: 2856-2863
DOI
Related Report
Peer Reviewed
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[Presentation] Evaluation of the impact of stiffness on cancer cell adhesion2014
Author(s)
Takashi Yamazaki, Takahisa Matsuzaki, Yuko Shimokawa, Masami Suganuma, Motomu Tanaka, Seiichiro Nakabayashi, Hiroshi Yoshikawa
Organizer
The 12th Japan-Korea Joint Symposium on Cancer and Ageing Research
Place of Presentation
Saitama Cancer Reseach Center, Saitama
Year and Date
2014-06-19 – 2014-06-21
Related Report
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[Presentation] Green-tea catechin alters the adhesion state in cancer cells, but not in normal cells2013
Author(s)
K. Masuda, E. Kakinuma, R. Sakamoto, K. Ito, K. Iketaki, T. Matsuzaki, H. Yoshikawa, S. Nakabayashi, H. Yamamoto, H. T. Chi, Y. Sato, T. Tanii
Organizer
第5回国際OCHA学術会議 (ICOS 2013)
Place of Presentation
グランシップ、静岡県
Related Report
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[Presentation] Suppressive effect of epigallocatechin gallate on adhesion of metastatic cancer cells studied by reflection interference contrast microscopy measurement of micropatterned cells2013
Author(s)
R. Sakamoto, K. Masuda, E. Kakinuma, K. Ito, K. Iketaki, T. Matsuzaki, H. Yoshikawa, S. Nakabayashi, H. Yamamoto, Y. Sato, T. Tanii
Organizer
第5回国際OCHA学術会議 (ICOS 2013)
Place of Presentation
グランシップ、静岡県
Related Report
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[Presentation] 自己耳介由来軟骨前駆細胞を用いた関節軟骨再構築2013
Author(s)
水野満, 小林眞司, 武部貴則, 鈴木啓, 村田駿介, 矢吹雄一郎, 安村和則, 松崎賢寿, 吉川洋史, 中林誠一郎, 前川二郎, 谷口英樹
Organizer
第12回日本再生医療学会総会
Place of Presentation
横浜・パシフィコ横浜
Related Report
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[Presentation] 細胞外環境モデルの構築と細胞力学応答の評価
Author(s)
山崎喬, 松崎賢寿, 菅沼雅美, Motomu Tanaka, 中林誠一郎, 吉川洋史
Organizer
バイオアセンブラ第2回若手シンポジウム
Place of Presentation
東京大学 生産技術研究所 コンベンションホール、東京都
Related Report
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