| Project/Area Number |
24680052
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Partial Multi-year Fund |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Kyoto University |
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Principal Investigator |
HASEGAWA Kouichi 京都大学, 物質-細胞統合システム拠点, 講師 (50378890)
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| Research Collaborator |
KAHN Michael University of Southern California, 教授
WELLS Christen The University of Queensland, 准教授
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥26,780,000 (Direct Cost: ¥20,600,000、Indirect Cost: ¥6,180,000)
Fiscal Year 2013: ¥12,740,000 (Direct Cost: ¥9,800,000、Indirect Cost: ¥2,940,000)
Fiscal Year 2012: ¥14,040,000 (Direct Cost: ¥10,800,000、Indirect Cost: ¥3,240,000)
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| Keywords | 再生医工学材料 / 化学合成培地 / 生物・生体工学 / 再生医療 / 生体材料 / 組織・細胞 / 再生医学 |
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| Research Abstract |
The extrinsic factors regulating human ES/iPS cell self-renewal are incompletely understood to date, but bFGF and TGFß signaling form the cornerstone of most systems for human ES/iPS cell propagation. In previous study, we have identified a DYRK inhibitor that could modulate Wnt pathway and support Wnt-induced self-renewal without inducing differentiation. Utilizing Wnt and the compound, we have developed a defined xeno-free culture system that allows for long-term expansion of human ES/iPS cells without FGF/TGFß supplementation. In further investigation, we have identified several key pathways involved in DYRK inhibition/Wnt-dependent self-renewal and found small chemical compounds can control these. Utilizing these chemical compounds, we have developed a novel and simple defined-culture system for human ES/iPS cells.
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