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Label-free imaging technique based on nonlinear optics in biological tissues for medical application

Research Project

Project/Area Number 24680060
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypePartial Multi-year Fund
Research Field Medical systems
Research InstitutionEhime University

Principal Investigator

OSHIMA Yusuke  愛媛大学, 医学(系)研究科(研究院), 助教 (10586639)

Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥24,310,000 (Direct Cost: ¥18,700,000、Indirect Cost: ¥5,610,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2013: ¥9,880,000 (Direct Cost: ¥7,600,000、Indirect Cost: ¥2,280,000)
Fiscal Year 2012: ¥12,220,000 (Direct Cost: ¥9,400,000、Indirect Cost: ¥2,820,000)
Keywords無染色イメージング / 第2高調波発生 / ラマン分光 / 非線形光学 / ラマンイメージング / SHGイメージング / 診断 / 非線形光学イメージング / 顕微ラマン分光 / 2光子蛍光 / がん診断 / ライブセルイメージング
Outline of Final Research Achievements

Optical imaging technique enables us to reveal molecular dynamics and their function in cells and tissues. Especially, Raman spectroscopy and nonlinear optical effect are applicable to label-free imaging of target molecules, therefore it is promising tool for biomedical applications. In this study, we developed high throughput Raman imaging system for clinical diagnostics and successfully evaluated nonlinear microscopy for quantitative analysis of changes in molecular component on animal disease models.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • Research Products

    (43 results)

All 2015 2014 2013 2012 Other

All Journal Article (11 results) (of which Peer Reviewed: 10 results,  Open Access: 4 results,  Acknowledgement Compliant: 6 results) Presentation (23 results) (of which Invited: 9 results) Book (8 results) Remarks (1 results)

  • [Journal Article] Quantitative SHG imaging in osteoarthritis model mice, implying a diagnostic application2015

    • Author(s)
      Hiroshi Kiyomatsu, Yusuke Oshima, Takashi Saitou, Tsuyoshi Miyazaki, Atsuhiko Hikita, Hiromasa Miura, Tadahiro Iimura, Takeshi Imamura
    • Journal Title

      Biomedical Optics Express

      Volume: 6 Issue: 2 Pages: 405-420

    • DOI

      10.1364/boe.6.000405

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Changes in chemical composition of bone matrix in ovariectomized (OVX) rats detected by Raman spectroscopy and multivariate analysis2015

    • Author(s)
      Yusuke Oshima, Tadahiro Iimura, Takashi Saitou, Takeshi Imamura
    • Journal Title

      SPIE Proceedings

      Volume: 9303 Pages: 9303-9303

    • DOI

      10.1117/12.2078787

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Ultrasensitive imaging of Ca2+ dynamics in pancreatic acinar cells of Yellow Cameleon-Nano transgenic mice2014

    • Author(s)
      Yusuke Oshima, Takeshi Imamura, Atsuko Shintani, Hiroko Kajiura-Kobayashi, Terumasa Hibi, Takeharu Nagai, Shigenori Nonaka, Tomomi Nemoto
    • Journal Title

      Int. J. Mol. Sci.

      Volume: 15 Issue: 11 Pages: 19971-19986

    • DOI

      10.3390/ijms151119971

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Wide field intravital imaging by two-photonexcitation digital-scanned light-sheet microscopy (2p-DSLM) with a high-pulse energy laser2014

    • Author(s)
      Atsushi Maruyama, Yusuke Oshima*, Hiroko Kajiura-Kobayashi, Shigenori Nonaka, Takeshi Imamura, Kiyoshi Naruse
    • Journal Title

      Biomedical Optics Express

      Volume: 5 Issue: 10 Pages: 3311-3325

    • DOI

      10.1364/boe.5.003311

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] In vivo subcellular imaging of tumors in mouse models using a fluorophore-conjugated anti-CEA antibody in TPEM2014

    • Author(s)
      Shigehiro Koga, Yusuke Oshima, Naoki Honkura, Tadahiro Iimura, Kenji Kameda, Koichi Sato, Motohira Yoshida, Yuji Yamamoto, Yuji Watanabe, Atsuhiko Hikita, and Takeshi Imamura
    • Journal Title

      Cancer Science

      Volume: 105(10) Issue: 10 Pages: 1299-1306

    • DOI

      10.1111/cas.12500

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Intravital multiphoton fluorescence imaging and optical manipulation of spinal cord in mice, using a compact fiber laser system2014

    • Author(s)
      Yusuke Oshima, Hideki Horiuch, Naoki Honkura, Atsuhiko Hikita, Tadanori Ogata, Hiromasa Miura, Takeshi Imamura
    • Journal Title

      Lasers in Surgery and Medicine

      Volume: 46 Issue: 7 Pages: 563-572

    • DOI

      10.1002/lsm.22266

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] In vivo imaging of spinal cord in contusion injury model mice by multi-photon microscopy2014

    • Author(s)
      Y. Oshima, H. Horiuchi, T. Ogata, A. Hikita, H. Miura, and T. Imamura
    • Journal Title

      Proc. SPIE

      Volume: 8947 Pages: 8947-7

    • DOI

      10.1117/12.2041042

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 無染色イメージングの技術開発と医療応用 : 細胞のラマン分光から生体の非線形光学イメージングへの技術展開2013

    • Author(s)
      大嶋佑介
    • Journal Title

      光アライアンス

      Volume: 24 Pages: 6-9

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Light sheet-excited spontaneous Raman imaging of a living fish by optical sectioning in a wide field Raman microscope.2012

    • Author(s)
      Yusuke Oshima, Hidetoshi Sato, Hiroko Kajiura- Kobayashi, Tetsuaki Kimura, Kiyoshi Naruse, Shigenori Nonaka
    • Journal Title

      Optics Express

      Volume: 20 Issue: 15 Pages: 16195-204

    • DOI

      10.1364/oe.20.016195

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] プローブレスイメージング(ラマン分光法, SHG/THG)の診断応用2012

    • Author(s)
      大嶋佑介
    • Journal Title

      病理と臨床

      Volume: 30 Pages: 755-761

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 短時間での広視野ラマンイメージングが可能に2012

    • Author(s)
      野中茂紀, 大嶋佑介
    • Journal Title

      OplusE

      Volume: 34 Pages: 996-996

    • Related Report
      2012 Annual Research Report
  • [Presentation] Development of wide-field of view 2p-excited light-sheet microscopy2015

    • Author(s)
      Yusuke Oshima, Atsushi Maruyama, Shigenori Nonaka, Takeshi Imamura, Kiyoshi Naruse
    • Organizer
      International Symposium on Multi-dimensional Fluorescence Live Imaging of Cellular Functions and Molecular Activities
    • Place of Presentation
      Kyoto International Conference Center
    • Year and Date
      2015-02-26 – 2015-02-28
    • Related Report
      2014 Annual Research Report
  • [Presentation] Changes in chemical composition of bone matrix in ovariectomized (OVX) rats detected by Raman spectroscopy and multivariate analysis2015

    • Author(s)
      Yusuke Oshima, Tadahiro Iimura, Takashi Saitou, Takeshi Imamura
    • Organizer
      Photonics West 2015 (BiOS2015)
    • Place of Presentation
      San Francisco USA
    • Year and Date
      2015-02-07 – 2015-02-12
    • Related Report
      2014 Annual Research Report
  • [Presentation] , “Changes in chemical composition of bone matrix in osteoporosis detected by Raman spectroscopy and multivariate analysis2015

    • Author(s)
      Yusuke Oshima
    • Organizer
      1st Raman Bio-sensing Seminar
    • Place of Presentation
      Kwansei Gakuin University
    • Year and Date
      2015-01-19 – 2015-01-20
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] ラマン分光法による骨粗鬆症ラット骨基質の分子計測2015

    • Author(s)
      大嶋佑介、飯村忠浩、齋藤卓、今村健志
    • Organizer
      第35回レーザー学会学術講演会
    • Place of Presentation
      東海大学高輪校舎
    • Year and Date
      2015-01-11 – 2015-01-12
    • Related Report
      2014 Annual Research Report
  • [Presentation] ラマン分光法および多変量解析による骨粗鬆症ラットの骨基質の解析2014

    • Author(s)
      大嶋佑介、飯村忠浩、齋藤卓、今村健志
    • Organizer
      第37回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2014-11-25 – 2014-11-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] ラマン分光・イメージング技術の開発と骨代謝研究への応用2014

    • Author(s)
      大嶋佑介, 飯村忠浩, 疋田温彦, 今村健志
    • Organizer
      第32回日本骨代謝学会学術集会
    • Place of Presentation
      大阪国際会議場
    • Year and Date
      2014-07-24 – 2014-07-26
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] ラマン分光分析を基盤とした骨質計測技術の開発2014

    • Author(s)
      大嶋佑介, 飯村忠浩, 疋田温彦, 今村健志
    • Organizer
      第34回日本骨形態計測学会シンポジウム
    • Place of Presentation
      さっぽろ芸文館
    • Year and Date
      2014-06-12 – 2014-06-14
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] ラマン分光法を用いた骨代謝・骨形態計測2014

    • Author(s)
      大嶋佑介
    • Organizer
      第4回バイオラマン研究会,最先端光計測とライフサイエンスの近未来 -バイオ・ラマン2017-
    • Place of Presentation
      東北大学片平キャンパス片平さくらホール
    • Year and Date
      2014-05-02
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Raman spectroscopic study for osteoporosis in ovariectomized (OVX) rats2014

    • Author(s)
      Yusuke Oshima, Tadahiro Iimura, Atsuhiko Hikita, Takeshi Imamura
    • Organizer
      Biomedical Imaging and Sensing Conference ’14 (BISC’14)
    • Place of Presentation
      Yokohama
    • Year and Date
      2014-04-22 – 2014-04-24
    • Related Report
      2014 Annual Research Report
  • [Presentation] In vivo imaging of spinal cord in contusion injury model mice by multi-photon microscopy2014

    • Author(s)
      Y. Oshima, H. Horiuchi, T. Ogata, A. Hikita, H. Miura, and T. Imamura
    • Organizer
      Photonics West 2014, Biomedical Optics (BIOS 2014)
    • Place of Presentation
      San Francisco, CA, USA
    • Related Report
      2013 Annual Research Report
  • [Presentation] 種々のバイオイメージング技術の構築について2013

    • Author(s)
      大嶋佑介
    • Organizer
      第11回医用分光学研究会
    • Place of Presentation
      福井県 三国観光ホテル
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Development of nonlinear optical imaging technique for medical applications by the new optical devises2013

    • Author(s)
      Yusuke Oshima, Takashi Katagiri, Yuji Matsuura, Atsuhiko Hikita and Takeshi Imamura
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      兵庫県 ポートピアホテル
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Probe-less optical imaging technique for medical applications based on Raman spectroscopy and nonlinear optics2013

    • Author(s)
      Yusuke Oshima, Atsuhiko. Hikita, Takeshi Imamura
    • Organizer
      TGF-b Family: Signal Network and Live Imaging
    • Place of Presentation
      愛媛県 大和屋本店
    • Related Report
      2013 Annual Research Report
  • [Presentation] Real time monitoring for differentiation of primary osteoblast culture by using Raman microscopy2013

    • Author(s)
      Yusuke Oshima, Atsuhiko Hikita, and Takeshi Imamura
    • Organizer
      ASBMR 2013 Annual Meeting
    • Place of Presentation
      Baltimore, MD, USA
    • Related Report
      2013 Annual Research Report
  • [Presentation] ラマン分光法を用いたがん細胞の無染色イメージング技術の開発2013

    • Author(s)
      大嶋佑介,疋田温彦,今村健志
    • Organizer
      第86回日本生化学会大会
    • Place of Presentation
      神奈川県 パシフィコ横浜
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] In vivoイメージングのための非線形光学顕微鏡の開発と医療応用2013

    • Author(s)
      大嶋佑介,本蔵直樹,疋田温彦,今村健志
    • Organizer
      第39回レーザ顕微鏡研究会講演会
    • Place of Presentation
      埼玉県 理化学研究所
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] ラマン分光法・非線形光学イメージングによる骨基質の分子計測2013

    • Author(s)
      大嶋佑介,疋田温彦,今村健志
    • Organizer
      第33回日本骨形態計測学会
    • Place of Presentation
      静岡県 アクトシティ浜松
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] In Vivo Imaging and Tissue Ablation for Spinal Cord Injury Model Mice Using Multiphoton Microscopy System with 1045 nm Femtosecond Fiber Laser2013

    • Author(s)
      Yusuke Oshima, Hideki Horiuchi, Tadanori Ogata, Atsuhiko Hikita, Hiromasa Miura, and Takeshi Imamura
    • Organizer
      Conference on Laser Surgery and Medicine 2013 (CLSM 2013)
    • Place of Presentation
      Yokohama JAPAN
    • Related Report
      2013 Annual Research Report
  • [Presentation] Development of probe-less imaging techniques for translational research2012

    • Author(s)
      Yusuke Oshima, Naoki Honkura, Atsuhiko Hikita and Takeshi Imamura
    • Organizer
      第35回日本分子生物学会年会ワークショップ
    • Place of Presentation
      福岡マリンメツセ(福岡)(招待講演)
    • Year and Date
      2012-12-12
    • Related Report
      2012 Annual Research Report
  • [Presentation] ハイスループットラマン分光イメージングシステムを用いた生体組織の網羅的解析2012

    • Author(s)
      大嶋佑介, 疋田温彦, 今村健志
    • Organizer
      理化学研究所 第2回バイオラマン研究会, 最先端光計測とライフサイエンスの近未来 : バイオ・ラマン2017
    • Place of Presentation
      理化学研究所(埼玉県)(招待講演)
    • Year and Date
      2012-10-28
    • Related Report
      2012 Annual Research Report
  • [Presentation] 生体の無染色イメージング技術開発と医学応用2012

    • Author(s)
      大嶋佑介, 今村健志
    • Organizer
      2012年・秋季 第73回応用物理学会学術講演会一般, ポスター)
    • Place of Presentation
      愛媛大学(愛媛県)
    • Year and Date
      2012-09-13
    • Related Report
      2012 Annual Research Report
  • [Presentation] ラマン分光法の医学応用 : 生細胞計測, 装置開発から診断に向けて2012

    • Author(s)
      大嶋佑介, 橋本剛佑, 疋田温彦, 小杉浩司, 佐藤英俊, 野中茂紀, 今村健志
    • Organizer
      理化学研究所 第1回バイオラマン研究会, 最先端光計測とライフサイエンスの近未来 : バイオ・ラマン2017.
    • Place of Presentation
      理化学研究所(埼玉県)(招待講演)
    • Year and Date
      2012-05-19
    • Related Report
      2012 Annual Research Report
  • [Presentation] in vivoイメージングのための顕微鏡製作と応用2012

    • Author(s)
      大嶋佑介
    • Organizer
      東京理科大学 第4回イメージングワークショップ
    • Place of Presentation
      東京理科大学(千葉県)(招待講演)
    • Year and Date
      2012-04-04
    • Related Report
      2012 Annual Research Report
  • [Book] The Bone小誌第125号 (2014年4月号)「骨バイオイメージング」ラマン分光法による骨組織の分子計測と骨質評価への応用2014

    • Author(s)
      大嶋佑介,飯村忠浩,疋田温彦,今村健志
    • Total Pages
      6
    • Publisher
      メディカルレビュー社
    • Related Report
      2014 Annual Research Report
  • [Book] バイオイメージングの現状と展望2014

    • Author(s)
      今村健志,疋田温彦,大嶋佑介,飯村忠浩
    • Total Pages
      3
    • Publisher
      インナービジョン
    • Related Report
      2014 Annual Research Report
  • [Book] 「細胞イメージング技術と呼吸器」The Lung perspectives Vol. 22, No. 4 (2014年11月14日発行)「新技術の呼吸器への応用を考える」2014

    • Author(s)
      今村健志、大嶋佑介、岡崎幹生、佐野由文
    • Total Pages
      4
    • Publisher
      メディカルレビュー社
    • Related Report
      2014 Annual Research Report
  • [Book] 「一枚の写真 生体深部を高速かつ広い視野で観察できる光シート顕微鏡」OplusE2014

    • Author(s)
      大嶋佑介
    • Total Pages
      2
    • Publisher
      アドコムメディア株式会社
    • Related Report
      2014 Annual Research Report
  • [Book] 「非線形光学を駆使した生体蛍光癌イメージング」医学のあゆみ2014

    • Author(s)
      今村健志、大嶋佑介、疋田温彦、古賀繁宏
    • Total Pages
      6
    • Publisher
      医歯薬出版
    • Related Report
      2014 Annual Research Report
  • [Book] 「In vivo imagingの最前線」整形・災害外科2013

    • Author(s)
      今村健志,疋田温彦,大嶋佑介
    • Publisher
      金原出版株式会社
    • Related Report
      2013 Annual Research Report
  • [Book] 「骨・軟骨組織の生体光イメージング」月刊誌CLINICAL CALCIUM2013

    • Author(s)
      今村健志、疋田温彦、大嶋佑介、飯村忠浩
    • Publisher
      医薬ジャーナル社
    • Related Report
      2013 Annual Research Report
  • [Book] 先端バイオマテリアルハンドブック 12節 生体組織の分子組成を直接的に計測できる極細径ラマンプローブ2012

    • Author(s)
      佐藤英俊, 大嶋佑介
    • Publisher
      株式会社 エヌ・ティー・エス
    • Related Report
      2012 Annual Research Report
  • [Remarks]

    • URL

      http://www.ehime-u.ac.jp/information/about/publicity/newsletter/detail.html?new_rec=9461

    • Related Report
      2012 Annual Research Report

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Published: 2012-04-24   Modified: 2019-07-29  

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