Comprehensive analysis of pro-inflammatory metabolites in feces for the development of diagnostic biomarkers of metabolic syndrome

• Project/Area Number: 24680075
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Partial Multi-year Fund
• Research Field: Eating habits, studies on eating habits
• Research Institution: University of Shizuoka
• Principal Investigator: MIYOSHI Noriyuki 静岡県立大学, 食品栄養科学部, 助教 (70438191)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥27,430,000 (Direct Cost: ¥21,100,000、Indirect Cost: ¥6,330,000); Fiscal Year 2015: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2014: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2013: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000); Fiscal Year 2012: ¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
• Keywords: 生活習慣病 / 炎症 / 腸管ホメオスターシス / オーミクス解析 / 腸管ホメオスタシス / 健康と食生活

Outline of Final Research Achievements

It is strongly suggested that intestinal microflora is playing pivotal roles in metabolic homeostasis. However some factors, stress, aging, and dietary pattern, possibly influence the intestinal microflora, in which deteriorated composition possibly triggers the low-grade chronic inflammation associated with several lifestyle-related diseases including obesity and diabetic mellitus. In this study, we explored pro-inflammatory molecules in feces from metabolic syndrome model animals. We identified precursor or metabolites of heme were prominently increased in feces of disease group animals including ob/ob, KK-Ay, high-fat fed C57BL/6J mice, and also found that oral administration of the heme metabolites for 8weeks in normal C57BL/6J mice impaired the glucose metabolism. These results suggest that pro-inflammatory metabolites formed in small intestine could be affect to the metabolism of dietary nutrition in host.

Research Products

• [Journal Article] Chemical alterations and regulations of biomolecules in lifestyle-related diseases. (2016)
  • Author(s): Miyoshi N.
  • Journal Title: Biosci. Biotechnol. Biochem. Volume: 1 Issue: 6 Pages: 1-1
  • DOI: 10.1080/09168451.2016.1141037
  • Peer Reviewed
• [Journal Article] Green tea catechins for well-being and therapy: prospects and opportunities. (2015)
  • Author(s): Miyoshi N, Pervin M, Suzuki T, Unno K, Isemura M, Nakamura Y.
  • Journal Title: Botanics: Targets and therapy Volume: 5 Pages: 85-96
  • DOI: 10.2147/btat.s91784
  • Peer Reviewed
• [Journal Article] Comprehensive analysis of the lipophilic reactive carbonyls present in biological specimens by LC/ESI-MS/MS. (2015)
  • Author(s): Susumu Tomono, Noriyuki Miyoshi, Hiroshi Ohshima
  • Journal Title: Journal of Chromatography B Volume: 988 Pages: 149-156
  • DOI: 10.1016/j.jchromb.2015.02.036
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Quercetin-3-O-glucuronide inhibits noradrenaline-promoted invasion of MDA-MB-231 human breast cancer cells by blocking β₂-adrenergic signaling. (2014)
  • Author(s): Yamazaki S, Miyoshi N, Kawabata K, Yasuda M, Shimoi K.
  • Journal Title: Arch Biochem Biophys. Volume: 557 Pages: 18-27
  • DOI: 10.1016/j.abb.2014.05.030
  • Peer Reviewed
• [Journal Article] Anti-inflammatory effects of green soybean extract irradiated with visible light. (2014)
  • Author(s): Tanaka K, Ohgo Y, Katayanagi Y, Yasui K, Hiramoto S, Ikemoto H, Nakata Y, Miyoshi N, Isemura M, Ohashi N, Imai S.
  • Journal Title: Sci Rep. Volume: 4 Issue: 1 Pages: 4732-4732
  • DOI: 10.1038/srep04732
  • Peer Reviewed
• [Journal Article] Implications of cholesterol autoxidation products in the pathogenesis of inflammatory diseases (2014)
  • Author(s): Miyoshi N, Iuliano L, Tomono S, Ohshima H.
  • Journal Title: Biochem. Biophys. Res. Commun. Volume: 446 Issue: 3 Pages: 702-708
  • DOI: 10.1016/j.bbrc.2013.12.107
  • Peer Reviewed
• [Journal Article] Occurrence of cytotoxic 9-oxononanoyl secosterol aldehydes in human low density lipoprotein. (2013)
  • Author(s): Miyoshi N, Iwasaki N, Tomono S, Higashi T, Ohshima H.
  • Journal Title: Free Radic.Biol.Med. Volume: 60 Pages: 73-79
  • DOI: 10.1016/j.freeradbiomed.2013.01.029
  • Peer Reviewed
• [Journal Article] Cytotoxic Effects of Secosterols and Their Derivatives on Several Cultured Cells (2013)
  • Author(s): Tomono S
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 77 Issue: 3 Pages: 651-653
  • DOI: 10.1271/bbb.120758
  • NAID: 10031164790
  • ISSN: 0916-8451, 1347-6947
  • Peer Reviewed
• [Journal Article] Dysregulation of dimethylargininedimethylaminohydrolase/asymmetric dimethylarginine pathway in rat type II diabetic nephropathy (2012)
  • Author(s): Miyoshi N
  • Journal Title: Journal of Clinical Biochemistry and Nutrition Volume: 51 Issue: 2 Pages: 143-149
  • DOI: 10.3164/jcbn.11-33
  • NAID: 130004879298
  • ISSN: 0912-0009, 1880-5086
  • Peer Reviewed
• [Journal Article] Development and application of a method for identification of isothiocyanate-targeted molecules in colon cancer cells (2012)
  • Author(s): Miyoshi, N., Yonemochi, T., Tomono, S., Fukutomi, R., Nakamura, Y., Ohshima, H.
  • Journal Title: Anal. Biochem. Volume: 429(2) Issue: 2 Pages: 124-131
  • DOI: 10.1016/j.ab.2012.07.018
  • Peer Reviewed
• [Journal Article] Health-promoting effects of green tea (2012)
  • Author(s): Suzuki Y., et al.
  • Journal Title: Proceedings of the Japan Academy, Series B Volume: 88 Issue: 3 Pages: 88-101
  • DOI: 10.2183/pjab.88.88
  • NAID: 130001924740
  • ISSN: 0386-2208, 1349-2896
  • Peer Reviewed
• [Journal Article] Effects of a catechin-free fraction derived from green tea on gene expression of enzymes related to lipid metabolism in the mouse liver (2012)
  • Author(s): Yasui K., et al.
  • Journal Title: Biomedical Research Volume: 33 Issue: 1 Pages: 9-13
  • DOI: 10.2220/biomedres.33.9
  • NAID: 130004470805
  • ISSN: 0388-6107, 1880-313X
  • Peer Reviewed
• [Journal Article] α-Tocopherol Sensitizes Human Leukemia HL-60 Cells to Apoptosis Induced by Benzyl Isothiocyanate (2012)
  • Author(s): Abe N., et al
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 76 Issue: 2 Pages: 381-383
  • DOI: 10.1271/bbb.110665
  • NAID: 10030400242
  • ISSN: 0916-8451, 1347-6947
  • Peer Reviewed
• [Journal Article] Analysis of N^ε-ethyllysine in human plasma proteins by gas chromatography-negative ion chemical ionization/mass spectrometry as a biomarker for exposure to acetaldehyde and alcohol (2012)
  • Author(s): Mabuchi R., et al.
  • Journal Title: Alcohol.Clin.Exp.Res. Volume: (印刷中) Issue: 6 Pages: 1013-1020
  • DOI: 10.1111/j.1530-0277.2011.01705.x
  • Peer Reviewed
• [Presentation] Soybean peptide, leginsulin, in different cultivars and its insulin-like activity (2015)
  • Author(s): Noriyuki Miyoshi
  • Organizer: ICoFF 2015
  • Place of Presentation: 岡山
  • Year and Date: 2015-11-22
  • Int'l Joint Research / Invited
• [Presentation] 食品成分と内因性分子による生活習慣病の促進メカニズムと予防に関する生物化学分析 (2015)
  • Author(s): 三好規之
  • Organizer: 日本農芸化学会中部支部第173回例会
  • Place of Presentation: 岡山
  • Year and Date: 2015-06-20
  • Invited
• [Presentation] Lipopolysaccharide構成分子lipid AのLC-MS分析 (2014)
  • Author(s): 大石美月、大島寛史、三好規之
  • Organizer: 第86回日本生化学会
  • Place of Presentation: 京都
  • Year and Date: 2014-10-15 – 2014-10-18
• [Presentation] ob/obマウス糞便に含まれる起炎性分子の探索 (2014)
  • Author(s): 眞田峻佑、大島寛史、三好規之
  • Organizer: 日本農芸化学会2014
  • Place of Presentation: 東京
• [Presentation] 食後の血中脂質に対するヤムイモおよびその含有成分ジオスゲニンの効果 (2013)
  • Author(s): 三好規之
  • Organizer: 日本農芸化学会2013
  • Place of Presentation: 仙台
• [Presentation] オゾン酸化コレステロール : 生成機構、生理活性および生活習慣病発症における役割 (2012)
  • Author(s): 三好規之
  • Organizer: 第65回日本酸化ストレス学会
  • Place of Presentation: 徳島
• [Presentation] 肥満モデルマウスKK-Ayにおける山薬およびジオスゲニンの大腸発がん予防分子機構解析 (2012)
  • Author(s): 三好規之
  • Organizer: がん予防学会2012
  • Place of Presentation: 岐阜
• [Presentation] 糖尿病モデルラット肝におけるDNA付加体の網羅的解析 (2012)
  • Author(s): 三好規之
  • Organizer: 第71回日本癌学会
  • Place of Presentation: 札幌
• [Presentation] がん予防食品因子イソチオシアネート結合タンパク質の網羅的探索法 (2012)
  • Author(s): 三好規之
  • Organizer: 第84回日本生化学会
  • Place of Presentation: 博多