Block of proliferation and survival of colon cancer cells by intestine-specific homeoprotein CDX2
Project/Area Number |
24680091
|
Research Category |
Grant-in-Aid for Young Scientists (A)
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Allocation Type | Partial Multi-year Fund |
Research Field |
Tumor biology
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Research Institution | University of Fukui |
Principal Investigator |
Aoki Koji 福井大学, 学術研究院医学系部門, 教授 (40402862)
|
Project Period (FY) |
2012-04-01 – 2017-03-31
|
Project Status |
Completed (Fiscal Year 2016)
|
Budget Amount *help |
¥26,780,000 (Direct Cost: ¥20,600,000、Indirect Cost: ¥6,180,000)
Fiscal Year 2015: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2014: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2013: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2012: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
|
Keywords | CDX2 / 大腸癌 / オートファジー / ATG7 / ROS / 細胞生存 / 活性酸素 / 増殖 / 細胞増殖 / 細胞死 / 細胞周期 |
Outline of Final Research Achievements |
In this study, we have found that intestine-specific homeoprotein CDX2 bound to ATG7, an essential enzyme for autophagy, directly via its homeodomain and that CDX2 stimulated autophagy in colon cancer cells. We have also found that CDX2 strongly suppressed proliferation and survival of colon cancer cells through autophagy activation. Notably, inhibition of cancer cell proliferation and survival by CDX2 were mediated by increased levels of reactive oxygen species (ROS) caused through autophagy activation. Interestingly, CDX2 increased levels o ROS and suppressed cancer cell survival upon its detachment more strongly than attachment. These results have suggested that CDX2 blocked malignant progression of colon cancer cells including metastasis as well as tumor development.
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Report
(6 results)
Research Products
(14 results)
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[Journal Article] Mitochondrial function provides instructive signals for activation-induced B cell fates2015
Author(s)
Kyoung-Jin Jang, Hiroto Mano, Koji Aoki, Tatsunari Hayashi, Yukiko Nambu, Eri Hara, Katsu Takahashi, Katsuhiko Itoh, Shigeru Taketani, Kazuhiko Igarashi, Akira Shimizu and Manabu Sugai
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Journal Title
Nature Commun
Volume: 6
Issue: 1
Pages: 6750-6750
DOI
NAID
Related Report
Peer Reviewed / Open Access
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[Journal Article] Reducing DNA methylation suppresses colon carcinogenesis by inducing tumor cell differentiation. Carcinogenesis.2015
Author(s)
Hatano Y, Semi K, Hashimoto K, Lee MS, Hirata A, Tomita H, Kuno T, Takamatsu M, Aoki K, Taketo MM, Kim YJ, Hara A, Yamada Y.
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Journal Title
Carcinogenesis
Volume: 36
Issue: 7
Pages: 719-29
DOI
Related Report
Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] In situ differentiation of CD8αα T cells from CD4 T cells in peripheral lymphoid tissues2012
Author(s)
Nambu Y., Hayashi T., Jang KJ, Aoki K., Mano H., Nakano K., Osato M., Takabashi K., Itoh K., Teramukai S., Komon T., Fujita J., Ito Y., Shimizu
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Journal Title
Scientific Reports
Volume: 2
Issue: 1
Pages: 930-930
DOI
Related Report
Peer Reviewed
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