| Budget Amount *help |
¥26,780,000 (Direct Cost: ¥20,600,000、Indirect Cost: ¥6,180,000)
Fiscal Year 2015: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2014: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2013: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2012: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
|
|---|
| Outline of Final Research Achievements |
In this study, we have found that intestine-specific homeoprotein CDX2 bound to ATG7, an essential enzyme for autophagy, directly via its homeodomain and that CDX2 stimulated autophagy in colon cancer cells. We have also found that CDX2 strongly suppressed proliferation and survival of colon cancer cells through autophagy activation. Notably, inhibition of cancer cell proliferation and survival by CDX2 were mediated by increased levels of reactive oxygen species (ROS) caused through autophagy activation. Interestingly, CDX2 increased levels o ROS and suppressed cancer cell survival upon its detachment more strongly than attachment. These results have suggested that CDX2 blocked malignant progression of colon cancer cells including metastasis as well as tumor development.
|
