Identification of long non-coding RNAs critically involved in colon cancer
| Project/Area Number |
24680092
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Partial Multi-year Fund |
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| Research Field |
Tumor diagnosis
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| Research Institution | Sapporo Medical University |
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Principal Investigator |
MARUYAMA Reo 札幌医科大学, 医学部, 准教授 (60607985)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥26,910,000 (Direct Cost: ¥20,700,000、Indirect Cost: ¥6,210,000)
Fiscal Year 2014: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000)
Fiscal Year 2013: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
Fiscal Year 2012: ¥9,620,000 (Direct Cost: ¥7,400,000、Indirect Cost: ¥2,220,000)
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| Keywords | lncRNA / 長鎖nRNA / 大腸癌 / ヒストン修飾 / DNAメチル化 / 長鎖ncRNA / ChIPSeq / バイオインフォマティクス |
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| Outline of Final Research Achievements |
There is growing evidence that long non-coding RNAs (lncRNAs) play important roles in a wide repertoire of biological processes. It is very likely that lncRNAs also play critical roles in colorectal cancer (CRC). However, few studies have reported the involvement of lncRNAs in CRC. In this study, we aimed to comprehensively identify novel lncRNAs involved in CRC. To search for novel lncRNAs, we used ChIP-seq technology to obtain histone methylation profiles in endoscopic biopsy specimens. We characterized global histone modification plofiles in more than 20 normal tissues and cancers. We then examined histone status around putative lncRNAs and generated a list of candidate lncRNAs. In order to narrow the list down, we also utilized publicly available DNA methylation data as well as RNASeq to perform integrated analysis. Finally we identified ~10 lncRNAs silenced by DNA methylation and also identified ~10 lncRNAs that are abnormally overexpressed in CRC.
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Report
(4 results)
Research Products
(14 results)
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[Journal Article] Analysis of DNA methylation in bowel lavage fluid for detection of colorectal cancer2014
Author(s)
Harada T, Yamamoto E, Yamano HO, Nojima M, Maruyama R, Kumegawa K, Ashida M, Yoshikawa K, Kimura T, Harada E, Takagi R, Tanaka Y, Aoki H, Nishizono M, Nakaoka M, Tsuyada A, Niinuma T, Kai M, Shimoda K, Shinomura Y, Sugai T, Imai K, Suzuki H
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Journal Title
Cancer prevention research
Volume: 7
Pages: 1002-1010
Related Report
Peer Reviewed
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[Presentation] Systematic identification of long non-coding RNAs involved in gastritis and gastric tumorigenesis.2014
Author(s)
Maruyama R, Kitajima H, Yamamoto E, Niinuma T, Yorozu A, Kumegawa K, Nishiyama K, Tsuyada A, Suzuki R, Kai M, Tokino T, Shinomura Y, Suzuki H.
Organizer
第37回日本分子生物学会年会
Place of Presentation
横浜
Year and Date
2014-11-25 – 2014-11-27
Related Report
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[Presentation] Systematic identification of long non-coding RNAs involved in gastritis and gastric tumorigenesis.2014
Author(s)
Maruyama R, Kitajima H, Yamamoto E, Niinuma T, Yorozu A, Kumegawa K, Tsuyada A, Suzuki R, Kai M, Tokino T, Shinomura Y, Suzuki H.
Organizer
第73回日本癌学会学術総会
Place of Presentation
横浜
Year and Date
2014-09-25 – 2014-09-27
Related Report
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[Presentation] Genome-wide identification of lincRNAs epigenetically silenced by DNA methylation in colon cancer2014
Author(s)
Igarashi T, Kumegawa K, Maruyama R, Yamamoto E, Tsuyada A, Suzuki R, Niinuma T, Kai M, Yamano H, Sugai T, Tokino T, Shinomura Y, Suzuki H.
Organizer
第73回日本癌学会学術総会
Place of Presentation
横浜
Year and Date
2014-09-25 – 2014-09-27
Related Report
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