| Budget Amount *help |
¥26,650,000 (Direct Cost: ¥20,500,000、Indirect Cost: ¥6,150,000)
Fiscal Year 2014: ¥9,230,000 (Direct Cost: ¥7,100,000、Indirect Cost: ¥2,130,000)
Fiscal Year 2013: ¥9,230,000 (Direct Cost: ¥7,100,000、Indirect Cost: ¥2,130,000)
Fiscal Year 2012: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
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| Outline of Final Research Achievements |
In order to gain broader insight into the phosphorylation signal controlling DNA damage response (DDR), system-wide analysis of phosphorylation dynamics after gamma irradiation has been investigated. 25971 phosphosites were identified with accurate quantification. 6555 phosphosites displayed dynamic changes in phosphorylation status during one hour after irradiation. We identified distinct dynamic phosphorylation patterns of kinases, which had not been known to be associated with DNA damage, by the bioinformatics analysis based on matching sequence motif and protein-protein interaction. We found that DNA damage signaling is activated and colony formation is inhibited by the inhibition of the kinases.
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