High-sensitive proteomic analysis of proteins modified in response to DNA damage.
| Project/Area Number |
24681009
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Partial Multi-year Fund |
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| Research Field |
Risk sciences of radiation/Chemicals
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| Research Institution | 独立行政法人医薬基盤研究所 |
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Principal Investigator |
ADACHI Jun 独立行政法人医薬基盤研究所, その他部局等, 研究員 (20437255)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥26,650,000 (Direct Cost: ¥20,500,000、Indirect Cost: ¥6,150,000)
Fiscal Year 2014: ¥9,230,000 (Direct Cost: ¥7,100,000、Indirect Cost: ¥2,130,000)
Fiscal Year 2013: ¥9,230,000 (Direct Cost: ¥7,100,000、Indirect Cost: ¥2,130,000)
Fiscal Year 2012: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
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| Keywords | DNA損傷応答 / シグナル伝達 / 翻訳後修飾 / プロテオミクス / 質量分析 / プロテオーム / リン酸化 / ユビキチン化 |
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| Outline of Final Research Achievements |
In order to gain broader insight into the phosphorylation signal controlling DNA damage response (DDR), system-wide analysis of phosphorylation dynamics after gamma irradiation has been investigated. 25971 phosphosites were identified with accurate quantification. 6555 phosphosites displayed dynamic changes in phosphorylation status during one hour after irradiation. We identified distinct dynamic phosphorylation patterns of kinases, which had not been known to be associated with DNA damage, by the bioinformatics analysis based on matching sequence motif and protein-protein interaction. We found that DNA damage signaling is activated and colony formation is inhibited by the inhibition of the kinases.
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Report
(4 results)
Research Products
(13 results)
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[Journal Article] A novel mechanism of keratin cytoskeleton organization through casein kinase I alpha and FAM83H in colorectal cancer.2013
Author(s)
Kuga, T., Kume, H., Kawasaki, N., Sato, M., Adachi, J., Shiromizu, T., Hoshino, I., Nishimori, T., Matsubara, H., and Tomonaga T.
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Journal Title
J Cell Sci
Volume: 126
Pages: 4721-31
DOI
Related Report
Peer Reviewed
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