Elucidation of PHA accumulation mechanism by mixed microbial cultures with high PHA accumulation ability from wastewater substrates

• Project/Area Number: 24681010
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Partial Multi-year Fund
• Research Field: Environmental technology/Environmental materials
• Research Institution: Kitasato University
• Principal Investigator: INOUE Daisuke 北里大学, 医療衛生学部, 准教授 (70448091)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥24,830,000 (Direct Cost: ¥19,100,000、Indirect Cost: ¥5,730,000); Fiscal Year 2014: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000); Fiscal Year 2013: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000); Fiscal Year 2012: ¥12,870,000 (Direct Cost: ¥9,900,000、Indirect Cost: ¥2,970,000)
• Keywords: 省資源技術 / バイオリファイナリー / PHA / 下水汚泥 / 微生物挙動解析 / ﾊﾞｲｵﾘﾌｧｲﾅﾘｰ / ポリヒドロキシアルカン酸 / 下水基質 / feast-famine / phaC遺伝子

Outline of Final Research Achievements

This study aimed to elucidate the PHA accumulation mechanism from wastewater substrates by mixed microbial cultures with high PHA accumulation ability that are constructed from activated sludge by a feast-famine method. Results of this study revealed the followings: (1) Most of activated sludge bacteria are capable of producing PHA, (2) short-chain fatty acids are preferable substrates for PHA production by PHA-accumulating bacteria in activated sludge, and (3) PHA-accumulating bacteria belonging to Rhodocyclales make a great contribution to PHA accumulation by mixed microbial cultures constructed from activated sludge by a feast-famine method.

Research Products

• [Journal Article] Polyhydroxyalkanoate (PHA) accumulation potential and PHA-accumulating microbial communities in various activated sludge processes of municipal wastewater treatment plants (2015)
  • Author(s): Kousuke Sakai, Sawa Miyake, Koichi Iwama, Daisuke Inoue, Satoshi Soda, Michihiko Ike
  • Journal Title: Journal of Applied Microbiology Volume: 118 Issue: 1 Pages: 255-266
  • DOI: 10.1111/jam.12683
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Polyhydroxyalkanoate production potential of heterotrophic bacteria in activated sludge (2015)
  • Author(s): Daisuke Inoue, Yuta Suzuki, Takahiro Uchida, Jota Morohoshi, Kazunari Sei
  • Journal Title: Journal of Bioscience and Bioengineering Volume: in press
  • NAID: 110010041302
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Bacterial community dynamics in a full-scale municipal wastewater treatment plant employing conventional activated sludge process (2014)
  • Author(s): Kurumi Hashimoto, Masami Matsuda, Daisuke Inoue, Michihiko Ike
  • Journal Title: Journal of Bioscience and Bioengineering Volume: - Issue: 1 Pages: 64-71
  • DOI: 10.1016/j.jbiosc.2013.12.008
  • NAID: 110009838771
  • Peer Reviewed
• [Presentation] Feast-famine法による活性汚泥からのポリヒドロキシアルカン酸蓄積微生物の集積に及ぼすpHの影響 (2015)
  • Author(s): 井上大介, 鈴木悠太, 清和成
  • Organizer: 第49回日本水環境学会年会
  • Place of Presentation: 石川県金沢市（金沢大学）
  • Year and Date: 2015-03-16 – 2015-03-18
• [Presentation] 異なる温度条件下における活性汚泥からのポリヒドロキシアルカン酸蓄積菌の集積 (2014)
  • Author(s): 井上大介, 鈴木悠太, 諸星丈太, 清和成
  • Organizer: 日本水処理生物学会第51回大会
  • Place of Presentation: 山梨県甲府市（山梨県JA会館）
  • Year and Date: 2014-11-12 – 2014-11-14
• [Presentation] Polyhydroxyalkanoate production potential of activated sludge bacteria (2014)
  • Author(s): Daisuke Inoue, Yuta Suzuki, Takahiro Uchida, Jota Morohoshi, Kazunari Sei
  • Organizer: IWA World Water Congress and Exhibition 2014
  • Place of Presentation: Lisbon, Portugal（Lisbon Congress Centre）
  • Year and Date: 2014-09-21 – 2014-09-26
• [Presentation] Effect of temperature on enrichment of polyhydroxyalkanoate accumulating organisms from activated sludge (2014)
  • Author(s): Yuta Suzuki, Jota Morohoshi, Daisuke Inoue, Kazunari Sei
  • Organizer: 7th Advanced Engineering Technology for Environment and Energy –Environment, Energy and Sustainable Development
  • Place of Presentation: Pusan, 韓国（Busan National University）
  • Year and Date: 2014-07-27 – 2014-07-29
• [Presentation] Effect of temperature on enrichment of polyhydroxyalkanoate accumulating organisms in sequencing batch reactors fed with acetate (2014)
  • Author(s): Daisuke Inoue, Yuta Suzuki, Jota Morohoshi, Kazunari Sei
  • Organizer: Water and Environment Technology Conference 2014 (WET2014)
  • Place of Presentation: 東京都新宿区（早稲田大学先端生命医科学センターTWIns）
  • Year and Date: 2014-06-28 – 2014-06-29
• [Presentation] 都市下水処理場の活性汚泥のPHA蓄積能とPHA蓄積微生物群集に関する調査 (2013)
  • Author(s): 酒井孝輔、岩間航一、三宅佐和、惣田訓、池道彦、井上大介
  • Organizer: 第47回日本水環境学会年会
  • Place of Presentation: 大阪工業大学大宮キャンパス (大阪府)
  • Year and Date: 2013-03-13
• [Presentation] Polyhydroxyalkanoate-producing potential of cultivable bacteria in a lab-scale activated sludge reactor (2013)
  • Author(s): Daisuke Inoue, Yuta Suzuki, Kazunari Sei
  • Organizer: Water and Environment Technology Conference 2013 (WET2013)
  • Place of Presentation: 東京農工大学小金井キャンパス（東京都小金井市）
• [Presentation] 都市下水処理場の活性汚泥のPHA蓄積能とPHA蓄積微生物群集 (2013)
  • Author(s): 酒井孝輔, 岩間航一, 三宅佐和, 惣田訓, 池道彦, 井上大介
  • Organizer: 第50回下水道研究発表会
  • Place of Presentation: 東京ビッグサイト（東京都江東区）
• [Presentation] 活性汚泥細菌のポリヒドロキシアルカン酸(PHA)合成ポテンシャルの評価 (2013)
  • Author(s): 鈴木悠太, 井上大介, 清和成
  • Organizer: 日本水処理生物学会第50回大会
  • Place of Presentation: 神戸市水道局たちばな職員研修センター（兵庫県神戸市）
• [Presentation] 活性汚泥中の従属栄養細菌のポリヒドロキシアルカン酸合成ポテンシャルの評価 (2013)
  • Author(s): 井上大介, 鈴木悠太, 清和成
  • Organizer: 第50回環境工学研究フォーラム
  • Place of Presentation: 北海道大学学術交流会館（北海道札幌市）
• [Presentation] 都市下水処理場の活性汚泥中におけるPHA蓄積微生物群集の解析 (2012)
  • Author(s): 酒井孝輔、三宅佐和、井上大介、惣田訓、池道彦
  • Organizer: 第49回環境工学研究フォーラム
  • Place of Presentation: 京都大学百周年時計台記念館 (京都府)
  • Year and Date: 2012-11-29
• [Presentation] 様々な下水処理場の活性汚泥のポリヒドロキシアルカン酸蓄積能に関する調査 (2012)
  • Author(s): 三宅佐和、岩間航一、酒井孝輔、井上大介、惣田訓、池道彦
  • Organizer: 日本水処理生物学会第49回大会
  • Place of Presentation: 北里大学白金キャンパス (東京都)
  • Year and Date: 2012-11-24