| Budget Amount *help |
¥26,520,000 (Direct Cost: ¥20,400,000、Indirect Cost: ¥6,120,000)
Fiscal Year 2014: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥13,000,000 (Direct Cost: ¥10,000,000、Indirect Cost: ¥3,000,000)
Fiscal Year 2012: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
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| Outline of Final Research Achievements |
To identify glycolipid-synthesizing genes, draft genome sequencing was carried out for a glycolipid producing fungi, Pseudozyma hubeiensis SY62. Seventy four scaffolds were obtained from approximately 60,000,000 read of raw data. The total length of scaffolds reached to approximately 18 Mb. MEL synthetic genes were identified by comparing of genome sequences between SY62 and Ustilago maydis 521. Then, transformation method was developed using a vector including green fluorescent protein constructed from Ustilago-Escherichia coli shuttle vector, pUXV1.
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