| Budget Amount *help |
¥26,260,000 (Direct Cost: ¥20,200,000、Indirect Cost: ¥6,060,000)
Fiscal Year 2015: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2014: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2013: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
Fiscal Year 2012: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
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| Outline of Final Research Achievements |
A method of size-selective cell separation using hemispherical superparamagnetic particles, "magcups", was developed. Polystyrene spheres were used for templates of the fabrication, magnetic elements were deposited on the spheres by thermal evaporation, the spheres were removed by burning and the hemispherical superparamagnetic metal shells, magcups, were obtained. Target cells were mixed with a set of different sizes of magcups, then, target cells were only captured in magcups having larger diameters than the cells. The collected cells were grown in culture medium without any damage. These results indicate that the developed method can achieve quick, simple and non-invasive target cell separations depending on their sizes.
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