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Practical application of multi-layered metal nano-cups for studies in life sciences

Research Project

Project/Area Number 24681025
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypePartial Multi-year Fund
Research Field Nanomaterials/Nanobioscience
Research InstitutionKanagawa Academy of Science and Technology

Principal Investigator

Kim Hyonchol  公益財団法人神奈川科学技術アカデミー, オンチップ・セロミクスプロジェクト, 研究員 (70514107)

Project Period (FY) 2012-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥26,260,000 (Direct Cost: ¥20,200,000、Indirect Cost: ¥6,060,000)
Fiscal Year 2015: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2014: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2013: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
Fiscal Year 2012: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
Keywordsナノ機能材料 / 磁性構造体 / 細胞回収技術 / 細胞精製
Outline of Final Research Achievements

A method of size-selective cell separation using hemispherical superparamagnetic particles, "magcups", was developed. Polystyrene spheres were used for templates of the fabrication, magnetic elements were deposited on the spheres by thermal evaporation, the spheres were removed by burning and the hemispherical superparamagnetic metal shells, magcups, were obtained. Target cells were mixed with a set of different sizes of magcups, then, target cells were only captured in magcups having larger diameters than the cells. The collected cells were grown in culture medium without any damage. These results indicate that the developed method can achieve quick, simple and non-invasive target cell separations depending on their sizes.

Report

(5 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • Research Products

    (21 results)

All 2016 2015 2014 2013 2012 Other

All Journal Article (4 results) (of which Peer Reviewed: 4 results,  Open Access: 1 results,  Acknowledgement Compliant: 2 results) Presentation (15 results) (of which Int'l Joint Research: 2 results,  Invited: 1 results) Patent(Industrial Property Rights) (2 results) (of which Overseas: 1 results)

  • [Journal Article] Depletion Effect on Concave Microstructure upon Size-Specific Target Particle Collection2015

    • Author(s)
      Hyonchol Kim, Hideyuki Terazono, Hiroyuki Takei, Kenji Yasuda
    • Journal Title

      Japanese Journal of Applied Physics

      Volume: 未定

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Cup-Shaped Superparamagnetic Hemispheres for Size-Selective Cell Filtration2014

    • Author(s)
      Hyonchol Kim, Hideyuki Terazono, Hiroyuki Takei, Kenji Yasuda
    • Journal Title

      Scientific Reports

      Volume: 4 Issue: 1 Pages: 6362-6362

    • DOI

      10.1038/srep06362

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] DNA Hybridization Efficiency on Concave Surface Nano-Structure in Hemispherical Janus Nanocups2014

    • Author(s)
      Hyonchol Kim, Hideyuki Terazono, Hiroyuki Takei, Kenji Yasuda
    • Journal Title

      Langmuir

      Volume: 30 Issue: 5 Pages: 1272-1280

    • DOI

      10.1021/la403557g

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Fabrication of Multilayered Superparamagnetic Particles Based on Sequential Thermal Deposition Method2014

    • Author(s)
      Hyonchol Kim, Hideyuki Terazono, Hiroyuki Takei, Kenji Yasuda
    • Journal Title

      Japanese Journal of Applied Physics

      Volume: 印刷中

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Presentation] Contribution of Depletion Effect for Size-Selective Target Cell Acquisition in Cup-Shaped Microstructures2016

    • Author(s)
      金 賢徹、寺薗 英之、竹井 弘之、安田 賢二
    • Organizer
      60th American Biophysical Society Annual Meeting
    • Place of Presentation
      Los Angeles Convention Center(アメリカ)
    • Year and Date
      2016-02-28
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Contribution of depletion effect to size-specific target cell purification using mirometer-sized concave structures2015

    • Author(s)
      金 賢徹、寺薗 英之、竹井 弘之、服部 明弘、松浦 賢志、野村 典正、安田 賢二
    • Organizer
      The 53th Annual Meeting of the Biophysical Society of Japan
    • Place of Presentation
      金沢大学(石川県)
    • Year and Date
      2015-09-14
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Size-Specific Target Cell Purification Exploiting Depletion Effect on Cup-Shaped Superparamagnetic Hemispheres2014

    • Author(s)
      金 賢徹、竹井 弘之、寺薗 英之、安田 賢二
    • Organizer
      27th International Microprocesses and Nanotechnology Conference (MNC2014)
    • Place of Presentation
      ヒルトン福岡市ーホーク(福岡県)
    • Year and Date
      2014-11-07
    • Related Report
      2014 Annual Research Report
  • [Presentation] 超常磁性カップの作製とサイズ選択的細胞回収2014

    • Author(s)
      金 賢徹、竹井 弘之、寺薗 英之、安田 賢二
    • Organizer
      第75回応用物理学会学術講演会
    • Place of Presentation
      北海道大学(北海道)
    • Year and Date
      2014-09-19
    • Related Report
      2014 Annual Research Report
  • [Presentation] Development of "Adaptive SEM" Technology for in situ Genome/Proteome Expression Analysis in Single Cell Level2014

    • Author(s)
      金 賢徹、竹井 弘之、寺薗 英之、安田 賢二
    • Organizer
      International Microscopy Congress 2014
    • Place of Presentation
      Prague Congress Centre(チェコ)
    • Year and Date
      2014-09-10 – 2014-09-11
    • Related Report
      2014 Annual Research Report
  • [Presentation] Development of Adaptive SEM Technology for Genome/Proteome Expression Analysis in Single Cell Level2013

    • Author(s)
      金賢徹、寺薗英之、竹井弘之、安田賢二
    • Organizer
      American Biophysical Society 57th Annual Meeting
    • Place of Presentation
      ペンシルバニアコンベンションセンター(米国)
    • Year and Date
      2013-02-06
    • Related Report
      2012 Annual Research Report
  • [Presentation] Fabrication of Superparamagnetic Nano-Particles Having Various Diameters by Strict Controlling of Magnetic Material Thickness2012

    • Author(s)
      金賢徹、寺薗英之、竹井弘之、安田賢二
    • Organizer
      25th International Microprocesses and Nanotechnology Conference (MNC2012)
    • Place of Presentation
      神戸メリケンパークオリエンタルホテル(兵庫県)
    • Year and Date
      2012-11-01
    • Related Report
      2012 Annual Research Report
  • [Presentation] F abrication of Superparamagnetic Janus Particles Having Various Sizes and Its Application for Non-Destructive Cell Sorting2012

    • Author(s)
      金賢徹、寺薗英之、竹井弘之、安田賢二
    • Organizer
      第50回日本生物物理学会年会
    • Place of Presentation
      名古屋大学(愛知県)
    • Year and Date
      2012-09-24
    • Related Report
      2012 Annual Research Report
  • [Presentation] 様々な粒径・材質のヤヌス粒子に対する機能付加法の検討2012

    • Author(s)
      金賢徹、竹井弘之、寺薗英之、安田賢二
    • Organizer
      第73回応用物理学会学術講演会
    • Place of Presentation
      愛媛大学・松山大学(愛媛県)
    • Year and Date
      2012-09-13
    • Related Report
      2012 Annual Research Report
  • [Presentation] 1細胞in situゲノムプロテオーム計測のためのFE-SEM定量反射電子分析法の開発2012

    • Author(s)
      金賢徹、竹井弘之、寺薗英之、安田賢二
    • Organizer
      日本顕微鏡学会第68回学術講演会
    • Place of Presentation
      つくば国際会議場(茨城県)
    • Year and Date
      2012-05-14
    • Related Report
      2012 Annual Research Report
  • [Presentation] 金ナノ粒子標識とFE-SEM計数計測を組み合わせた生体分子高感度検出法の開発

    • Author(s)
      金 賢徹、竹井 弘之、寺薗 英之、安田 賢二
    • Organizer
      日本顕微鏡学会第69回学術講演会
    • Place of Presentation
      ホテル阪急エキスポパーク(大阪府)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Fabrication of Superparamagnetic Metal Cups for Size-Selective Cell Collection

    • Author(s)
      金 賢徹、寺薗 英之、竹井 弘之、安田 賢二
    • Organizer
      第51回日本生物物理学会年会
    • Place of Presentation
      京都国際会館(京都府)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Fabrication of Cup-Shaped Superparamagnetic Metal Hemispheres for Size-Selective Target Cell Collection

    • Author(s)
      金 賢徹、寺薗 英之、竹井 弘之、安田 賢二
    • Organizer
      26th International Microprocesses and Nanotechnology Conference (MNC2013)
    • Place of Presentation
      ロイトン札幌(北海道)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Nano-Cups : Fabrication and Application of Cup-Shaped Functional Nano-Particles forCell Biology

    • Author(s)
      金 賢徹、寺薗 英之、竹井 弘之、安田 賢二
    • Organizer
      1st International Symposium on Nanoparticles/Nanomaterials and Applications
    • Place of Presentation
      Hotel Costa da Caparica(ポルトガル)
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Application of Cup-Shaped Superparamagnetic Hemispheres for Size- Selective Cell Purification

    • Author(s)
      金 賢徹、寺薗 英之、竹井 弘之、安田 賢二
    • Organizer
      American Biophysical Society 58th Annual Meeting
    • Place of Presentation
      San Francisco Moscone Center(アメリカ)
    • Related Report
      2013 Annual Research Report
  • [Patent(Industrial Property Rights)] 磁気ナノ粒子2012

    • Inventor(s)
      安田賢二、金賢徹、寺薗英之、服部明弘
    • Industrial Property Rights Holder
      公益財団法人神奈川科学技術アカデミー、株式会社オンチップ・セロミクス・コンソーシアム、東京医科歯科大学
    • Filing Date
      2012-11-08
    • Related Report
      2012 Annual Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] 微小体の配列方法2012

    • Inventor(s)
      安田賢二、金賢徹、寺薗英之、服部明弘
    • Industrial Property Rights Holder
      公益財団法人神奈川科学技術アカデミー、株式会社オンチップ・セロミクス・コンソーシアム、東京医科歯科大学
    • Industrial Property Number
      2012-272009
    • Filing Date
      2012-12-13
    • Related Report
      2012 Annual Research Report

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Published: 2012-04-24   Modified: 2019-07-29  

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