A single mRNA counting by a microchamber array device containing a single human nucleus
| Project/Area Number |
24681027
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Partial Multi-year Fund |
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| Research Field |
Microdevices/Nanodevices
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| Research Institution | Nagoya University |
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Principal Investigator |
KAJI Noritada 名古屋大学, 工学(系)研究科(研究院), 准教授 (90402479)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥27,170,000 (Direct Cost: ¥20,900,000、Indirect Cost: ¥6,270,000)
Fiscal Year 2014: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2013: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2012: ¥15,860,000 (Direct Cost: ¥12,200,000、Indirect Cost: ¥3,660,000)
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| Keywords | ナノバイオ / mRNA / 細胞 |
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| Outline of Final Research Achievements |
In this research, a microchamber array device was designed to capture a single cell, lyse a cell membrane, and isolate a single cell nucleus, and then mRNA in a single cell nucleus were directly quantitated by using highly sensitive In-Stem Molecular Beacons (ISMB). As a result, the expression of three frequently used house-keeping genes were successfully quantitated to an accuracy of 100 molecules within a single cell nucleus.
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Report
(4 results)
Research Products
(39 results)
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[Journal Article] Self-assembled Nanowire Arrays as Three-dimensional Nanopores for Filtration of DNA Molecules2015
Author(s)
S. Rahong, T. Yasui, T. Yanagida, K. Nagashima, M. Kanai, G. Meng, Y. He, F. Zhuge, N. Kaji, T. Kawai, Y. Baba
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Journal Title
Analytical Sciences
Volume: 31
Issue: 3
Pages: 153-157
DOI
NAID
ISSN
0910-6340, 1348-2246
Related Report
Peer Reviewed / Open Access
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[Journal Article] Novel positively charged nanoparticle labeling for in vivo imaging of adipose tissue-derived stem cells.2014
Author(s)
Yukawa H, Nakagawa S, Yoshizumi Y, Watanabe M, Saito H, Miyamoto Y, Noguchi H, Oishi K, Ono K, Sawada M, Kato I, Onoshima D, Obayashi M, Hayashi Y, Kaji N, Ishikawa T, Hayashi S, Baba Y.
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Journal Title
PLoS One.
Volume: 9
Issue: 11
Pages: e110142-e110142
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Ultrafast and wide range analysis of DNA molecules using rigid network structure of solid nanowires2014
Author(s)
S. Rahong, T. Yasui, T. Yanagida, K. Nagashima, M. Kanai, A. Klamchuen, G. Meng, Y. He, F. Zhuge, N. Kaji, T. Kawai, Y. Baba
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Journal Title
Scientific Reports
Volume: 4
Issue: 1
Pages: 5252-5252
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] DNA Manipulation and Separation in Sublithographic-Scale Nanowire Array2013
Author(s)
Takao YASUI, Sakon RAHONG, Koki MOTOYAMA, Takeshi YANAGIDA, Qiong WU, Noritada KAJI, Masaki KANAI, Kentaro DOI, Kazuki NAGASHIMA, Masanobu TOKESHI, Masateru TANIGUCHI, Satoyuki KAWANO, Tomoji KAWAI, and Yoshinobu BABA
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Journal Title
ACS Nano
Volume: 7
Issue: 4
Pages: 3029-3035
DOI
Related Report
Peer Reviewed
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[Presentation] A Millisecond Micro-RNA Extraction Technique for Nanopore-based Nucleic Acids Sequencing2014
Author(s)
Qiong Wu, Takao Yasui, Sakon Rahong, Takeshi Yanagida, Masaki Kanai, Noritada Kaji, Manabu Tokeshi, Kazuki Nagashima, Tomoji Kawai, Yoshinobu Baba
Organizer
The 18th International Conference on Miniaturized System for Chemistry and Life Sciences (uTAS2014)
Place of Presentation
San Antonio, Texas
Year and Date
2014-10-29
Related Report
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