| Budget Amount *help |
¥26,650,000 (Direct Cost: ¥20,500,000、Indirect Cost: ¥6,150,000)
Fiscal Year 2014: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2013: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
Fiscal Year 2012: ¥14,170,000 (Direct Cost: ¥10,900,000、Indirect Cost: ¥3,270,000)
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| Outline of Final Research Achievements |
This research project aims to develop an in-plane nanopore device for single-molecule DNA sequencing that incorporates electrophoresis control of DNA motions through the pore. For this purpose, a new method was developed for evaluating single-molecule dynamics in a nanochannel that is based on simultaneous measurements of the cross-channel ionic current and fluorescence. Moreover, two nanoelectrodes were used to create an electric field inside a fluidic channel. It was demonstrated that this transverse electric field could slow-down the DNA electrophoretic speed by up to 1/500. On the other hand, it was found that the trapping field interferes with the electric field used for the tunneling current measurements. In order to suppress the interference effects, insulator-protected nanoelectrode systems were developed, which were proven to be useful in probing tunneling current with accuracy in a liquid environment.
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