| Budget Amount *help |
¥23,010,000 (Direct Cost: ¥17,700,000、Indirect Cost: ¥5,310,000)
Fiscal Year 2014: ¥10,660,000 (Direct Cost: ¥8,200,000、Indirect Cost: ¥2,460,000)
Fiscal Year 2013: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2012: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
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| Outline of Final Research Achievements |
The identity of all of the cells that comprise induviduals are defined by the cellular epigenom in the nuclei. Due to technical limitation,genome-wide analysis of binding sites of transcriptional regulators are almost limited to cell types for which many cells are abvailable, including hematopoietic cells and cultured cells. I established a method for ChIP-seq of transcriptiopn factors from small number of cells and enabled identification of the genome-wide binding sites of transcription factor at 1/100 ~ 1/1000 time previous scale. Using thise method, Using this method, I determined binding sites of two key transcription factors for germ cell specificatin pathway, BLIMP1 and T (Brachyury), in vitro. In addition, I determined representative histone modifications associated to active transcription and repression, and revealed landscape of chromatin dynamics during the PGC specifciation in vitro.
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