| Budget Amount *help |
¥26,910,000 (Direct Cost: ¥20,700,000、Indirect Cost: ¥6,210,000)
Fiscal Year 2014: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000)
Fiscal Year 2013: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
Fiscal Year 2012: ¥9,620,000 (Direct Cost: ¥7,400,000、Indirect Cost: ¥2,220,000)
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| Outline of Final Research Achievements |
In this research project, we developed a new method for screening non-standard macrocyclic peptide drugs that target cell-surface molecules such as membrane proteins. Normally, target molecules need to be immobilized on magnetic beads for applying in-vitro display method combined with random peptide library. However, immobilization of membrane proteins is generally difficult because isolation and solubilization of such proteins are impossible in many cases. Therefore, in this project, we developed a new in-vitro display method that utilizes cultured cells and/or baculovirus expressing membrane proteins on their surface instead of purified proteins immobilized on beads. By using this method, we could successfully obtain macrocyclic peptides that target CD20, IL28RA, Claudin-1 and 4.
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