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Development of Rapid and Specific Bacterial Sensing Method Using Molecularly Imprinted Polymer

Research Project

Project/Area Number 24685013
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypePartial Multi-year Fund
Research Field Analytical chemistry
Research InstitutionOsaka Prefecture University

Principal Investigator

TOKONAMI Shiho  大阪府立大学, 21世紀科学研究機構, 講師 (60535491)

Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥26,520,000 (Direct Cost: ¥20,400,000、Indirect Cost: ¥6,120,000)
Fiscal Year 2014: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2013: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2012: ¥17,030,000 (Direct Cost: ¥13,100,000、Indirect Cost: ¥3,930,000)
Keywordsバクテリア / 迅速検出 / センサ / 分子鋳型 / 機能性高分子
Outline of Final Research Achievements

In this study, we tried to develop a novel bacterial detection method in which target bacterial surface chemical structures are precisely transferred at a molecular level. The bacterial cavities were successfully created on the polypyrrole film by doping and dedoping bacteria via electrochemical technique. The combination of this film with dielectrophoresis allowed us to detect both gram-positive and gram-negative bacteria specifically within minutes.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • Research Products

    (54 results)

All 2015 2014 2013 2012 Other

All Journal Article (9 results) (of which Peer Reviewed: 9 results,  Acknowledgement Compliant: 1 results) Presentation (37 results) (of which Invited: 3 results) Book (3 results) Remarks (2 results) Patent(Industrial Property Rights) (3 results) (of which Overseas: 1 results)

  • [Journal Article] Development of Bacteria Sensor Using Overoxidized Polypyrrole Molecularly Imprinted Film and Its Application2015

    • Author(s)
      S. Tokonmi, T. Nishino, H. Shiigi, T. Nagaoka
    • Journal Title

      Chemical Sensors

      Volume: 31 Pages: 115-117

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Development of an Observation Platform for Bacterial Activity Using Polypyrrole Films Doped with Bacteria2015

    • Author(s)
      L.Q. Dung, M. Takai, S. Suekuni, S. Tokonami, T. Nishino, H. Shiigi, T. Nagaoka
    • Journal Title

      Analytical Chemistry

      Volume: 87 Issue: 7 Pages: 4047-4052

    • DOI

      10.1021/acs.analchem.5b00544

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Detection of Biomaterials and Bacteria Using Functionalized Nano-and Micro-Spaces2015

    • Author(s)
      S. Tokonami, T. Iida, H. Shiigi, T. Nagaoka
    • Journal Title

      Bunseki Kagaku

      Volume: in press

    • NAID

      130005107516

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Construction of nanoantennas on the bacterial outer membrane2014

    • Author(s)
      H. Shiigi, M. Fukuda, T. Tono, K. Takada, T. Okada, L. Q. Dung, Y. Hatsuoka, T. Kinoshita, M. Takai, S. Tokonami, H. Nakao, T. Nishino, Y. Yamamoto. T. Nagaoka
    • Journal Title

      Chemical Communications

      Volume: 50 Issue: 47 Pages: 6252-6255

    • DOI

      10.1039/c4cc01204f

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Recognition of gram-negative and gram-positive bacteria with a functionalized conducting polymer film2014

    • Author(s)
      S. Tokonami, Y. Nakadoi, H. Nakata, S. Takami, T. Kadoma, H. Shiigi, T. Nagaoka
    • Journal Title

      Research on Chemical Intermediates

      Volume: published online Issue: 6 Pages: 2327-2335

    • DOI

      10.1007/s11164-014-1609-6

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Label-Free and Selective Bacteria Detection Using a Film with Transferred Bacterial Configuration2013

    • Author(s)
      S. Tokonami, Y. Nakadoi, K. Saimatsu, M. Takahashi, M. Ikemizu, T. Kadoma, L. Q. Dung, H. Shiigi, T. Nagaoka
    • Journal Title

      Analytical Chemistry

      Volume: 85 Issue: 10 Pages: 4925-4929

    • DOI

      10.1021/ac3034618

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Placement of Nanospace on an Electrode for Biosensing2012

    • Author(s)
      H. Shiig ,S. Tokonami, Y. Yamamoto, T. Nagaoka
    • Journal Title

      Analytical Sciences

      Volume: 28 Issue: 11 Pages: 1037-1048

    • DOI

      10.2116/analsci.28.1037

    • NAID

      10031126396

    • ISSN
      0910-6340, 1348-2246
    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Entrapment of Whole Cell Bacteria into Conducting Polymers2012

    • Author(s)
      T. Nagaoka, H. Shiig S. Tokonami, K.Saimatsu
    • Journal Title

      Journal of Flow Injection Analysis

      Volume: 29 Pages: 7-10

    • NAID

      40019886495

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Vertical Immobilization of Viable Bacilliform Bacteria into Polyryrrole Films2012

    • Author(s)
      S. Tokonami, K. Saimatsu, Yu Nakadoi, M.Furuta, H. Shiigi, T. Nagaoka
    • Journal Title

      Analytical Sciences

      Volume: 28 Pages: 319-321

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Presentation] 細菌を吸着させた電極のボルタンメトリ2015

    • Author(s)
      L.Q.Dung, 床波志保, 西野智昭, 椎木 弘, 長岡 勉
    • Organizer
      電気化学会第82回大会
    • Place of Presentation
      横浜国立大学
    • Year and Date
      2015-03-17
    • Related Report
      2014 Annual Research Report
  • [Presentation] 過酸化ポリピロール分子鋳型膜を用いる細菌センサの開発と応用2015

    • Author(s)
      床波志保, 西野智昭, 椎木 弘, 長岡 勉
    • Organizer
      電気化学会第82回大会
    • Place of Presentation
      横浜国立大学
    • Year and Date
      2015-03-17
    • Related Report
      2014 Annual Research Report
  • [Presentation] 誘電泳動と細胞鋳型膜による選択的捕捉機構の理論解析2015

    • Author(s)
      田村 守, 中田啓之, 床波志保, 飯田琢也
    • Organizer
      第62回応用物理学会春季学術講演会
    • Place of Presentation
      名古屋国際会議場
    • Year and Date
      2015-03-12
    • Related Report
      2014 Annual Research Report
  • [Presentation] バクテリア・テンプレート細孔を有する導電性ポリマ膜の作製とバクテリア検出への応用2014

    • Author(s)
      長岡 勉  床波志保 、西野智昭 、椎木 弘 、 L.Q.Dung
    • Organizer
      第24回日本MRS年次大会
    • Place of Presentation
      横浜市開港記念会館
    • Year and Date
      2014-12-12
    • Related Report
      2014 Annual Research Report
  • [Presentation] Preparation of Bacteria-Responsive Polymer Membranes and Their Sensing Abilities2014

    • Author(s)
      H. Nakata, T. Iwanami, S. Miyata, T. Fujikawa, H. Shiigi, T. Nagaoka, S.Tokonami
    • Organizer
      19th International Conference on Flow Injection Analysis and Related Techniques
    • Place of Presentation
      ACROS Fukuoka
    • Year and Date
      2014-11-30
    • Related Report
      2014 Annual Research Report
  • [Presentation] Electrochemical Characteristics of Poly(3,4-Ethylenedioxythiophene) Doped with Bacteria2014

    • Author(s)
      L. Q. Dung, T. Nishino, S. Tokonami, H. Shiigi, T. Nagaoka
    • Organizer
      19th International Conference on Flow Injection Analysis and Related Techniques
    • Place of Presentation
      ACROS Fukuoka
    • Year and Date
      2014-11-30
    • Related Report
      2014 Annual Research Report
  • [Presentation] 分子構造転写技術を利用した細菌検出法2014

    • Author(s)
      床波志保
    • Organizer
      大阪府立大学の技術シーズ紹介
    • Place of Presentation
      大阪府立大学
    • Year and Date
      2014-10-17
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Highly-selective bacteria detection using conductive polymer film2014

    • Author(s)
      H. Nakata, S. Tokonami
    • Organizer
      4th TKU-ECUST-OPU-KIST International Symposium
    • Place of Presentation
      TamKang University, Taiwan
    • Year and Date
      2014-09-25
    • Related Report
      2014 Annual Research Report
  • [Presentation] 微生物応答性高分子膜の作製とセンシング2014

    • Author(s)
      中田啓之, 藤岡一志, 山中幹宏, 原 圭太, 椎木 弘, 長岡 勉, 床波志保
    • Organizer
      日本分析化学会 第63年会
    • Place of Presentation
      広島大学
    • Year and Date
      2014-09-17
    • Related Report
      2014 Annual Research Report
  • [Presentation] Formation of Nanoantenna on a Bacterium2014

    • Author(s)
      Y. Hastuoka, M. Fukuda, T. Kinoshita, S. Tokonami, H. Nakao, T. Nishino, H. Shiigi, T. Nagaoka
    • Organizer
      13th European Vacuum Conference
    • Place of Presentation
      Aveiro, Portugal
    • Year and Date
      2014-09-08
    • Related Report
      2014 Annual Research Report
  • [Presentation] Bacteria‐Imprinted Conducting‐Polymers for Appications to Bacteria Monitoring Platforms and Sensors2014

    • Author(s)
      L. Q. Dung, H. Shiigi, T. Nishino, S. Tokonami, T. Nagaoka
    • Organizer
      13th European Vacuum Conference
    • Place of Presentation
      Aveiro, Portugal
    • Year and Date
      2014-09-08
    • Related Report
      2014 Annual Research Report
  • [Presentation] 微生物形状転写技術の開発とセンサ応用2014

    • Author(s)
      中田啓之, 沼田紘志, 高見星司, 門真哲也, 椎木 弘, 長岡 勉, 床波志保
    • Organizer
      第74回分析化学討論会
    • Place of Presentation
      日本大学工学部
    • Year and Date
      2014-05-24
    • Related Report
      2014 Annual Research Report
  • [Presentation] Rapid and Selective Bacteria Detection Using Conductive Polymer Films2014

    • Author(s)
      S.Tokonami, H. Nakata, M. Ikemoto, T. Kadoma, H. Shiigi, T. Nagaoka
    • Organizer
      38th International Symposium on Envronmental Analytical Chemistry
    • Place of Presentation
      Lausanne, Switzerland
    • Year and Date
      2014-05-17
    • Related Report
      2014 Annual Research Report
  • [Presentation] High-Resolution Bacterial Outer Structure Transfer for Bacteria Recognition2013

    • Author(s)
      S. Tokonami
    • Organizer
      The First OPU-TKU International Symposium
    • Place of Presentation
      大阪府立大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] Label-free Bacteria Sensor Using Polymer Film and Its Application to Real Samples2013

    • Author(s)
      H. Nakata, S. Tokonami
    • Organizer
      The First OPU-TKU International Symposium
    • Place of Presentation
      大阪府立大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] Bacteria Detection Using a Film with Transfereed Bacterial Configuration2013

    • Author(s)
      S. Tokonami, S. Takami, H. Shiigi, T. Nagaoka
    • Organizer
      ICFIA2013
    • Place of Presentation
      ポルト ポルトガル
    • Related Report
      2013 Annual Research Report
  • [Presentation] Bacteria-imprinted conducting-polymer based sensors/detectors2013

    • Author(s)
      L. Q. Dung, H. Shiigi, S. Tokonami, T. Nagaoka
    • Organizer
      ICFIA2013
    • Place of Presentation
      ポルト ポルトガル
    • Related Report
      2013 Annual Research Report
  • [Presentation] Immobilization of bacteria into conducting polymer film2013

    • Author(s)
      L. Q. Dung, K. Saimatsu, S. Tokonami, H. Shiigi, T. Nagaoka
    • Organizer
      ICFIA2013
    • Place of Presentation
      ポルト ポルトガル
    • Related Report
      2013 Annual Research Report
  • [Presentation] Label-free Bacteria Sensor Using an Overoxidied Polypyrrole Film2013

    • Author(s)
      H. Nakata, S. Takami, T. Kadoma, H. Shiigi, T. Nagaoka, S. Tokonami
    • Organizer
      JASIS Conference 2013
    • Place of Presentation
      幕張メッセ国際展示場
    • Related Report
      2013 Annual Research Report
  • [Presentation] Immobilization of Bacteria into Poly(3,4-ethylenedioxythiophene) (PEDOT) Film2013

    • Author(s)
      L. Q. Dung, K. Saimatsu, Y. Hatsuoka, S. Tokonami , H. Shiigi, T. Nagaoka
    • Organizer
      ASIANALYSIS XII
    • Place of Presentation
      九州大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] Fabrication of Functionalized Micro-Spaces for Label-Free Bacteria Detection2013

    • Author(s)
      H. Nakata, S. Takami, T. Kadoma, H. Shiigi, T. Nagaoka, S. Tokonami
    • Organizer
      JSAMA2013
    • Place of Presentation
      上海 中国
    • Related Report
      2013 Annual Research Report
  • [Presentation] 過酸化ポリピロールを利用した細菌検出と実分析への展開2013

    • Author(s)
      中田啓之, 床波志保
    • Organizer
      生体分子機能解析のための走査型プローブ顕微鏡手法研究部会 若手の会 2013せんだい研究会
    • Place of Presentation
      仙台
    • Related Report
      2013 Annual Research Report
  • [Presentation] ポリピロールに固定化したバクテリアの呼吸活性の電気化学的評価2013

    • Author(s)
      細末健太, 陶国智史, 床波志保, 椎木 弘, 長岡 勉
    • Organizer
      2013年電気化学秋季大会
    • Place of Presentation
      東京工業大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] 機能性ナノ・マイクロ空間を利用したDNAおよび細菌検出法の開発2013

    • Author(s)
      床波志保
    • Organizer
      日本分析化学会第62年会
    • Place of Presentation
      近畿大学
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 細菌形状を転写した高分子膜による細菌検出と実分析への展開2013

    • Author(s)
      中田啓之, 高見星司, 門真哲也 ,椎木弘, 長岡勉, 床波志保
    • Organizer
      日本分析化学会第62年会
    • Place of Presentation
      近畿大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] 菌体複合ポリピロール膜によるバイオテンプレートの開発2013

    • Author(s)
      細末健太, L. Q. Dung, 陶国智史, 床波志保, 椎木 弘, 長岡 勉
    • Organizer
      日本分析化学会第62年会
    • Place of Presentation
      近畿大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] 一瞬の喜びを求めて2013

    • Author(s)
      床波志保
    • Organizer
      日本分析化学会 近畿支部 第7回夏期セミナー
    • Place of Presentation
      花王株式会社 有田研修所
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 導電性高分子を利用した細菌検出2013

    • Author(s)
      中田啓之, 中土井祐, 高橋茉里, 池水麦平, 門真哲也, 椎木 弘, 長岡 勉, 床波志保
    • Organizer
      第73回分析化学討論会
    • Place of Presentation
      北海道大学函館キャンパス
    • Related Report
      2013 Annual Research Report
  • [Presentation] Creation of Functional Micro-Spaces for Sensor Applications2013

    • Author(s)
      S. Tokonami, Y. Nakadoi, H. Nakata, M. Takahashi, M. Ikemizu, T. Kadoma, H. Shiigi, T. Nagaoka
    • Organizer
      The Second NanoSquare Week
    • Place of Presentation
      大阪府立大学
    • Related Report
      2012 Annual Research Report
  • [Presentation] 細菌検出のためのテーラーメイド型マイクロ空間の創成2012

    • Author(s)
      床波志保,中土井祐,中田啓之,細末健太,推木弘,長岡勉
    • Organizer
      第50回フローインジェクション分析講演会
    • Place of Presentation
      徳島
    • Year and Date
      2012-11-16
    • Related Report
      2012 Annual Research Report
  • [Presentation] Label-Free and Rapid DNA Analysis Using Open Bridge-Structured Gold NanoparticleArray2012

    • Author(s)
      S. Tokonami, H. Shiigi, T. Nagaoka
    • Organizer
      GOLD2012
    • Place of Presentation
      東京
    • Related Report
      2012 Annual Research Report
  • [Presentation] Label-Free Bacteria Detection by Using Overoxidied Polypyrrol Film2012

    • Author(s)
      S. Tokonami, Y. Nakadoi, K. Saimatsu、H. Shiigi, T Naeaoka
    • Organizer
      MIP2012
    • Place of Presentation
      パリ
    • Related Report
      2012 Annual Research Report
  • [Presentation] 微生物形状認識型センサの開発2012

    • Author(s)
      中土井祐,床波志保,高橋茉里,池水麦平,門真哲也,細末健太,椹木弘,長岡勉
    • Organizer
      第33回日本食品微生物学会学術総会
    • Place of Presentation
      福岡
    • Related Report
      2012 Annual Research Report
  • [Presentation] 微生物形状認識型迅速センサの開発2012

    • Author(s)
      中田啓之,床波志保
    • Organizer
      生体分子機能解析のための走査型プローブ顕微鏡手法研究部会 若手の会 in 名古屋
    • Place of Presentation
      名古屋
    • Related Report
      2012 Annual Research Report
  • [Presentation] ポリピロール膜を利用した微生物検出の選択性2012

    • Author(s)
      中土井祐,床波志保,高橋莱里,池水麦平,門真哲也,細末健太,椎木弘,長岡勉
    • Organizer
      日本分析化学会第61年会
    • Place of Presentation
      金沢
    • Related Report
      2012 Annual Research Report
  • [Presentation] 菌体形状認識センサ膜の開発2012

    • Author(s)
      中土井祐,床波志保,高橋茉里,池水麦平,門真哲也,細末健太,椎木弘,長岡勉
    • Organizer
      第72回分析化学討論会
    • Place of Presentation
      鹿児島
    • Related Report
      2012 Annual Research Report
  • [Presentation] Electrochemical Devices using Conducting polymer films doped with bacteria

    • Author(s)
      L. Q. Dung, 細末健太, 床波志保, 椎木 弘, 長岡 勉
    • Organizer
      第51回フローインジェクション分析講演会
    • Place of Presentation
      熊本大学
    • Related Report
      2013 Annual Research Report
  • [Book] 導電性高分子を用いて分子の鋳型センサを作る―分子から細菌までの計測―2014

    • Author(s)
      椎木 弘, 床波志保, 長岡 勉
    • Total Pages
      7
    • Publisher
      エヌ・ティー・エス
    • Related Report
      2014 Annual Research Report
  • [Book] 導電性ポリマーを用いる鋳型センサの作製と桿菌センサへの応用2014

    • Author(s)
      椎木 弘, 床波志保, 陳智棟, 長岡 勉
    • Total Pages
      5
    • Publisher
      技術情報協会
    • Related Report
      2014 Annual Research Report
  • [Book] マイクロ空間を有するチップを用いた細菌の検出2014

    • Author(s)
      床波志保, 中田啓之, 椎木 弘, 長岡 勉
    • Total Pages
      6
    • Publisher
      (株)技術情報協会
    • Related Report
      2013 Annual Research Report
  • [Remarks] 大阪府立大学 文部科学省テニュアトラック普及定着プログラム ナノ科学・材料人材育成拠点

    • URL

      http://www.nanosq.21c.osakafu-u.ac.jp/

    • Related Report
      2013 Annual Research Report
  • [Remarks]

    • URL

      http://www.nanosq.21c.osakafu-u.ac.jp/

    • Related Report
      2012 Annual Research Report
  • [Patent(Industrial Property Rights)] 微生物検出用センサー、その製造方法、およびポリマー層2014

    • Inventor(s)
      池水麦平, 床波志保, 椎木 弘, 長岡 勉
    • Industrial Property Rights Holder
      大阪府立大学、シャープ株式会社
    • Industrial Property Rights Type
      特許
    • Filing Date
      2014-03-10
    • Related Report
      2013 Annual Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] 被検出物質の検出装置および方法2013

    • Inventor(s)
      飯田琢也、床波志保
    • Industrial Property Rights Holder
      大阪府立大学
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2013-114312
    • Filing Date
      2013-05-30
    • Related Report
      2013 Annual Research Report
  • [Patent(Industrial Property Rights)] 微生物検出用センサー、その製造方法、およびポリマー層2013

    • Inventor(s)
      池水麦平、床波志保, 椎木弘、長岡勉
    • Industrial Property Rights Holder
      公立大学法人大阪府立大学シャープ株式会社
    • Industrial Property Number
      2013-069739
    • Filing Date
      2013-03-28
    • Related Report
      2012 Annual Research Report

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Published: 2012-04-24   Modified: 2019-07-29  

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