Development of Rapid and Specific Bacterial Sensing Method Using Molecularly Imprinted Polymer

• Project/Area Number: 24685013
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Partial Multi-year Fund
• Research Field: Analytical chemistry
• Research Institution: Osaka Prefecture University
• Principal Investigator: TOKONAMI Shiho 大阪府立大学, 21世紀科学研究機構, 講師 (60535491)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥26,520,000 (Direct Cost: ¥20,400,000、Indirect Cost: ¥6,120,000); Fiscal Year 2014: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2013: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2012: ¥17,030,000 (Direct Cost: ¥13,100,000、Indirect Cost: ¥3,930,000)
• Keywords: バクテリア / 迅速検出 / センサ / 分子鋳型 / 機能性高分子

Outline of Final Research Achievements

In this study, we tried to develop a novel bacterial detection method in which target bacterial surface chemical structures are precisely transferred at a molecular level. The bacterial cavities were successfully created on the polypyrrole film by doping and dedoping bacteria via electrochemical technique. The combination of this film with dielectrophoresis allowed us to detect both gram-positive and gram-negative bacteria specifically within minutes.

Research Products

• [Journal Article] Development of Bacteria Sensor Using Overoxidized Polypyrrole Molecularly Imprinted Film and Its Application (2015)
  • Author(s): S. Tokonmi, T. Nishino, H. Shiigi, T. Nagaoka
  • Journal Title: Chemical Sensors Volume: 31 Pages: 115-117
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Development of an Observation Platform for Bacterial Activity Using Polypyrrole Films Doped with Bacteria (2015)
  • Author(s): L.Q. Dung, M. Takai, S. Suekuni, S. Tokonami, T. Nishino, H. Shiigi, T. Nagaoka
  • Journal Title: Analytical Chemistry Volume: 87 Issue: 7 Pages: 4047-4052
  • DOI: 10.1021/acs.analchem.5b00544
  • Peer Reviewed
• [Journal Article] Detection of Biomaterials and Bacteria Using Functionalized Nano-and Micro-Spaces (2015)
  • Author(s): S. Tokonami, T. Iida, H. Shiigi, T. Nagaoka
  • Journal Title: Bunseki Kagaku Volume: in press
  • NAID: 130005107516
  • Peer Reviewed
• [Journal Article] Construction of nanoantennas on the bacterial outer membrane (2014)
  • Author(s): H. Shiigi, M. Fukuda, T. Tono, K. Takada, T. Okada, L. Q. Dung, Y. Hatsuoka, T. Kinoshita, M. Takai, S. Tokonami, H. Nakao, T. Nishino, Y. Yamamoto. T. Nagaoka
  • Journal Title: Chemical Communications Volume: 50 Issue: 47 Pages: 6252-6255
  • DOI: 10.1039/c4cc01204f
  • Peer Reviewed
• [Journal Article] Recognition of gram-negative and gram-positive bacteria with a functionalized conducting polymer film (2014)
  • Author(s): S. Tokonami, Y. Nakadoi, H. Nakata, S. Takami, T. Kadoma, H. Shiigi, T. Nagaoka
  • Journal Title: Research on Chemical Intermediates Volume: published online Issue: 6 Pages: 2327-2335
  • DOI: 10.1007/s11164-014-1609-6
  • Peer Reviewed
• [Journal Article] Label-Free and Selective Bacteria Detection Using a Film with Transferred Bacterial Configuration (2013)
  • Author(s): S. Tokonami, Y. Nakadoi, K. Saimatsu, M. Takahashi, M. Ikemizu, T. Kadoma, L. Q. Dung, H. Shiigi, T. Nagaoka
  • Journal Title: Analytical Chemistry Volume: 85 Issue: 10 Pages: 4925-4929
  • DOI: 10.1021/ac3034618
  • Peer Reviewed
• [Journal Article] Placement of Nanospace on an Electrode for Biosensing (2012)
  • Author(s): H. Shiig ,S. Tokonami, Y. Yamamoto, T. Nagaoka
  • Journal Title: Analytical Sciences Volume: 28 Issue: 11 Pages: 1037-1048
  • DOI: 10.2116/analsci.28.1037
  • NAID: 10031126396
  • ISSN: 0910-6340, 1348-2246
  • Peer Reviewed
• [Journal Article] Entrapment of Whole Cell Bacteria into Conducting Polymers (2012)
  • Author(s): T. Nagaoka, H. Shiig S. Tokonami, K.Saimatsu
  • Journal Title: Journal of Flow Injection Analysis Volume: 29 Pages: 7-10
  • NAID: 40019886495
  • Peer Reviewed
• [Journal Article] Vertical Immobilization of Viable Bacilliform Bacteria into Polyryrrole Films (2012)
  • Author(s): S. Tokonami, K. Saimatsu, Yu Nakadoi, M.Furuta, H. Shiigi, T. Nagaoka
  • Journal Title: Analytical Sciences Volume: 28 Pages: 319-321
  • Peer Reviewed
• [Presentation] 細菌を吸着させた電極のボルタンメトリ (2015)
  • Author(s): L.Q.Dung, 床波志保, 西野智昭, 椎木 弘, 長岡 勉
  • Organizer: 電気化学会第82回大会
  • Place of Presentation: 横浜国立大学
  • Year and Date: 2015-03-17
• [Presentation] 過酸化ポリピロール分子鋳型膜を用いる細菌センサの開発と応用 (2015)
  • Author(s): 床波志保, 西野智昭, 椎木 弘, 長岡 勉
  • Organizer: 電気化学会第82回大会
  • Place of Presentation: 横浜国立大学
  • Year and Date: 2015-03-17
• [Presentation] 誘電泳動と細胞鋳型膜による選択的捕捉機構の理論解析 (2015)
  • Author(s): 田村 守, 中田啓之, 床波志保, 飯田琢也
  • Organizer: 第62回応用物理学会春季学術講演会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2015-03-12
• [Presentation] バクテリア・テンプレート細孔を有する導電性ポリマ膜の作製とバクテリア検出への応用 (2014)
  • Author(s): 長岡 勉 床波志保 、西野智昭 、椎木 弘 、 L.Q.Dung
  • Organizer: 第24回日本ＭＲＳ年次大会
  • Place of Presentation: 横浜市開港記念会館
  • Year and Date: 2014-12-12
• [Presentation] Preparation of Bacteria-Responsive Polymer Membranes and Their Sensing Abilities (2014)
  • Author(s): H. Nakata, T. Iwanami, S. Miyata, T. Fujikawa, H. Shiigi, T. Nagaoka, S.Tokonami
  • Organizer: 19th International Conference on Flow Injection Analysis and Related Techniques
  • Place of Presentation: ACROS Fukuoka
  • Year and Date: 2014-11-30
• [Presentation] Electrochemical Characteristics of Poly(3,4-Ethylenedioxythiophene) Doped with Bacteria (2014)
  • Author(s): L. Q. Dung, T. Nishino, S. Tokonami, H. Shiigi, T. Nagaoka
  • Organizer: 19th International Conference on Flow Injection Analysis and Related Techniques
  • Place of Presentation: ACROS Fukuoka
  • Year and Date: 2014-11-30
• [Presentation] 分子構造転写技術を利用した細菌検出法 (2014)
  • Author(s): 床波志保
  • Organizer: 大阪府立大学の技術シーズ紹介
  • Place of Presentation: 大阪府立大学
  • Year and Date: 2014-10-17
  • Invited
• [Presentation] Highly-selective bacteria detection using conductive polymer film (2014)
  • Author(s): H. Nakata, S. Tokonami
  • Organizer: 4th TKU-ECUST-OPU-KIST International Symposium
  • Place of Presentation: TamKang University, Taiwan
  • Year and Date: 2014-09-25
• [Presentation] 微生物応答性高分子膜の作製とセンシング (2014)
  • Author(s): 中田啓之, 藤岡一志, 山中幹宏, 原 圭太, 椎木 弘, 長岡 勉, 床波志保
  • Organizer: 日本分析化学会 第63年会
  • Place of Presentation: 広島大学
  • Year and Date: 2014-09-17
• [Presentation] Formation of Nanoantenna on a Bacterium (2014)
  • Author(s): Y. Hastuoka, M. Fukuda, T. Kinoshita, S. Tokonami, H. Nakao, T. Nishino, H. Shiigi, T. Nagaoka
  • Organizer: 13th European Vacuum Conference
  • Place of Presentation: Aveiro, Portugal
  • Year and Date: 2014-09-08
• [Presentation] Bacteria‐Imprinted Conducting‐Polymers for Appications to Bacteria Monitoring Platforms and Sensors (2014)
  • Author(s): L. Q. Dung, H. Shiigi, T. Nishino, S. Tokonami, T. Nagaoka
  • Organizer: 13th European Vacuum Conference
  • Place of Presentation: Aveiro, Portugal
  • Year and Date: 2014-09-08
• [Presentation] 微生物形状転写技術の開発とセンサ応用 (2014)
  • Author(s): 中田啓之, 沼田紘志, 高見星司, 門真哲也, 椎木 弘, 長岡 勉, 床波志保
  • Organizer: 第74回分析化学討論会
  • Place of Presentation: 日本大学工学部
  • Year and Date: 2014-05-24
• [Presentation] Rapid and Selective Bacteria Detection Using Conductive Polymer Films (2014)
  • Author(s): S.Tokonami, H. Nakata, M. Ikemoto, T. Kadoma, H. Shiigi, T. Nagaoka
  • Organizer: 38th International Symposium on Envronmental Analytical Chemistry
  • Place of Presentation: Lausanne, Switzerland
  • Year and Date: 2014-05-17
• [Presentation] High-Resolution Bacterial Outer Structure Transfer for Bacteria Recognition (2013)
  • Author(s): S. Tokonami
  • Organizer: The First OPU-TKU International Symposium
  • Place of Presentation: 大阪府立大学
• [Presentation] Label-free Bacteria Sensor Using Polymer Film and Its Application to Real Samples (2013)
  • Author(s): H. Nakata, S. Tokonami
  • Organizer: The First OPU-TKU International Symposium
  • Place of Presentation: 大阪府立大学
• [Presentation] Bacteria Detection Using a Film with Transfereed Bacterial Configuration (2013)
  • Author(s): S. Tokonami, S. Takami, H. Shiigi, T. Nagaoka
  • Organizer: ICFIA2013
  • Place of Presentation: ポルト ポルトガル
• [Presentation] Bacteria-imprinted conducting-polymer based sensors/detectors (2013)
  • Author(s): L. Q. Dung, H. Shiigi, S. Tokonami, T. Nagaoka
  • Organizer: ICFIA2013
  • Place of Presentation: ポルト ポルトガル
• [Presentation] Immobilization of bacteria into conducting polymer film (2013)
  • Author(s): L. Q. Dung, K. Saimatsu, S. Tokonami, H. Shiigi, T. Nagaoka
  • Organizer: ICFIA2013
  • Place of Presentation: ポルト ポルトガル
• [Presentation] Label-free Bacteria Sensor Using an Overoxidied Polypyrrole Film (2013)
  • Author(s): H. Nakata, S. Takami, T. Kadoma, H. Shiigi, T. Nagaoka, S. Tokonami
  • Organizer: JASIS Conference 2013
  • Place of Presentation: 幕張メッセ国際展示場
• [Presentation] Immobilization of Bacteria into Poly(3,4-ethylenedioxythiophene) (PEDOT) Film (2013)
  • Author(s): L. Q. Dung, K. Saimatsu, Y. Hatsuoka, S. Tokonami , H. Shiigi, T. Nagaoka
  • Organizer: ASIANALYSIS XII
  • Place of Presentation: 九州大学
• [Presentation] Fabrication of Functionalized Micro-Spaces for Label-Free Bacteria Detection (2013)
  • Author(s): H. Nakata, S. Takami, T. Kadoma, H. Shiigi, T. Nagaoka, S. Tokonami
  • Organizer: JSAMA2013
  • Place of Presentation: 上海 中国
• [Presentation] 過酸化ポリピロールを利用した細菌検出と実分析への展開 (2013)
  • Author(s): 中田啓之, 床波志保
  • Organizer: 生体分子機能解析のための走査型プローブ顕微鏡手法研究部会 若手の会 2013せんだい研究会
  • Place of Presentation: 仙台
• [Presentation] ポリピロールに固定化したバクテリアの呼吸活性の電気化学的評価 (2013)
  • Author(s): 細末健太, 陶国智史, 床波志保, 椎木 弘, 長岡 勉
  • Organizer: 2013年電気化学秋季大会
  • Place of Presentation: 東京工業大学
• [Presentation] 機能性ナノ・マイクロ空間を利用したDNAおよび細菌検出法の開発 (2013)
  • Author(s): 床波志保
  • Organizer: 日本分析化学会第62年会
  • Place of Presentation: 近畿大学
  • Invited
• [Presentation] 細菌形状を転写した高分子膜による細菌検出と実分析への展開 (2013)
  • Author(s): 中田啓之, 高見星司, 門真哲也 ,椎木弘, 長岡勉, 床波志保
  • Organizer: 日本分析化学会第62年会
  • Place of Presentation: 近畿大学
• [Presentation] 菌体複合ポリピロール膜によるバイオテンプレートの開発 (2013)
  • Author(s): 細末健太, L. Q. Dung, 陶国智史, 床波志保, 椎木 弘, 長岡 勉
  • Organizer: 日本分析化学会第62年会
  • Place of Presentation: 近畿大学
• [Presentation] 一瞬の喜びを求めて (2013)
  • Author(s): 床波志保
  • Organizer: 日本分析化学会 近畿支部 第7回夏期セミナー
  • Place of Presentation: 花王株式会社 有田研修所
  • Invited
• [Presentation] 導電性高分子を利用した細菌検出 (2013)
  • Author(s): 中田啓之, 中土井祐, 高橋茉里, 池水麦平, 門真哲也, 椎木 弘, 長岡 勉, 床波志保
  • Organizer: 第73回分析化学討論会
  • Place of Presentation: 北海道大学函館キャンパス
• [Presentation] Creation of Functional Micro-Spaces for Sensor Applications (2013)
  • Author(s): S. Tokonami, Y. Nakadoi, H. Nakata, M. Takahashi, M. Ikemizu, T. Kadoma, H. Shiigi, T. Nagaoka
  • Organizer: The Second NanoSquare Week
  • Place of Presentation: 大阪府立大学
• [Presentation] 細菌検出のためのテーラーメイド型マイクロ空間の創成 (2012)
  • Author(s): 床波志保,中土井祐,中田啓之,細末健太,推木弘,長岡勉
  • Organizer: 第50回フローインジェクション分析講演会
  • Place of Presentation: 徳島
  • Year and Date: 2012-11-16
• [Presentation] Label-Free and Rapid DNA Analysis Using Open Bridge-Structured Gold NanoparticleArray (2012)
  • Author(s): S. Tokonami, H. Shiigi, T. Nagaoka
  • Organizer: GOLD2012
  • Place of Presentation: 東京
• [Presentation] Label-Free Bacteria Detection by Using Overoxidied Polypyrrol Film (2012)
  • Author(s): S. Tokonami, Y. Nakadoi, K. Saimatsu、H. Shiigi, T Naeaoka
  • Organizer: MIP2012
  • Place of Presentation: パリ
• [Presentation] 微生物形状認識型センサの開発 (2012)
  • Author(s): 中土井祐,床波志保,高橋茉里,池水麦平,門真哲也,細末健太,椹木弘,長岡勉
  • Organizer: 第33回日本食品微生物学会学術総会
  • Place of Presentation: 福岡
• [Presentation] 微生物形状認識型迅速センサの開発 (2012)
  • Author(s): 中田啓之,床波志保
  • Organizer: 生体分子機能解析のための走査型プローブ顕微鏡手法研究部会 若手の会 in 名古屋
  • Place of Presentation: 名古屋
• [Presentation] ポリピロール膜を利用した微生物検出の選択性 (2012)
  • Author(s): 中土井祐,床波志保,高橋莱里,池水麦平,門真哲也,細末健太,椎木弘,長岡勉
  • Organizer: 日本分析化学会第61年会
  • Place of Presentation: 金沢
• [Presentation] 菌体形状認識センサ膜の開発 (2012)
  • Author(s): 中土井祐,床波志保,高橋茉里,池水麦平,門真哲也,細末健太,椎木弘,長岡勉
  • Organizer: 第72回分析化学討論会
  • Place of Presentation: 鹿児島
• [Presentation] Electrochemical Devices using Conducting polymer films doped with bacteria
  • Author(s): L. Q. Dung, 細末健太, 床波志保, 椎木 弘, 長岡 勉
  • Organizer: 第51回フローインジェクション分析講演会
  • Place of Presentation: 熊本大学
• [Book] 導電性高分子を用いて分子の鋳型センサを作る―分子から細菌までの計測― (2014)
  • Author(s): 椎木 弘, 床波志保, 長岡 勉
  • Total Pages: 7
  • Publisher: エヌ・ティー・エス
• [Book] 導電性ポリマーを用いる鋳型センサの作製と桿菌センサへの応用 (2014)
  • Author(s): 椎木 弘, 床波志保, 陳智棟, 長岡 勉
  • Total Pages: 5
  • Publisher: 技術情報協会
• [Book] マイクロ空間を有するチップを用いた細菌の検出 (2014)
  • Author(s): 床波志保, 中田啓之, 椎木 弘, 長岡 勉
  • Total Pages: 6
  • Publisher: (株）技術情報協会
• [Remarks] 大阪府立大学 文部科学省テニュアトラック普及定着プログラム ナノ科学・材料人材育成拠点
  • URL: http://www.nanosq.21c.osakafu-u.ac.jp/
• [Remarks]
  • URL: http://www.nanosq.21c.osakafu-u.ac.jp/
• [Patent(Industrial Property Rights)] 微生物検出用センサー、その製造方法、およびポリマー層 (2014)
  • Inventor(s): 池水麦平, 床波志保, 椎木 弘, 長岡 勉
  • Industrial Property Rights Holder: 大阪府立大学、シャープ株式会社
  • Industrial Property Rights Type: 特許
  • Filing Date: 2014-03-10
  • Overseas
• [Patent(Industrial Property Rights)] 被検出物質の検出装置および方法 (2013)
  • Inventor(s): 飯田琢也、床波志保
  • Industrial Property Rights Holder: 大阪府立大学
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2013-114312
  • Filing Date: 2013-05-30
• [Patent(Industrial Property Rights)] 微生物検出用センサー、その製造方法、およびポリマー層 (2013)
  • Inventor(s): 池水麦平、床波志保, 椎木弘、長岡勉
  • Industrial Property Rights Holder: 公立大学法人大阪府立大学シャープ株式会社
  • Industrial Property Number: 2013-069739
  • Filing Date: 2013-03-28