Development of fluorescent photoswitchable molecules toward RESOLFT super-resolution fluorescence microscope
| Project/Area Number |
24685021
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Partial Multi-year Fund |
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| Research Field |
Functional materials chemistry
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| Research Institution | Kumamoto University (2014-2015)
Hokkaido University (2012-2013) |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥22,100,000 (Direct Cost: ¥17,000,000、Indirect Cost: ¥5,100,000)
Fiscal Year 2014: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2013: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2012: ¥13,000,000 (Direct Cost: ¥10,000,000、Indirect Cost: ¥3,000,000)
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| Keywords | 蛍光 / フォトクロミズム / 超解像蛍光イメージング / ジアリールエテン / 蛍光スイッチング / 超解像顕微鏡 / ナノ粒子 / 可視光 / エネルギー移動 / 超解像蛍光顕微鏡 / スイッチング |
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| Outline of Final Research Achievements |
We tried to develop the suitable fluorescent photoswitchable molecules, which have photoreactivity for visible-light and show the stable fluorescence photoswitching even in the aqueous conditions, toward RESOLFT super-resolution fluorescence microscope in this study.
In this study, we successfully prepared a visible-light reactive diarylethene (DAE) derivative by introducing an aromatic dye at the reactive carbon atom of the DAE unit, optimizing orbital level of each component, and controlling the mutual orientation of the aromatic dye and the DAE unit.
In addition, we successfully prepared a highly efficient fluorescent photoswitchable nanoparticle (NP). Such NPs show the non-linear fluorescence photoswitching behavior at very low photoreaction conversion yield. This “giant amplification of fluorescence photoswitching” represent an extremely promising and challenging step toward the application for RESOLFT super-resolution fluorescence microscope.
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Report
(5 results)
Research Products
(39 results)
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[Journal Article] Giant Amplification of Photoswitching by a Few Photons in Fluorescent Photochromic Organic Nanoparticles2016
Author(s)
J. Su, T. Fukaminato, J.-P. Placial, T. Onodera, R. Suzuki, H. Oikawa, A. Brosseau, F. Brisset, R. Pansu, K. Nakatani, R. Métivier
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Journal Title
Angewandte Chemie - International Edition
Volume: 55
Issue: 11
Pages: 3662-3666
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Presentation] Amplified Photoswitching in Fluorescent Photochromic Molecules and Nanomaterials2015
Author(s)
K. Nakatani, R. Métivier, S. Maisonneuve, J. Xie, P. Yu, K. Ouhenia-Ouadahi, J. Su, T. Fukaminato, J.-P. Placial, A. Brosseau, J. Audibert, R. Pansu
Organizer
2015 International Chemical Congress of Pacific Basin Societies
Place of Presentation
Hawaii Convention Center, Honolulu, Hawaii
Year and Date
2015-12-15
Related Report
Int'l Joint Research
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[Presentation] Development of Photochromic Molecules2015
Author(s)
T. Fukaminato, T. Hirose, K. Matsuda, M. Irie
Organizer
2015 International Chemical Congress of Pacific Basin Societies
Place of Presentation
Hawaii Convention Center, Honolulu, Hawaii
Year and Date
2015-12-15
Related Report
Int'l Joint Research
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[Presentation] Amplification of Fluorescence Photoswitching in Organic and Hybrid Nanoparticles2015
Author(s)
R. Métivier, J. Su, K. Ouhenia, S. Maisonneuve, C. Pavageau, J.-P. Placial, A. Brosseau, R. Pansu, J. Xie, K. Nakatani, P. Yu, T. Fukaminato
Organizer
27th International Conference on Photochemistry 2015
Place of Presentation
ICC JEJU, Jeju Island, Korea
Year and Date
2015-06-28
Related Report
Int'l Joint Research
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