| Budget Amount *help |
¥22,100,000 (Direct Cost: ¥17,000,000、Indirect Cost: ¥5,100,000)
Fiscal Year 2014: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2013: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2012: ¥13,000,000 (Direct Cost: ¥10,000,000、Indirect Cost: ¥3,000,000)
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| Outline of Final Research Achievements |
We tried to develop the suitable fluorescent photoswitchable molecules, which have photoreactivity for visible-light and show the stable fluorescence photoswitching even in the aqueous conditions, toward RESOLFT super-resolution fluorescence microscope in this study.
In this study, we successfully prepared a visible-light reactive diarylethene (DAE) derivative by introducing an aromatic dye at the reactive carbon atom of the DAE unit, optimizing orbital level of each component, and controlling the mutual orientation of the aromatic dye and the DAE unit.
In addition, we successfully prepared a highly efficient fluorescent photoswitchable nanoparticle (NP). Such NPs show the non-linear fluorescence photoswitching behavior at very low photoreaction conversion yield. This “giant amplification of fluorescence photoswitching” represent an extremely promising and challenging step toward the application for RESOLFT super-resolution fluorescence microscope.
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