Fluorescent probes to visualize molecular processes in the cell

• Project/Area Number: 24685027
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Partial Multi-year Fund
• Research Field: Chemistry related to living body
• Research Institution: The University of Tokyo
• Principal Investigator: MORITOSHI Sato 東京大学, 総合文化研究科, 准教授 (00323501)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥25,610,000 (Direct Cost: ¥19,700,000、Indirect Cost: ¥5,910,000); Fiscal Year 2014: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000); Fiscal Year 2013: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000); Fiscal Year 2012: ¥10,140,000 (Direct Cost: ¥7,800,000、Indirect Cost: ¥2,340,000)
• Keywords: 蛍光 / 超解像 / プローブ / 細胞 / イメージング / 蛍光タンパク質 / タンパク質間相互作用 / 回折限界

Outline of Final Research Achievements

In this study, we have developed novel fluorescent probes to visualize protein-protein interactions at the super-resolution. The probes get fluorescent only when protein-protein interactions take place. We also conducted protein engineering of a blue light photoreceptor and explored a novel near-infrared fluorescent proteins for long-term, super-resolution imaging of living cells.