| Budget Amount *help |
¥25,610,000 (Direct Cost: ¥19,700,000、Indirect Cost: ¥5,910,000)
Fiscal Year 2014: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2013: ¥10,140,000 (Direct Cost: ¥7,800,000、Indirect Cost: ¥2,340,000)
Fiscal Year 2012: ¥9,230,000 (Direct Cost: ¥7,100,000、Indirect Cost: ¥2,130,000)
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| Outline of Final Research Achievements |
Functional 19F MRI probes were developed in order to visualize biological functions in deep regions of living animals. First, to improve the sensitivity of conventional small-molecule 19F MRI probes, new nanoparticle-based material was developed. This nanoparticle consists of perfluorocarbon core and silica shell. Then, the attenuation method of the 19F MRI signal of this nanomateria was developed by modifying the surface with lots of Gd3+ complexes. By introducing hydrolase substrate with the linker between a Gd3+ complex and the silica surface, highly sensitive nanoparticle-based 19F MRI probes to detect enzyme activities were developed. By using one of the probes, enzyme activities in a living animal was visualized by 19F MRI.
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