• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Development of highly sensitive in vivo imaging technology based on advanced functional nanoparticle design

Research Project

Project/Area Number 24685028
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypePartial Multi-year Fund
Research Field Chemistry related to living body
Research InstitutionOsaka University

Principal Investigator

Mizukami Shin  大阪大学, 工学(系)研究科(研究院), 准教授 (30420433)

Project Period (FY) 2012-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥25,610,000 (Direct Cost: ¥19,700,000、Indirect Cost: ¥5,910,000)
Fiscal Year 2014: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2013: ¥10,140,000 (Direct Cost: ¥7,800,000、Indirect Cost: ¥2,340,000)
Fiscal Year 2012: ¥9,230,000 (Direct Cost: ¥7,100,000、Indirect Cost: ¥2,130,000)
Keywords可視化プローブ / MRI / コア-シェルナノ粒子 / ナノ粒子 / イメージング
Outline of Final Research Achievements

Functional 19F MRI probes were developed in order to visualize biological functions in deep regions of living animals. First, to improve the sensitivity of conventional small-molecule 19F MRI probes, new nanoparticle-based material was developed. This nanoparticle consists of perfluorocarbon core and silica shell. Then, the attenuation method of the 19F MRI signal of this nanomateria was developed by modifying the surface with lots of Gd3+ complexes. By introducing hydrolase substrate with the linker between a Gd3+ complex and the silica surface, highly sensitive nanoparticle-based 19F MRI probes to detect enzyme activities were developed. By using one of the probes, enzyme activities in a living animal was visualized by 19F MRI.

Report

(5 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • Research Products

    (38 results)

All 2016 2015 2014 2013 2012 Other

All Journal Article (19 results) (of which Peer Reviewed: 19 results,  Open Access: 4 results,  Acknowledgement Compliant: 2 results) Presentation (18 results) (of which Int'l Joint Research: 2 results,  Invited: 5 results) Remarks (1 results)

  • [Journal Article] Visualization and Functional Regulation of Live Cell Proteins Based on Labeling Probe Design2016

    • Author(s)
      水上進, 菊地和也
    • Journal Title

      YAKUGAKU ZASSHI

      Volume: 136 Issue: 1 Pages: 21-27

    • DOI

      10.1248/yakushi.15-00225-4

    • NAID

      130005115189

    • ISSN
      0031-6903, 1347-5231
    • Year and Date
      2016-01-01
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Selective Labeling of Proteins on Living Cell Membranes Using Fluorescent Nanodiamond Probes.2016

    • Author(s)
      Shingo Sotoma, Jun Iimura, Ryuji Igarashi, Koichiro M. Hirosawa, Hidenori Ohnishi, Shin Mizukami, Kazuya Kikuchi, Takahiro K. Fujiwara, Masahiro Shirakawa and Hidehito Tochio
    • Journal Title

      Nanomaterials

      Volume: 6 Issue: 4 Pages: 56-56

    • DOI

      10.3390/nano6040056

    • NAID

      120005848250

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] An Enzyme-Responsive Metal-Enhanced Near-Infrared Fluorescence Sensor Based on Functionalized Gold Nanoparticles2015

    • Author(s)
      Zhanghua Zeng, Shin Mizukami, Katsumasa Fujita, Kazuya Kikuchi
    • Journal Title

      Chem. Sci.

      Volume: 6 Issue: 8 Pages: 4934-4939

    • DOI

      10.1039/c5sc01850a

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Actavataqble 19F MRI nanoparticle probes for the detection of reducing environments2015

    • Author(s)
      Nkamura, T., Matsushita, H., Sugihara, F., Yoshioka, Y., Mizukami, S. and Kikuchi, K.
    • Journal Title

      Angewandte Chemi International Edition

      Volume: 54 Issue: 3 Pages: 1007-1010

    • DOI

      10.1002/anie.201409365

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Mesoporous slilica nanoparticles for 19F magnetic resonance imaging, fluorescence imaging, and drug deliverry2015

    • Author(s)
      Nakamura, T., Sugihara, F., Matsushita, H., Yoshioka, Y., <izukami, S., and Kikuchi, K.
    • Journal Title

      Chemical Science

      Volume: 6 Issue: 3 Pages: 1986-1990

    • DOI

      10.1039/c4sc03549f

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Nonlinear Fluorescence Imaging by Using Phtoinduced Charge Separation2015

    • Author(s)
      Kentaro Mochizuki, Lanting Shi, Shin Mizukami, Masahito Yamanaka, Mamoru Tanabe, Wei-Tao Gong, Almar F Palonpon, Shogo Kawano, Satoshi Kawata, Kazuya Kikuchi, Katsumasa Fujita
    • Journal Title

      Jpn. J. Appl. Phys.

      Volume: 54 Issue: 4 Pages: 042403-042403

    • DOI

      10.7567/jjap.54.042403

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Ratiometric MRI Sensors Based on Core-Shell Nanoparticles for Quantitative pH Imaging2014

    • Author(s)
      Satoshi Okada, Shin Mizukami, Takao Sakata, Yutaka Matsumura, Yoshichika Yoshioka, Kazuya Kikuchi
    • Journal Title

      Adv. Mater.

      Volume: 26 Issue: 19 Pages: 2989-2992

    • DOI

      10.1002/adma.201305018

    • NAID

      40021302212

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Membrane Protein CNNM4-Dependent Mg2+ Efflux Suppresses Tumor Progression2014

    • Author(s)
      Yosuke Funato, Daisuke Yamazaki, Shin Mizukami, Lisa Du, Kazuya Kikuchi, Hiroaki Miki
    • Journal Title

      J. Clin. Invest.

      Volume: 124 Issue: 12 Pages: 5398-5410

    • DOI

      10.1172/jci76614

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of Functional <sup>1</sup>H MRI Probes Based on Nanoparticle Design2013

    • Author(s)
      水上進
    • Journal Title

      YAKUGAKU ZASSHI

      Volume: 133 Issue: 3 Pages: 351-354

    • DOI

      10.1248/yakushi.12-00239-1

    • NAID

      130003361923

    • ISSN
      0031-6903, 1347-5231
    • Year and Date
      2013-03-01
    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Nanospherical Polymer as an MRI Sensor without Paramagnetic or Superparamagnetic Species2013

    • Author(s)
      Satoshi Okada, Shin Mizukami, Yutaka Matsumura, Yoshichika Yoshioka, Kazuya Kikuchi
    • Journal Title

      Dalton Trans.

      Volume: 42 Issue: 45 Pages: 15864-15867

    • DOI

      10.1039/c3dt50378j

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Efficient Development of Luminescent Lanthanide(III) Complexes by Solid-Phase Synthesis and On-Resin Screening2013

    • Author(s)
      Tatsuya Nakamura, Shin Mizukami, Miho Tanaka, Kazuya Kikuchi
    • Journal Title

      Chem. Asian J.

      Volume: 8 Issue: 11 Pages: 2685-2690

    • DOI

      10.1002/asia.201300759

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of Luminescent Coelenterazine Derivatives Activatable by β-Galactosidase for Monitoring Dual Gene Expression2013

    • Author(s)
      Lindberg E, Mizukami S, Ibata K, Miyawaki A, Kikuchi K
    • Journal Title

      Chem. Eur. J.

      Volume: 19 Issue: 44 Pages: 14970-14976

    • DOI

      10.1002/chem.201302002

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of Cell-Impermeable Coelenterazine Derivatives2013

    • Author(s)
      Lindberg E, Mizukami S, Ibata K, Fukano T, Miyawaki A, Kikuchi K
    • Journal Title

      Chem. Sci.

      Volume: 4 Issue: 12 Pages: 4395-4400

    • DOI

      10.1039/c3sc51985f

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Multifunctional Core-shell Silica Nanoparticles for Highly Sensitive ^<19>F MRI2013

    • Author(s)
      H. Matsushita, S. Mizukami, F. Sugihara, Y. Nakanishi, Y. Yoshioka & K. Kikuchi
    • Journal Title

      Angew. Chem. Int. Ed

      Volume: 52(in press) Issue: 4 Pages: 52-52

    • DOI

      10.1002/anie.201308500

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Small-Molecule-Based Protein-Labeling Technology in Live Cell Studies : Probe-Design Concepts and Applications2013

    • Author(s)
      S. Mizumkami, Y. Hori & K. Kikuchi
    • Journal Title

      Acc. Chem. Res

      Volume: 46(in press) Issue: 1 Pages: 247-256

    • DOI

      10.1021/ar400135f

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] PH Induced Dual "OFF-ON-OFF″Switch : Influence of Suitably Placed Carboxylic Acid2013

    • Author(s)
      Kalyan K. Sadhu, Shin Mizukami. Kazuya Kikuchi
    • Journal Title

      Org. Biomol. Chem

      Volume: 11 Pages: 563-568

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 細胞蛋白質への機能性分子ラベリング技術2013

    • Author(s)
      水上進・菊地和也
    • Journal Title

      未来材料

      Volume: 2 Pages: 5-10

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Simple and Real-time Colorimetric Assay for Glycosidases Activity Using Functionalized Gold Nanoparticles and Its Application for Inhibitor Screening2012

    • Author(s)
      Z. Zeng, S. Mizukami & K. Kikuchi
    • Journal Title

      Anal. Chem.

      Volume: 84 Issue: 21 Pages: 9089-9085

    • DOI

      10.1021/ac301677v

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] ^<19>F MRI monitoring of Gene Expression in Living Cells via Cell Surface β-Lactamase Activity2012

    • Author(s)
      松下尚嗣、水上進、杉原文徳、白川昌宏、菊地和也
    • Journal Title

      ChemBioChem

      Volume: 13 Issue: 11 Pages: 1579-1583

    • DOI

      10.1002/cbic.201200331

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Presentation] Development of protein-tag-based research tools in live cell imaging2015

    • Author(s)
      Shin Mizukami, Kazuya Kikuchi
    • Organizer
      Pacifichem 2015
    • Place of Presentation
      ホノルル(米国)
    • Year and Date
      2015-12-15
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Development of activatable 19F MRI contrast agents based on paramagnetic relaxation enhancement2015

    • Author(s)
      Kazuki Akazawa, Fuminori Sugihara, Yoshichika Yoshioka, Shin Mizukami, Kazuya Kikuchi
    • Organizer
      Pacifichem 2015
    • Place of Presentation
      ホノルル(米国)
    • Year and Date
      2015-12-15
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 機能性金ナノ粒子設計に基づく酵素反応検出近赤外蛍光センサーの開発2015

    • Author(s)
      水上進、Zhanghua Zeng、藤田克昌、菊地和也
    • Organizer
      第9回バイオ関連化学シンポジウム
    • Place of Presentation
      熊本大学黒髪南地区キャンパス(熊本県・熊本市)
    • Year and Date
      2015-09-10
    • Related Report
      2015 Annual Research Report
  • [Presentation] 刺激応答性19F MRIナノプローブの開発2015

    • Author(s)
      石田健一郎、赤澤一樹、杉原文徳、吉岡芳親、水上進、菊地和也
    • Organizer
      日本ケミカルバイオロジー学会 第10回年会
    • Place of Presentation
      東北大学川内キャンパス(宮城県・仙台市)
    • Year and Date
      2015-06-10
    • Related Report
      2015 Annual Research Report
  • [Presentation] 常磁性緩和促進効果に基づいた刺激応答性19F MRI造影剤の開発2015

    • Author(s)
      赤澤一樹、中村竜也、杉原文徳、吉岡芳親、水上進、菊地和也
    • Organizer
      第10回 日本分子イメージング学会 学術集会
    • Place of Presentation
      タワーホール船堀(東京都)
    • Year and Date
      2015-05-20
    • Related Report
      2015 Annual Research Report
  • [Presentation] ラベル化分子設計に基づく蛋白質の可視化と機能制御2015

    • Author(s)
      水上進,菊地和也
    • Organizer
      日本薬学会 シンポジウム
    • Place of Presentation
      兵庫、神戸学院大学
    • Year and Date
      2015-03-26
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Development of smart 19F MRI probes for in vivo imaging2014

    • Author(s)
      Shin Mizukami
    • Organizer
      China/Japan Young Chemist Forum
    • Place of Presentation
      中国、北京大学
    • Year and Date
      2014-08-05
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 蛋白質ラベル化技術を用いた生細胞蛋白質の光活性化2014

    • Author(s)
      水上進,吉村彰真,菊地和也
    • Organizer
      日本ケミカルバイオロジー学会 第9回年会
    • Place of Presentation
      大阪、大阪大学豊中キャンパス
    • Year and Date
      2014-06-12
    • Related Report
      2014 Annual Research Report
  • [Presentation] pH定量を可能とするコア-シェルナノ粒子型レシオMRIセンサー2014

    • Author(s)
      水上進,岡田智,坂田孝夫,松村豊,吉岡芳親,菊地和也
    • Organizer
      第9回日本分子イメージング学会学術集会
    • Place of Presentation
      大阪、千里ライフサイエンスセンター
    • Year and Date
      2014-05-22 – 2014-05-23
    • Related Report
      2014 Annual Research Report
  • [Presentation] Protein labeling technology for investigating small number molecules in living cells2013

    • Author(s)
      水上進
    • Organizer
      日本生物物理学会
    • Place of Presentation
      京都、京都国際会館
    • Year and Date
      2013-10-29
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 機能性プローブ設計に基づく分子イメージング技術の開発2013

    • Author(s)
      水上進
    • Organizer
      東京工業大学大学院生命理工学研究科 生体分子サイエンスセミナー
    • Place of Presentation
      神奈川、東京工業大学すずかけ台キャンパス
    • Year and Date
      2013-08-29
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 蛋白質ラベル化技術に基づく生体分子の少数活性化へのアプローチ2013

    • Author(s)
      水上進
    • Organizer
      日本顕微鏡学会第69回学術講演会
    • Place of Presentation
      大阪、ホテル阪急エキスポパーク
    • Year and Date
      2013-05-22
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] ^<19>F MRIプローブの開発~酵素活性検出からin vivo応用まで2013

    • Author(s)
      水上進
    • Organizer
      日本薬学会第133年会シンポジウム
    • Place of Presentation
      横浜(招待講演)
    • Year and Date
      2013-03-30
    • Related Report
      2012 Annual Research Report
  • [Presentation] Development of ^<19>F MRI Probes Based on Coordination Chemistry and Nanomaterials2013

    • Author(s)
      Shin Mizukami
    • Organizer
      日本化学会-Asian International Symposium
    • Place of Presentation
      草津(招待講演)
    • Year and Date
      2013-03-24
    • Related Report
      2012 Annual Research Report
  • [Presentation] 蛋白質機能を可視化する分子の設計と開発2012

    • Author(s)
      水上進
    • Organizer
      新学術領域研究若手合同シンポジウム「配位プログラム」×「融合マテリアル」
    • Place of Presentation
      東京(招待講演)
    • Year and Date
      2012-12-21
    • Related Report
      2012 Annual Research Report
  • [Presentation] Development of Protein Labeling Technology Based on Mutant β-Lactamase2012

    • Author(s)
      Shin Mizukami. Yuri Akimoto, Akimasa Yoshimura, Mitsuru Ueda, Kazuya Kikuchi
    • Organizer
      新学術領域「少数性生物学」第1回国際会議
    • Place of Presentation
      台北(台湾)
    • Year and Date
      2012-10-16
    • Related Report
      2012 Annual Research Report
  • [Presentation] ナノバイオプローブ設計を基盤とする分子イメージング技術開発2012

    • Author(s)
      水上進
    • Organizer
      日本化学会生体機能関連化学部会若手の会サマースクール
    • Place of Presentation
      福岡(招待講演)
    • Year and Date
      2012-07-27
    • Related Report
      2012 Annual Research Report
  • [Presentation] In vivo ^<19>F MRIを可能とするコア-シェルナノ粒子の開発2012

    • Author(s)
      水上進, 中西陽介, 杉原文徳, 向井大陽, 菊地和也
    • Organizer
      日本分子イメージング学会第7回学会総会・学術集会
    • Place of Presentation
      浜松
    • Year and Date
      2012-05-25
    • Related Report
      2012 Annual Research Report
  • [Remarks]

    • URL

      http://www-molpro.mls.eng.osaka-u.acjp/

    • Related Report
      2012 Annual Research Report

URL: 

Published: 2012-04-24   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi