| Budget Amount *help |
¥26,650,000 (Direct Cost: ¥20,500,000、Indirect Cost: ¥6,150,000)
Fiscal Year 2014: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2013: ¥9,620,000 (Direct Cost: ¥7,400,000、Indirect Cost: ¥2,220,000)
Fiscal Year 2012: ¥12,350,000 (Direct Cost: ¥9,500,000、Indirect Cost: ¥2,850,000)
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| Outline of Final Research Achievements |
The aim of this project is to develop a fiber-shaped building unit, named “cell fiber,” for the construction of centimeter-scale 3D tissue constructs. I fabricated cell fibers that have a core of cell-suspended extracellular matrix (ECM) hydrogel and a shell of Ca-alginate hydrogel by using a double co-axial laminar flow microfluidic device. I found out that the choice of core ECM materials is critical to form cell fibers corresponding to various types of cells. Using cell fibers, I demonstrated a fiber-based centimeter-scale 3D tissue constructs woven by our developed micro weaving machine, and bundle-like neural 3D tissue structures. Those fabricated fiber-based 3D constructs maintained their viability and can be applied to in vivo implantation, which suggest that this fiber-based approach would be a powerful method for constructing large-scale spatially-ordered functional tissues.
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