| Budget Amount *help |
¥26,520,000 (Direct Cost: ¥20,400,000、Indirect Cost: ¥6,120,000)
Fiscal Year 2014: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2013: ¥10,140,000 (Direct Cost: ¥7,800,000、Indirect Cost: ¥2,340,000)
Fiscal Year 2012: ¥11,310,000 (Direct Cost: ¥8,700,000、Indirect Cost: ¥2,610,000)
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| Outline of Final Research Achievements |
We developed selective and rapid injection of fluorescence sensor encapsulated in the artificial biomembrane into the cell for intracellular measurement. The biomembrane contains photochromic chemical, 1, 3, 3-Trimethylindolino-6'- nitrobenzopyrylospiran (SP), for optical control of zeta potential. The structure of SP changes between spiropyran (SP) type and merocyanine (MC) type by UV/VIS illumination. Zeta potential of the biomembrne is switched from negative to positive by photoisomerization of SP. The sensor in the positive-charged biomembrane adheres to the cell membrane by electrostatic force since the cell membrane is negative-charged. The cyclic mechanical stimulus using optical tweezers is applied to the cell membrane for rapid injection of the sensor by membrane fusion and endocytosis. We demonstrated selective adhesion of the liposome to the cell membrane by optical control of zeta potential. We also demonstrated short-time injection of 1 um fluorescence sensor.
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