Development of Nanoinjection System and Application for Nematoda

• Project/Area Number: 24686033
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Partial Multi-year Fund
• Research Field: Intelligent mechanics/Mechanical systems
• Research Institution: Nagoya University
• Principal Investigator: Nakajima Masahiro 名古屋大学, 工学(系)研究科(研究院), 助教 (80377837)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥26,650,000 (Direct Cost: ¥20,500,000、Indirect Cost: ¥6,150,000); Fiscal Year 2014: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000); Fiscal Year 2013: ¥8,450,000 (Direct Cost: ¥6,500,000、Indirect Cost: ¥1,950,000); Fiscal Year 2012: ¥11,310,000 (Direct Cost: ¥8,700,000、Indirect Cost: ¥2,610,000)
• Keywords: ナノインジェクション / 線形動物 / 局所操作 / 環境制御型電子顕微鏡 / ナノマニピュレーション / モデル生物 / 細胞解析 / ハイブリッド顕微鏡

Outline of Final Research Achievements

The local injection system was constructed for a model organism Nematoda. The local injection was demonstrated by fixing C. elegans inside a micro-channel. The nanoinjection was demonstrated for C. elegans by the nanomanipulation system inside an environmental scanning electron microscope.

Research Products

• [Presentation] In situ Nanomanipulation with 3D SEM-CT Observation inside Environmental SEM (2015)
  • Author(s): M. Nakajima, M. Takeuchi, T. Fukuda, Y. Hasegawa, Q. Huang
  • Organizer: 2015 Int. Symp. on Micromechatronics and Human Science (MHS 2015)
  • Place of Presentation: Nagoya, Japan
  • Year and Date: 2015-11-23
  • Int'l Joint Research
• [Presentation] Micro-Nanomanipulation System under Environmental Scanning Electron Microscope for C. elegans (2015)
  • Author(s): M. Nakajima
  • Organizer: 5th Int. Conf. on Manipulation, Manu­facturing and Measurement on the Nanoscale (3M-NANO 2015)
  • Place of Presentation: Changchun, China
  • Year and Date: 2015-10-05
  • Int'l Joint Research / Invited
• [Presentation] Survival Microinjection into C. Elegans with in Vivo Observation Based on Micromanipulation (2015)
  • Author(s): M. Nakajima, Y. Ayamura, M. Takeuchi, M. Masaru, N. Hisamoto, S. Pastuhov, Y. Hasegawa, T. Fukuda, Q. Huang
  • Organizer: 2015 IEEE/RSJ Int. Conf. on Intelligent Robotics and Systems (IROS 2015)
  • Place of Presentation: Humburg, Germany
  • Year and Date: 2015-09-28
  • Int'l Joint Research
• [Presentation] 含水状態での環境制御型電顕内SEM-CT撮影 (2015)
  • Author(s): 中島正博，永尾圭，竹内大，久本直毅，長谷川泰久，福田敏男
  • Organizer: 第33回日本ロボット学会 学術講演会
  • Place of Presentation: 東京
  • Year and Date: 2015-09-03
• [Presentation] 線虫への局所マイクロインジェクションにおける生存率の評価 (2015)
  • Author(s): 綾村友貴，中島正博，竹内大，久本直毅，パストゥホフストラヒル，長谷川泰久，福田敏男
  • Organizer: ロボィクス・メカトロニクス講演会2015
  • Place of Presentation: 京都
  • Year and Date: 2015-05-17
• [Presentation] Local Microinjection near Nerve Axon of C. elegans with Hydro-gel Beads Based on Micromanipulation (2014)
  • Author(s): M. Nakajima, Y. Ayamura, M. Takeuchi, N. Hisamoto, P. Strahil, Y. Hasegawa, T. Fukuda
  • Organizer: 2014 IEEE/SICE International Symposium on System Integration
  • Place of Presentation: 東京
  • Year and Date: 2014-12-13 – 2014-12-15
• [Presentation] SEM-CT Observation of Bone from Mice using Environmental-SEM (2014)
  • Author(s): M. Nakajima, M. Takeuchi, Taisuke Masuda, Takshisa Anada, Osamu Suzuki, T. Fukuda, Q. Huang, Y. Hasegawa
  • Organizer: 2014 Int. Symp. on Micromechatronics and Human Science (MHS 2014)
  • Place of Presentation: 名古屋
  • Year and Date: 2014-11-09 – 2014-11-12
• [Presentation] SEM-CTによる線虫の3次元観察とナノマニピュレーション応用 (2014)
  • Author(s): 中島正博，竹内大，久本直毅，福田敏男，長谷川泰久
  • Organizer: 第32回日本ロボット学会学術講演会（RSJ2014）
  • Place of Presentation: 九州
  • Year and Date: 2014-09-04 – 2014-09-06
• [Presentation] 線虫への局所マイクロインジェクションと体内でのゲルビーズ観察 (2014)
  • Author(s): 綾村友貴，中島正博，竹内大，久本直毅，長谷川泰久，福田敏男
  • Organizer: 第32回日本ロボット学会学術講演会（RSJ2014）
  • Place of Presentation: 九州
  • Year and Date: 2014-09-04 – 2014-09-06
• [Presentation] Cross-sectional Imaging of C. elegans by SEM-CT using Environmental SEM for Nanomanipulation (2014)
  • Author(s): M. Nakajima, M. Takeuchi, N. Hisamoto, T. Fukuda, Y. Hasegawa, Q. Huang
  • Organizer: 14th IEEE Int. Conf. on Nanotech. (IEEE-Nano 2014)
  • Place of Presentation: トロント
  • Year and Date: 2014-08-18 – 2014-08-21
• [Presentation] 複数マイクロゲルビーズの線虫への局所マイクロインジェクション (2014)
  • Author(s): 中島正博，中西直哉，竹内大，久本直毅，福田敏男
  • Organizer: ロボティクス・メカトロニクス講演会2014 (Robomech2014)
  • Place of Presentation: 富山
  • Year and Date: 2014-05-25 – 2014-05-29
• [Presentation] マイクロゲルビーズを用いた線虫への局所薬剤インジェクション (2013)
  • Author(s): 中西直哉, 中島正博, 田島寛隆, 久本直毅, 本間道夫, 福田敏男
  • Organizer: ロボティクス・メカトロニクス講演会2013
  • Place of Presentation: 筑波
• [Presentation] Long Survival Observation of C. elegans inside Environmental SEM for Nanomanipulations (2013)
  • Author(s): M. Nakajima, N. Nakanishi, N. Hisamoto, M. Takeuchi, H. Tajima, M. Homma, Q. Huang, T. Fukuda
  • Organizer: 2013 IEEE International Conference on Nanotechnology (IEEE-Nano 2013)
  • Place of Presentation: 北京
• [Presentation] Nano-biomanipulation for C. elagans under Environmental-SEM (2013)
  • Author(s): M. Nakajima
  • Organizer: 2013 IEEE International Conference on Nanotechnology (IEEE-Nano 2013)
  • Place of Presentation: 北京
  • Invited
• [Presentation] 蒸発を抑制することによる生きた線虫の環境制御型電顕観察 (2013)
  • Author(s): 中西直哉, 中島正博, 竹内大, 久本直毅, 本間道夫, 福田敏男
  • Organizer: 第31回 日本ロボット学会 学術講演会
  • Place of Presentation: 東京
• [Presentation] Semi-wet Manipulation of C. elagans in Micro-Nano scale (2013)
  • Author(s): T. Fukuda, M. Nakajima
  • Organizer: 2013 International Conference on Advanced Computer Science and Information Systems (ICACSIS 2013)
  • Place of Presentation: インドネシア
  • Invited
• [Presentation] 環境制御型電子顕微鏡内ナノマニピュレーションによる線虫操作 (2013)
  • Author(s): 中島正博
  • Organizer: 平成25年度 日本表面科学会中部支部研究会
  • Place of Presentation: 金沢
  • Invited
• [Presentation] In-situ Nanomanipulation System forNano Injection into C. elegans (2013)
  • Author(s): M. Nakajima, T. Fukuda
  • Organizer: International Conference on Mechanical, Industrial and Materials Engineering (ICMIME2013)
  • Place of Presentation: バングラデシュ
  • Invited
• [Presentation] Multi-slicing of C. elegans Tissue using Micro-N anocutting Prove based on Nano manipulation (2012)
  • Author(s): Masahiro Nakajima, Hirotaka Hida, Yajing Shen, Mas am Kojima, Kazuo Sato, Toshio Fukuda
  • Organizer: Proceedings of the 2012 12th IEEE Conference on Nanotechnology (IEEE-Nano 2012)
  • Place of Presentation: Birmingham, UK
• [Presentation] Local Nano-injection of Fluorencent Nanc-beads inside C. elegans based on Nanomanipulation (2012)
  • Author(s): Masahiro Nakajima, Takahiro Hirano, Mas aru Kojima, Naoki Hisamoto, Naoya Nakanishi, Hirotaka Tajima, Michiko Homma, Toshio Fukuda
  • Organizer: Proceedings of the 2012 IEEE/RSJ International Conference on Intelligent Robotics and Systems (IROS 2012)
  • Place of Presentation: Vilamoura, Portugal
• [Presentation] Local Injection Probe of Functional Micro-Nano Gel Tools into Caenorha bditis elegans (2012)
  • Author(s): Masahiro Nakajima, Naoya Nakanishi, Naoki, Hisamoto, Hirotaka Tajima, Michio Homma, Toshio Fukuda
  • Organizer: Proceedings of the 20 12 International Symposium on Micromechatronics and Human Science (MHS2012)
  • Place of Presentation: Nagoya, Japan
• [Book] Micro-Nanorobotic Manipulation Systems and Their Applications (2013)
  • Author(s): Toshio Fukuda, Fumihito Arai, Masahiro Nakajima
  • Publisher: Springer