Desgin of Biointerfaces with Protein Refolding Property, Based on Amyloid Fibril Formation
| Project/Area Number |
24686086
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Partial Multi-year Fund |
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| Research Field |
Properties in chemical engineering process/Transfer operation/Unit operation
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| Research Institution | Okayama University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥27,170,000 (Direct Cost: ¥20,900,000、Indirect Cost: ¥6,270,000)
Fiscal Year 2014: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥19,240,000 (Direct Cost: ¥14,800,000、Indirect Cost: ¥4,440,000)
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| Keywords | バイオインターフェース / アミロイド / 生体膜 / タンパク質 / メンブレンチップ / 生体膜晶析 |
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| Outline of Final Research Achievements |
In this study, we developed the noveland medical bio-interfaces with protein refolding property, based on the amyloid fibril formation. We used the variety of phospholipids, detergents, and polymers to investigate their kinetic interfacial properties such as fluidity and interfacial energy. Furthermore, the kinetic parameters including the nucleation time and elongation rate constant for the fibril formation were evaluated and its mechanistic detailes was discussed, taking into consideration of influence of the prepared interfaces. Consequently, it was suggested that the constituent of interfaces should require the molecular fluidity within the interface enough to allow the orientation of proteins into the interior of interfaces.
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Report
(4 results)
Research Products
(45 results)
