Stimuli-responsive nano-shells for caging living cells
| Project/Area Number |
24686094
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Partial Multi-year Fund |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥27,300,000 (Direct Cost: ¥21,000,000、Indirect Cost: ¥6,300,000)
Fiscal Year 2013: ¥8,450,000 (Direct Cost: ¥6,500,000、Indirect Cost: ¥1,950,000)
Fiscal Year 2012: ¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000)
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| Keywords | 細胞工学 / 光応答性材料 / 細胞パターニング / 細胞接着 / 自己集合 / 光分解性材料 / ケージド化学 / ヒドロゲル / 細胞 / 刺激応答性材料 / ケージング / 光分解性保護基 / 交互積層法 / 再生医療 |
|---|
| Research Abstract |
We developed methods for preparing "caged cells", which are covered with the nano-shell consisting of stimuli-responsive polymer complexes to control the cellular functions with external stimuli. In this study, light stimulation was selected as the external stimulus because of its high spatio-temporal resolution, and photo-cleavable nano-shells were challenged to be developed. For the cell-coating material, a photo-degradable hydrogel was produced by self-assembly of biocompatible polymers through a photo-cleavable linker group. Then, the method for coating cellular surfaces with the present photo-degradable self-assembly materials was optimized for tightly caging living cells, and the functions of caged cells such as elongation, adhesion and phagocytosis were successfully confirmed to be controlled by light irradiation.
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Report
(3 results)
Research Products
(57 results)
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[Presentation] 光溶解性タンパク質凝集体の開発2013
Author(s)
石渡晟,山口哲志,高森智史,南畑孝介,長棟輝行
Organizer
第7回バイオ関連化学シンポジウム
Place of Presentation
名古屋,名古屋大学東山キャンパス
Year and Date
2013-09-28
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[Presentation] 光溶解性タンパク質凝集体の開発
Author(s)
石渡 晟, 山口 哲志, 高森 智史, 南畑 孝介, 長棟 輝行
Organizer
第7回バイオ関連化学シンポジウム
Place of Presentation
名古屋大学東山キャンパス, 名古屋
Related Report
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