Molecular mechanism for opposite synaptic transmission in neural circuit of C. elegans

• Project/Area Number: 24687002
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Partial Multi-year Fund
• Research Field: Genetics/Genome dynamics
• Research Institution: Konan University
• Principal Investigator: KUHARA Atsushi 甲南大学, 理工学部, 准教授 (00402412)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥19,370,000 (Direct Cost: ¥14,900,000、Indirect Cost: ¥4,470,000); Fiscal Year 2014: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2013: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000); Fiscal Year 2012: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000)
• Keywords: 線虫 / C. elegans / 温度応答 / 神経回路 / カルシウムイメージング / 神経伝達 / 温度情報処理 / シナプス

Outline of Final Research Achievements

To identify molecular and physiological mechanisms for complicated-synaptic transmission system and compartment of synapse localization, we used simple neural circuit underlying temperature sensation and response. We utilized molecular genetics and optical imaging of various mutants of nematode C. elegans. We found that molecular mechanism for self-activation of glutamate signaling, compartmentalization between pre- and post synapses in single neural process, and novel paradigm for analysis of temperature response.

Research Products

• [Journal Article] Light and pheromone-sensing neuron regulates cold habituation through insulin signaling in C. elegans (2014)
  • Author(s): Ohta, A., Ujisawa T., Sonoda S., Kuhara, A.
  • Journal Title: Nature commun. Volume: 5:4412 Issue: 1 Pages: 1-12
  • DOI: 10.1038/ncomms5412
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Cold tolerance assay for studying cultivation-temperature-dependent cold habituation in C. elegans (2014)
  • Author(s): Ujisawa T., Ohta, A., Okahata M., Sonoda S., Kuhara, A.
  • Journal Title: Protocol Exchange Volume: 0 Pages: 1-7
  • DOI: 10.1038/protex.2014.032
  • Open Access / Acknowledgement Compliant
• [Journal Article] Molecular mechanism for trimetric G protein-coupled thermosensation and synaptic regulation in the temperature response circuit of Caenorhabditis elegans (2013)
  • Author(s): Ohta, A. & Kuhara, A.
  • Journal Title: Neuroscience Research Volume: 76(3) Issue: 3 Pages: 119-124
  • DOI: 10.1016/j.neures.2013.03.008
  • Peer Reviewed
• [Journal Article] Synaptic Polarity Depends on Phosphatidylinositol Signaling Regulated by Myo-inositol Monophosphatase in Caenorhabditis elegans (2012)
  • Author(s): Kimata, T., Tanizawa, Y., Can, Y., Ikeda, S., Kuhara, A., Mori, I.
  • Journal Title: Genetics Volume: (印刷中)(未定) Issue: 2 Pages: 509-521
  • DOI: 10.1534/genetics.111.137844
  • Peer Reviewed
• [Journal Article] A novel and conserved protein AHO-3 is required for thermotactic plasticity associated with feeding states in Caenorhabditis elegans. (2012)
  • Author(s): Nishio, N., Mohri-Shiomi, A., Nishida, Y., Hiramatsu, N., Kodama-Namba, E., Kimura, K.D., Kuhara, A., and Mori, I.
  • Journal Title: Genes Cells Volume: 17 Issue: 5 Pages: 365-386
  • DOI: 10.1111/j.1365-2443.2012.01594.x
  • Peer Reviewed
• [Journal Article] 線虫Caenorhabditis elegansの温度応答を制御する神経情報処理メカニズム (2012)
  • Author(s): 大西憲幸、久原篤
  • Journal Title: 比較生理生化学 Volume: 29(3) Pages: 112-120
  • NAID: 10031123310
  • Peer Reviewed
• [Journal Article] 線虫の温度応答の分子生理メカニズム (2012)
  • Author(s): 久原篤
  • Journal Title: 生物物理 Volume: 52(4) Pages: 200-202
  • NAID: 10030494713
  • Peer Reviewed
• [Presentation] 線虫C. エレガンスを用いた温度感知と記憶の神経機能解析 (2014)
  • Author(s): 久原篤
  • Organizer: 実験動物技術者協会シンポジウム
  • Place of Presentation: 広島
  • Year and Date: 2014-11-29 – 2014-11-30
  • Invited
• [Presentation] Light and pheromone sensoryneuron regulates temperature habituation in C. elegans (2014)
  • Author(s): Kuhara A.
  • Organizer: 12th International Symposium on Molecular and Neural Mechanisms of Taste and Olfactory Perception
  • Place of Presentation: Hakata
  • Year and Date: 2014-11-02 – 2014-11-03
  • Invited
• [Presentation] Neural system for temperature response in C. elegans (2013)
  • Author(s): 久原 篤, 太田 茜, 宇治澤知代, 園田 悟
  • Organizer: 大阪大学蛋白研究所セミナー 光の、光による、光のためのタンパク質科学
  • Place of Presentation: 大阪大学
  • Invited
• [Presentation] Single sensory neuron controls cultivation temperature-dependent cold tolerance in C. elegans (2013)
  • Author(s): Tomoyo Ujisawa, Naoto Kuwahara, Akane Ohta, Atsushi Kuhara
  • Organizer: Neuro2013
  • Place of Presentation: 京都国際会議場
• [Presentation] Identification of genes underlying cultivation temperature-dependent cold tolerance in C. elegans (2013)
  • Author(s): Satoru Sonoda, Hitomi Mizutani, Yukari Kinoshita, Hiromi Nagaya, Yukie Okubo, Tomoyo Ujisawa, Akane Ohta and Atsushi Kuhara
  • Organizer: Neuro2013
  • Place of Presentation: 京都国際会議場
• [Presentation] Cultivation temperature-dependent cold tolerance is regulated by a neuronal hormone insulin that is received by intestine and neuron in C. elegans (2013)
  • Author(s): Akane Ohta, Tomoyo Ujisawa, Takuro Inoue, Naoto Kuwahara, Naho Inoue, Atsushi Kuhara
  • Organizer: Neuro2013
  • Place of Presentation: 京都国際会議場
• [Presentation] Isolation of genes for temperature experience-dependent cold tolerance (2013)
  • Author(s): Satoru Sonoda, Yukari Kinoshita, Shoko Furukawa, Mikiko Endo, Yushuke Uehara, Akane Ohta, Atsushi Kuhara
  • Organizer: 17th International C. elegans Meeting
  • Place of Presentation: UCLA, Los. Angels, USA
• [Presentation] Temperature experience inducing cold tolerance is regulated by insulin signaling in intestine and neuron (2013)
  • Author(s): Akane Ohta, Satoru Sonoda, Tomoyo Ujisawa, Yuko Kobayashi, Hayato Nakamoto and Atsushi Kuhara
  • Organizer: 17th International C. elegans Meeting
  • Place of Presentation: UCLA, Los. Angels, USA
• [Presentation] Light-sensing neuron regulates temperature experience-dependentg cold tolerance (2013)
  • Author(s): Tomoyo Ujisawa,Satoru Sonoda, Tomohiro Ishiwari, Atsushi Kuhara
  • Organizer: 17th International C. elegans Meeting
  • Place of Presentation: UCLA, Los. Angels, USA
• [Presentation] Natural variation of genes modifying temperature responses of C. elegans (2013)
  • Author(s): Shoko Furukawa, Hitomi Mizutani, Akane Ohta, Atsushi Kuhara
  • Organizer: JSCPB2013
  • Place of Presentation: Himeji
• [Presentation] Temperature experience-inducing cold tolerance is regulated by photo-sensoryneuron controlling insulin secretion (2013)
  • Author(s): Akane Ohta, Tomoyo Ujisawa, Satoru Sonoda, Yuko Kobayashi, Tomohiro Ishiwari, Hayato Nakamoto and Atsushi Kuhara
  • Organizer: JSCPB2013
  • Place of Presentation: Himeji
• [Presentation] 線虫C. elegansにおける温度適応を制御する神経システム (2013)
  • Author(s): 太田茜、宇治澤 知代、園田 悟、木下 ゆかり、古川 翔子、遠藤 美喜子、小林 祐子、石割 友博、上原 湧将、久原 篤
  • Organizer: 遺伝学会
  • Place of Presentation: 慶応大学
• [Presentation] Artificial evolution and screening for novel mutants of temperature tolerance in C. elegans (2013)
  • Author(s): 木下 ゆかり、遠藤美喜子、園田悟、太田 茜、久原 篤
  • Organizer: 動物学会
  • Place of Presentation: 岡山大学
• [Presentation] 線虫C. elegansの多機能性ニューロンによる温度適応の制御 (2013)
  • Author(s): 太田茜、宇治澤 知代、園田 悟、小林 祐子、石割 友博、上原 湧将、久原 篤
  • Organizer: 動物学会
  • Place of Presentation: 岡山大学
• [Presentation] Controlling and measuring temperature responses by optogenetics (2013)
  • Author(s): Atsushi Kuhara, Akane Ohta, Tomoyo Ujisawa, Satoru Sonoda
  • Organizer: International Optogenetics symposium
  • Place of Presentation: 慶応大学
  • Invited
• [Presentation] 温度応答・適応の分子神経ネットワーク (2013)
  • Author(s): 太田茜, 宇治澤知代, 園田悟, 久原篤
  • Organizer: 分子生物学会
  • Place of Presentation: 神戸ポートアイランド
  • Invited
• [Presentation] 動物の温度応答の分子遺伝学 (2012)
  • Author(s): 久原篤
  • Organizer: 遺伝学会年会
  • Place of Presentation: 九州大学(招待講演)
  • Year and Date: 2012-09-25
• [Presentation] Seeking molecular and neural mechanisms of temperature response and resistance in C. elegans (2012)
  • Author(s): 宇治澤知代, 園田悟, 大久保幸恵, 水谷仁美, 長屋ひろみ, 桑原直人, 太田茜, 久原篤
  • Organizer: 第50回日本生物物理学会年会
  • Place of Presentation: 名古屋
  • Year and Date: 2012-09-24
• [Presentation] 温度情報処理の神経回路システムの分子生理的解析 (2012)
  • Author(s): 久原篤
  • Organizer: 包括型脳科学研究推進支援ネットワーク 夏のワークショップ
  • Place of Presentation: 仙台
  • Year and Date: 2012-07-26
• [Presentation] Molecular physiological mechanism underlying neural circuit for temperature sensing and memory in C. elegans (2012)
  • Author(s): 久原篤
  • Organizer: 第83回日本動物学会年会
  • Place of Presentation: 大阪
• [Presentation] Temperature-resistance is regulated through sensory signaling in C.clegans (2012)
  • Author(s): 宇治澤知代、太田茜、桑原直人、久原篤
  • Organizer: 第83回日本動物学会年会
  • Place of Presentation: 大阪
• [Presentation] 線虫の低温応答適応に関わる感覚神経系と分子機構 (2012)
  • Author(s): 宇治澤知代、桑原直人、太田茜、久原篤
  • Organizer: 分子生物学会年会
  • Place of Presentation: 福岡
• [Presentation] 動物の感覚と記憶の暗号をひも解く
  • Author(s): 久原篤
  • Organizer: 統合ニューロバイオロジー研究所 シンポジウム
  • Place of Presentation: 神戸、甲南大学
  • Invited
• [Book] Someone (2012)
  • Author(s): 久原篤
  • Total Pages: 31
  • Publisher: リバネス
• [Remarks] 久原研究室ホームページ
  • URL: http://kuharan.com/index.html
• [Remarks] 甲南大学研究者紹介
  • URL: http://researchers.adm.konan-u.ac.jp/html/100000141_ja.html
• [Remarks] 久原研究室ホームページ
  • URL: http://kuharan.com/index.html

• [Remarks] 甲南大学研究者紹介
  • URL: http://researchers.adm.konan-u.ac.jp/html/100000141_ja.html
• [Remarks] 久原研究室ホームページ
  • URL: http://kuharan.com/index.html
• [Remarks] 甲南大学研究者紹介
  • URL: http://researchers.adm.konan-u.ac.jp/html/100000141_ja.html