Construction of multi-scale mapping system of plant tissues and cells prepared by high-pressure freezing and TEM techniques

• Project/Area Number: 24687007
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Partial Multi-year Fund
• Research Field: Morphology/Structure
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: Toyooka Kiminori 国立研究開発法人理化学研究所, 環境資源科学研究センター, 上級研究員 (10360596)
• Research Collaborator: SATO MAYUKO ; MOCHIDA KEIICHI ; NAGATA NORIKO ; MATSUOKA KEN ; HASEZAWA SEIICHIRO ; KUTUSNA NATUMARO ; HIGAKI TAKUMI ; WAKAZAKI MAYUMI ; HASHIMOTO KEI
• Project Period (FY): 2012-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥27,040,000 (Direct Cost: ¥20,800,000、Indirect Cost: ¥6,240,000); Fiscal Year 2015: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000); Fiscal Year 2014: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000); Fiscal Year 2013: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000); Fiscal Year 2012: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
• Keywords: 電子顕微鏡 / オルガネラ / マッピング / 電子地図 / 高圧凍結技法 / タバコ培養細胞 / 細胞内構造 / 電顕マップ / 広域微細構造 / TEM / 自動撮影 / 透過電子顕微鏡 / シロイヌナズナ

Outline of Final Research Achievements

Transmission electron microscopy (TEM) is important for high-resolution ultrastructure observation for organelles, cells and tissues. However, it is unsuitable for comprehensive analyses. I constructed “Electron microscope atlas” to achieve an exhaustive ultrastructural analysis of organelles in model plants by using high-pressure freezing (HPF) and TEM techniques. To take wide and high-resolution TEM images, plant organs and culture cells were frozen by HFP machine and then fix. After embedded in resin, large ultra thin sections were put on a hole grid. Using our developed auto-TEM image acquisition system, I obtained several giga-pixel images of plant organs and culture cells. Moreover, I clarified the ultrastructural and distribution changes of organelles in culture cells, and developed a web-based 2D map “Electron microscope atlas” that can be zoomed and searched.

Research Products

• [Journal Article] Accumulation of fluorescent proteins derived from a trans-Golgi cisternal membrane marker and paramural bodies in interdigitated apoplastic space in Arabidopsis leaf epidermis (2016)
  • Author(s): Akita, K., Kobayashi, M., Sato, M., Kutsuna, N., Ueda, T., Toyooka, K., Nagata, N., Hasezawa, S., and Higaki, T.
  • Journal Title: Protoplasma Volume: in press Issue: 1 Pages: 367-377
  • DOI: 10.1007/s00709-016-0955-1
  • Peer Reviewed / Open Access
• [Journal Article] Haustorial hairs are specialized root hairs that support parasitism in the facultative parasitic plant, Phtheirospermum japonicum (2016)
  • Author(s): Songkui Cui, Takanori Wakatake, Kei Hashimoto, Simon Saucet, Kiminori Toyooka, Satoko Yoshida and Ken Shirasu
  • Journal Title: Plant Physiology Volume: 170 Issue: 3 Pages: 1492-1503
  • DOI: 10.1104/pp.15.01786
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Deficiency of Starch Synthase IIIa and IVb Alters Starch Granule Morphology from Polyhedral to Spherical in Rice Endosperm (2016)
  • Author(s): Toyosawa Y, Kawagoe Y, Matsushima R, Crofts N, Ogawa M, Fukuda M, Kumamaru T, Okazaki Y, Kusano M, Saito K, Toyooka K, Sato M, Ai Y, Jane JL, Nakamura Y, Fujita N
  • Journal Title: Plant Physiol. Volume: 170 Issue: 3 Pages: 1255-1270
  • DOI: 10.1104/pp.15.01232
  • Peer Reviewed
• [Journal Article] Morphological and quantitative changes in mitochondria, plastids, and peroxisomes during the log-to-stationary transition of the growth phase in cultured tobacco BY-2 cells (2016)
  • Author(s): K. Toyooka, M. Sato, M. Wakazaki, K. Matsuoka
  • Journal Title: Plant Signaling & Behavior Volume: 11 Issue: 3 Pages: e1149669-e1149669
  • DOI: 10.1080/15592324.2016.1149669
  • Peer Reviewed / Open Access
• [Journal Article] 光-電子相関顕微鏡法： 蛍光タンパク質標識した細胞小器官を走査電子顕微鏡で捉える (2016)
  • Author(s): 豊岡 公徳
  • Journal Title: 日本植物形態学会誌 Plant Morphology Volume: -
  • NAID: 130006904152
• [Journal Article] SNAC-As, stress-responsive NAC transcription factors, mediate ABA-inducible leaf senescence. (2015)
  • Author(s): Takasaki H, Maruyama K, Takahashi F, Fujita M, Yoshida T, Nakashima K, Myouga F, Toyooka K, Yamaguchi-Shinozaki K, Shinozaki K.
  • Journal Title: Plant Journal Volume: 84 Issue: 6 Pages: 1114-1123
  • DOI: 10.1111/tpj.13067
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Th17 Cell induction by adhesion of microbes to intestinal epithelial cells (2015)
  • Author(s): Atarashi, K., Tanoue, T., Ando. M., Kamada, N., Nagano, Y., Narushima, S., Suda, W., Imaoka, A., Setoyama, H., Nagamori, T., Ishikawa, E., Shima, T., Hara, T., Kado, S., Jinnohara, T., Ohno, H., Kondo, T. et al.
  • Journal Title: Cell Volume: 163 Issue: 2 Pages: 367-380
  • DOI: 10.1016/j.cell.2015.08.058
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Determination of growth stages and metabolic profiles in Brachypodium distachyon for comparison of developmental context with Triticeae crops. (2015)
  • Author(s): Onda, Y., Hashimoto, K., Yoshida, T., Sakurai, T., Sawada, Y., Hirai, M.Y., Toyooka, K., Mochida, K. and Shinozaki, K.
  • Journal Title: Proceedings of the Royal Society B Volume: 282 Issue: 1811 Pages: 1-8
  • DOI: 10.1098/rspb.2015.0964
  • Peer Reviewed / Open Access
• [Journal Article] Semi-automatic organelle detection on transmission electron microscopic images (2015)
  • Author(s): T. Higaki, N. Kutsuna, K. Akita, M. Sato, F. Sawaki, M. Kobayashi, N. Nagata, K. Toyooka, S. Hasezawa
  • Journal Title: SCIENTIFIC REPORTS Volume: 5 Issue: 1 Pages: 7794-7794
  • DOI: 10.1038/srep07794
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] RECG Maintains Plastid and Mitochondrial Genome Stability by Suppressing Extensive Recombination between Short Dispersed Repeats. (2015)
  • Author(s): Odahara, M., Masuda, Y., Sato,M., Wakazaki, M., Harada,C., Toyooka,K., and Sekine,Y.
  • Journal Title: PLOS Genetics Volume: 11 Issue: 3 Pages: e1005080-e1005080
  • DOI: 10.1371/journal.pgen.1005080
  • Peer Reviewed / Open Access
• [Journal Article] 高圧凍結技法を用いた細胞内小胞輸送経路の解明 (2015)
  • Author(s): 豊岡 公徳
  • Journal Title: 医生電顕技術誌 Volume: 28 Pages: 62-63
• [Journal Article] Specific role of phosphatidylglycerol and functional overlaps with other thylakoid lipids in Arabidopsis chloroplast biogenesis. (2014)
  • Author(s): Kobayashi K, Fuji S., Sato M, Toyooka K, Wada H
  • Journal Title: Plant Cell Reports Volume: 34 Issue: 4 Pages: 631-42
  • DOI: 10.1007/s00299-014-1719-z
  • Peer Reviewed / Open Access
• [Journal Article] Five Cyanophora (Cyanophorales, Glaucophyta) species delineated based on morphological and molecular data. (2014)
  • Author(s): Takahashi, T., Sato, M., Toyooka, K., Matsuzaki, R., Kawafune, K., Kawamura, M., Okuda, K. and Nozaki, H.
  • Journal Title: J. Phycol. Volume: 50 Issue: 6 Pages: 1058-1069
  • DOI: 10.1111/jpy.12236
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Wide-range high-resolution transmission electron microscopy reveals morphological and distributional changes of endomembrane compartments during log to stationary transition of growth phase in tobacco BY-2 cells. (2014)
  • Author(s): Toyooka K, Sato M, Kutsuna N, Higaki T, Sawaki F, Wakazaki M, Goto Y, Hasezawa S, Nagata N, Matsuoka K
  • Journal Title: Plant Cell Physiol. Volume: 55 Issue: 9 Pages: 1544-1555
  • DOI: 10.1093/pcp/pcu084
  • Peer Reviewed / Open Access
• [Journal Article] Surface Ornamentation of <i>Cyanophora paradoxa</i> (Cyanophorales, Glaucophyta) Cells as Revealed by Ultra-High Resolution Field Emission Scanning Electron Microscopy (2014)
  • Author(s): Takahashi, T., Sato, M., Toyooka, K. and Nozaki, H.
  • Journal Title: CYTOLOGIA Volume: 79 Issue: 1 Pages: 119-123
  • DOI: 10.1508/cytologia.79.119
  • NAID: 130004448264
  • ISSN: 0011-4545, 1348-7019
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Contribution of NAC transcription factors to plant adaptation to land. (2014)
  • Author(s): Xu, B., Ohtani, M., Yamaguchi, M., Toyooka, K., Wakazaki, M., Sato, M., Kubo, M., Nakano, Y., Sano, R., Hiwatashi, Y., Murata, T., Kurata, T., Yoneda, A., Kato, K., Hasebe, M., and Demura, T.
  • Journal Title: Science Volume: 343 Issue: 6178 Pages: 1505-1508
  • DOI: 10.1126/science.1248417
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Organ-specific quality control of plant peroxisomes is mediated by autophagy. (2014)
  • Author(s): Yoshimoto, K., Shibata, M., Kondo, M., Oikawa, K., Sato, M., Toyooka, K., Shirasu, K., Nishimura, M., and Ohsumi, Y
  • Journal Title: Journal of Cell Science Volume: 127 Pages: 1161-1168
  • DOI: 10.1242/jcs.139709
  • Peer Reviewed / Open Access
• [Journal Article] Development of high resolution TEM image acquisition system by using high-pressure freezing method (2014)
  • Author(s): 豊岡公徳，佐藤繭子，朽名夏麿，永田典子
  • Journal Title: PLANT MORPHOLOGY Volume: 26 Issue: 1 Pages: 3-8
  • DOI: 10.5685/plmorphol.26.3
  • NAID: 130005066550
  • ISSN: 0918-9726, 1884-4154
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] 高圧凍結技法を用いた細胞内小胞輸送経路の解明 (2014)
  • Author(s): 豊岡公徳
  • Journal Title: 医生電顕技術誌 Volume: 28 Pages: 62-63
  • Acknowledgement Compliant
• [Journal Article] 高圧凍結技法を取り入れた広域透過電顕像自動取得システムの開発とその応用 (2014)
  • Author(s): 豊岡公徳，佐藤繭子，朽名夏麿，永田典子
  • Journal Title: Plant Morphology Volume: 印刷中
  • NAID: 130005066550
• [Journal Article] Polarized localization and borate-dependent degradation of the Arabidopsis borate transporter BOR1 in tobacco BY-2 cell (2013)
  • Author(s): Tadashi Gosho, Satoru Asatuma, Kiminori Toyooka, Toru Fujiwara, Ken Matsuoka
  • Journal Title: F1000Reserch Volume: ー Pages: 185-185
  • DOI: 10.12688/f1000research.2-185.v1
  • Peer Reviewed
• [Journal Article] Pleiotropic effect of sigE over-expression on cell morphology, photosynthesis and hydrogen production in Synechocystis sp. PCC 6803 (2013)
  • Author(s): Osanai
  • Journal Title: Plant J Volume: 76 Issue: 3 Pages: 456-465
  • DOI: 10.1111/tpj.12310
  • Peer Reviewed
• [Journal Article] Reconstructing plant cells in 3D by serial section electron tomography. (2013)
  • Author(s): Toyooka, K. Kang, B-H
  • Journal Title: Methods Mol., Biol. Volume: 1080 Pages: 159-170
  • DOI: 10.1007/978-1-62703-643-6_13
  • ISBN: 9781627036429, 9781627036436
• [Journal Article] Reconstructing plant cells in 3D by serial section electron tomography (2013)
  • Author(s): K. Toyoka, B-H, Kang
  • Journal Title: Methods in Mol. Biol. Volume: (In press)
• [Journal Article] Ultrastructure of the rickettsial endosymbiont "MIDORIKO"in the green alga Carteria cerasiformis as revealed by high-pressure freezing and freeze-substitution fixation. (2012)
  • Author(s): Kawafune, K. , Sato, M., Toyooka, K. Nozaki, H
  • Journal Title: Protoplasma Volume: (印刷中) Issue: 4 Pages: 949-953
  • DOI: 10.1007/s00709-012-0469-4
  • Peer Reviewed
• [Journal Article] Micro-CT observations of the 3D distribution of calcium oxalate crystals in cotyledons during maturation and germination in Lotus miyakojimae seeds. (2012)
  • Author(s): Yamauchi, D., Tamaoki, D., Hayami, M., Takeuchi, M., Karahara, I., Sato, M., Toyooka, K., Nishioka, H., Terada, Y., Uesugi, K., Takano, H., Kagoshima, Y., Mineyuki, Y.
  • Journal Title: Journal of Electron Microscopy Volume: (印刷中) Issue: 3 Pages: 353-361
  • DOI: 10.1093/jmicro/dfs079
  • Peer Reviewed
• [Journal Article] The Ets transcription factor Spi-B is essential for the differentiation of intestinal microfold cells (2012)
  • Author(s): Kanaya, T., Hase, K., Takahashi, D., Fukuda, S., Hoshino, K., Sasaki, I., Hemmi, H., Knoop, K. A., Kumar, N., Sato, M., Katsuno, T., Yokosuka, O., Toyooka, K., Nakai, K., Sakamoto, A., Kitahara, Y., Jinnohara, T., McSorley, S. J., Kaisho, T., Williams, I. R., and Ohno, H.
  • Journal Title: Nat Immunol Volume: 13 Issue: 8 Pages: 729-736
  • DOI: 10.1038/ni.2352
  • Peer Reviewed
• [Journal Article] Lysine decarboxylase catalyzes the first step of quinolizidine alkaloid biosynthesis and coevolved with alkaloid production in Leguminosae (2012)
  • Author(s): Somnuk Bunsupa, Kae Katayama, Emi Ikeura, Akira Oikawa, Kiminori Toyooka, Kazuki Saito, Mami Yamazaki.
  • Journal Title: Plant Cell Volume: 24 Issue: 3 Pages: 1202-1216
  • DOI: 10.1105/tpc.112.095885
  • Peer Reviewed
• [Journal Article] Regulation of root greening by light and auxin/cytokinin signaling in Arab idopsis. (2012)
  • Author(s): Kobayashi, K., Baba, S., Obayashi, T. Sato, M., Toyooka, K., Keranen, M., Aro, E. -M, Fukaki, H., Ohta, E., Sugimoto K., Masuda T.
  • Journal Title: Plant Cell Volume: 24 Issue: 3 Pages: 1081-1095
  • DOI: 10.1105/tpc.111.092254
  • Peer Reviewed
• [Presentation] 小胞体から液胞への KDELタンパク 質の大量輸送に ER ボディが関与する ~ギガピクセル TEM 像を用いて~ (2016)
  • Author(s): 橋本恵,成川苗子,若崎眞由美,佐藤 繭子,永田典子,岡本龍史,豊岡公徳
  • Organizer: 第 57 回 日本植物生理学会年会
  • Place of Presentation: 岩手大学
  • Year and Date: 2016-03-18
• [Presentation] 光顕と電顕で植物の成長を担うタンパク質の輸送・分解機構を明らかにする (2015)
  • Author(s): 豊岡公徳
  • Organizer: 日本女子大バイオイメージングセンターシンポジウム2015
  • Place of Presentation: 日本女子大目白キャンパス
  • Year and Date: 2015-12-05
  • Invited
• [Presentation] Ultrastructural analysis of the ER bodies in the Arabidopsis root tip cells: Using Gigapixel images (2015)
  • Author(s): Kiminori Toyooka, Kei Hashimoto, Naeko Narikawa, Mayumi Wakazaki, Mayuko Sato, Noriko Nagata, Takashi Okamoto
  • Organizer: International microscopy workshop on plant sciences 2015
  • Place of Presentation: Himeji
  • Year and Date: 2015-11-24
  • Int'l Joint Research / Invited
• [Presentation] The ER body in the lateral root cap is involved in mass transport of (K/H)DEL proteins to the vacuole: Using Gigapixel TEM images (2015)
  • Author(s): Kiminori Toyooka, Kei Hashimoto, Naeko Narikawa, Mayumi Wakazaki, Mayuko Sato, Noriko Nagata, Takashi Okamoto
  • Organizer: The 2nd EastAsia Microscopy Conference (EAMC2)
  • Place of Presentation: Himeji
  • Year and Date: 2015-11-24
  • Int'l Joint Research
• [Presentation] 光-電子相関顕微鏡法：蛍光タンパク質標識した細胞小器官を走査電子顕微鏡で捉える (2015)
  • Author(s): 豊岡公徳
  • Organizer: 日本植物学会 第 79回大会
  • Place of Presentation: 新潟朱鷺メッセ
  • Year and Date: 2015-09-05
• [Presentation] 電顕アトラスの構築と活用 (2015)
  • Author(s): 豊岡公徳，佐藤繭子，若崎眞由美, 橋本恵, 小林恵, 澤木史江, 朽名夏麿，永田典子
  • Organizer: 日本植物形態学会第 27 回総会・大会
  • Place of Presentation: 新潟
  • Year and Date: 2015-09-05
• [Presentation] Construction of an Arabidopsis electron microscopy atlas (2015)
  • Author(s): TOYOOKA Kiminori, SATO Mayuko, WAKAZAKI Mayumi, HASHIMOTO Kei, KOBAYASHI Megumi, SAWAKI Fumie, KUTSUNA Natsumaro, NAGATA Noriko
  • Organizer: The 26th InternatIonal Conference on Arabidopsis Research
  • Place of Presentation: Paris
  • Year and Date: 2015-07-05
  • Int'l Joint Research
• [Presentation] 理研CSRSにおける電子顕微鏡施設の管理と運営 (2015)
  • Author(s): 豊岡公徳
  • Organizer: 日本顕微鏡学会第71回学術講演会
  • Place of Presentation: 京都国際会館
  • Year and Date: 2015-05-12
• [Presentation] 対数増殖期および定常状態期におけるタバコ培養細胞内オルガネラの超微形態変化 (2015)
  • Author(s): 豊岡公徳、佐藤繭子、若崎眞由美、朽名夏麿、永田典子、松岡健
  • Organizer: 日本生理学会第56回年会
  • Place of Presentation: 東京農業大学世田谷キャンパス
  • Year and Date: 2015-03-16 – 2015-03-18
• [Presentation] シロイヌナズナ根端組織におけるERボディ様構造体の超微形態解析 (2014)
  • Author(s): 橋本恵、成川苗子、佐藤繭子、若崎眞由美、岡本龍史、豊岡公徳
  • Organizer: 日本植物学会第78回大会
  • Place of Presentation: 明治大学生田キャンパス
  • Year and Date: 2014-09-12 – 2014-09-14
• [Presentation] 広域TEM像取得システムを用いたタバコ培養細胞のオルガネラ定量解析 (2014)
  • Author(s): 豊岡公徳、佐藤繭子、若崎眞由美、朽名夏麿、永田典子、松岡健
  • Organizer: 日本植物形態学会第26回総会・大会
  • Place of Presentation: 明治大学生田キャンパス
  • Year and Date: 2014-09-11
• [Presentation] 高圧凍結技法を用いた細胞内小胞輸送経路の解明 (2014)
  • Author(s): 豊岡公徳
  • Organizer: 医学生物学電子顕微鏡技術学会 第30回学術講演会
  • Place of Presentation: 大阪大学吹田キャンパス・銀杏会館
  • Year and Date: 2014-05-23 – 2014-05-25
  • Invited
• [Presentation] 広域TEM像取得システムと高圧凍結技法を用いた細胞内輸送系膜区画の超微形態解析 (2014)
  • Author(s): 豊岡公徳、佐藤繭子、朽名夏麿、若崎眞由美、澤木史江、桧垣匠、馳澤盛一郎、永田典子、松岡健
  • Organizer: 日本顕微鏡学会第70回学術講演会
  • Place of Presentation: 幕張メッセ
  • Year and Date: 2014-05-11 – 2014-05-13
• [Presentation] 細胞増殖期および定常状態期における分泌系オルガネラの電顕アトラス解析 (2014)
  • Author(s): 豊岡公徳、佐藤繭子、後藤友美、若崎眞由美、朽名夏麿、澤木史江、桧垣匠、馳澤盛一郎、永田典子、松岡健
  • Organizer: 日本植物生理学会年会、第55回日本植物生理学会年会
  • Place of Presentation: 富山大学、富山、日本
• [Presentation] 電顕アトラス法を用いたシロイヌナズナ根端組織におけるER body様構造体の分布と超微形態解析 (2014)
  • Author(s): 橋本恵、成川苗子、佐藤繭子、若崎眞由美、岡本龍史、豊岡公徳
  • Organizer: 日本植物生理学会年会、第55回日本植物生理学会年会
  • Place of Presentation: 富山大学、富山、日本
• [Presentation] 透過電顕像広域自動撮影システムの開発と植物組織への応用 (2014)
  • Author(s): 佐藤繭子、朽名夏麿、澤木史江、若崎眞由美、橋本恵、桧垣匠、馳澤盛一郎、永田典子、豊岡公徳
  • Organizer: 日本植物生理学会年会、第55回日本植物生理学会年会
  • Place of Presentation: 富山大学、富山、日本
• [Presentation] シロイヌナズナ根端組織におけるER body様構造体の分布と超微形態解析 (2014)
  • Author(s): 橋本恵、成川苗子、佐藤繭子、若崎眞由美、岡本龍史、豊岡公徳
  • Organizer: 日本顕微鏡学会、日本顕微鏡学会第３８回関東支部講演会
  • Place of Presentation: 日本女子大学、東京、日本
• [Presentation] 電顕アトラスの構築 : 高圧凍結技法を取り入れた広域電顕像撮影システムの開発 (2013)
  • Author(s): 佐藤繭子、朽名夏麿、若崎眞由美、澤木史江、桧垣匠、吉田拓弘、櫻井哲也、持田恵一、永田典子、豊岡公徳
  • Organizer: 日本植物生理学会
  • Place of Presentation: 岡山大学(岡山市)
  • Year and Date: 2013-03-21
• [Presentation] 高圧凍結技法を取り入れた広域透過電顕像撮影システムの開発とその応用 (2013)
  • Author(s): 豊岡公徳、佐藤繭子、朽名夏麿、永田典子
  • Organizer: 日本植物学会、日本植物学会第77回大会
  • Place of Presentation: 北海道大学、札幌、日本
  • Invited
• [Presentation] 電子顕微鏡法による新規細胞内小胞輸送経路の解明 (2013)
  • Author(s): 豊岡公徳
  • Organizer: 公益法人風戸研究奨励会、日本顕微鏡学会第６９回学術講演会
  • Place of Presentation: ホテル阪急エキスポパーク、大阪、日本
  • Invited
• [Presentation] 生体顕微マルチスケーリングマッピングシステム : 小胞クラスターの分布と構造 (2012)
  • Author(s): 豊岡公徳、若崎眞由美、朽名夏麿、桧垣匠、永田典子、松岡健、持田恵一、篠崎一雄、佐藤繭子
  • Organizer: 日本顕微鏡学会
  • Place of Presentation: つくば国際会議場(つくば市)
  • Year and Date: 2012-05-14
• [Remarks] 理化学研究所 環境資源科学研究センター 技術基盤部門 質量分析・顕微鏡解析ユニット 顕微鏡解析室
  • URL: http://bioem.riken.jp
• [Remarks] How plant cell compartments change
  • URL: http://phys.org/news/2014-08-cell-compartments-growth.html
• [Remarks] 省力・低コストで電子顕微鏡画像からオルガネラを検出する方法を開発
  • URL: http://www.k.u-tokyo.ac.jp/info/entry/7_entry24/
• [Remarks] 理化学研究所 環境資源科学研究センター 機能開発研究グループ 顕微鏡 ホームページ
  • URL: http://bioem.riken.jp
• [Remarks] 理化学研究所 環境資源科学研究センター 機能開発研究グループ ホームページ
  • URL: http://www.csrs.riken.jp/jp/labs/gdrg/index.html
• [Remarks]
  • URL: http://labs.psc.riken.jp/gdrg/Researchl/PSC_bioimaging/Top.html