| Budget Amount *help |
¥27,430,000 (Direct Cost: ¥21,100,000、Indirect Cost: ¥6,330,000)
Fiscal Year 2015: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2014: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2012: ¥10,660,000 (Direct Cost: ¥8,200,000、Indirect Cost: ¥2,460,000)
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| Outline of Final Research Achievements |
This study aimed to understand the neural pathway from sensory input (photoreceptor cells) to motor output (motor neurons and tail muscle) at the single cell level. To achieve this aim, I adopted innovative technology, such as transposon mediated transgenesis technique, transsynaptic labeling of specific neurons, live imaging of neuron activity and optogenetics for analyzing neuronal function. Here we showed that photoreceptor cells input into a few 2nd order neurons located in the posterior brain and the 2nd order neurons input into motor system that regulate swimming locomotion of the ascidian larva. We also found that novel gene regulatory mechanisms underlying photoreceptor specific gene expression and novel differentiation mechanisms of photoreceptor cells in ascidian embryo.
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