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Genetic approach to the neural pathway mediating the photic behavior of the ascidian larva: from senrory input to motor output

Research Project

Project/Area Number 24687008
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypePartial Multi-year Fund
Research Field Animal physiology/Animal behavior
Research InstitutionUniversity of Tsukuba

Principal Investigator

HORIE TAKEO  筑波大学, 生命環境系, 助教 (10455925)

Project Period (FY) 2012-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥27,430,000 (Direct Cost: ¥21,100,000、Indirect Cost: ¥6,330,000)
Fiscal Year 2015: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2014: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2012: ¥10,660,000 (Direct Cost: ¥8,200,000、Indirect Cost: ¥2,460,000)
Keywordsホヤ / 神経回路 / 光受容細胞 / 行動 / 光遺伝学
Outline of Final Research Achievements

This study aimed to understand the neural pathway from sensory input (photoreceptor cells) to motor output (motor neurons and tail muscle) at the single cell level. To achieve this aim, I adopted innovative technology, such as transposon mediated transgenesis technique, transsynaptic labeling of specific neurons, live imaging of neuron activity and optogenetics for analyzing neuronal function. Here we showed that photoreceptor cells input into a few 2nd order neurons located in the posterior brain and the 2nd order neurons input into motor system that regulate swimming locomotion of the ascidian larva. We also found that novel gene regulatory mechanisms underlying photoreceptor specific gene expression and novel differentiation mechanisms of photoreceptor cells in ascidian embryo.

Report

(6 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • Research Products

    (21 results)

All 2016 2015 2014 2013 2012 Other

All Int'l Joint Research (2 results) Journal Article (7 results) (of which Peer Reviewed: 7 results,  Acknowledgement Compliant: 1 results,  Open Access: 1 results) Presentation (10 results) (of which Int'l Joint Research: 3 results,  Invited: 5 results) Book (1 results) Remarks (1 results)

  • [Int'l Joint Research] プリンストン大学(米国)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] プリンストン大学(米国)

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Neuronal map reveals the highly regionalized pattern of the juvenile central nervous system of the ascidian Ciona intestinalis2015

    • Author(s)
      Hozumi A, Horie T, Sasakura Y
    • Journal Title

      Developmental Dynamics

      Volume: 244 Issue: 11 Pages: 1375-1393

    • DOI

      10.1002/dvdy.24317

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Nonreproductive role of gonadotropin-releasing hormone in the control of ascidian metamorphosis.2014

    • Author(s)
      Kamiya C, Ohta N, Ogura Y, Yoshida K, Horie T, Kusakabe TG, Satake H, Sasakura Y.
    • Journal Title

      Developmental Dynamics

      Volume: 243 Issue: 12 Pages: 1524-1535

    • DOI

      10.1002/dvdy.24176

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Enhancer activity sensitive to the orientation of the gene it regulates in the chordate genome2013

    • Author(s)
      Akiko Hozumi
    • Journal Title

      Developmental Biology

      Volume: 375 Issue: 1 Pages: 79-91

    • DOI

      10.1016/j.ydbio.2012.12.012

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cell lineage and cis-regulation for a unique GABAergic/glycinergic neuron type in the larval nerve cord of the ascidian Ciona intestinalis2012

    • Author(s)
      K.Nishitsuii
    • Journal Title

      Development, Growth and Differentiation

      Volume: 54 Issue: 2 Pages: 177-186

    • DOI

      10.1111/j.1440-169x.2011.01319.x

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Ascidians as excellent chordate models for studying the development of the nervous system during embryogenesis and metamorphosis.2012

    • Author(s)
      Yasunori Sasakura
    • Journal Title

      Developmental Growth and Differentiation

      Volume: 54 Issue: 3 Pages: 420-437

    • DOI

      10.1111/j.1440-169x.2012.01343.x

    • NAID

      10031136914

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Retinoic acid-driven Hoxl is required in the epidermis for forming the otic/atrial placodes during ascidian metamorphosis.2012

    • Author(s)
      Yasunori Sasakura
    • Journal Title

      Development

      Volume: (印刷中) Issue: 12 Pages: 2156-2160

    • DOI

      10.1242/dev.080234

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Monoaminergic modulation of photoreception in ascidian : Evidence for a proto-hypothalamo-retinal territory2012

    • Author(s)
      Florian Razy-Krajka, Euan R. Brown, Takeo Horie, Jacques Callebert, Yasunori Sasakura, Jean-Stéphane Joly, Takehiro G. Kusakabe, and Philippe Vernier
    • Journal Title

      BMC Biology

      Volume: 10 Issue: 1 Pages: 45-45

    • DOI

      10.1186/1741-7007-10-45

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Presentation] Specification of the lateral border of the neural plate in the ascidian embryo2016

    • Author(s)
      Ryoko Horie, Michael Levine, Takeo Horie
    • Organizer
      The 22nd International Congress of Zoology
    • Place of Presentation
      Okinawa Convention Center, Ginowan, Okinawa, Japan
    • Year and Date
      2016-11-17
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Calcium imaging and single cell optogenetic analysis of a neural circuit for generating swimming2016

    • Author(s)
      Takeo Horie, Masamichi Ohkura, Kotaro Shimai, Ryoko Horie, Yasunori Sasakura, Takehiro Kusakabe, Junichi Nakai, Michael Levine, Masashi Nakagawa
    • Organizer
      The 22nd International Congress of Zoology
    • Place of Presentation
      Okinawa Institute of Science and Technology,Onna-son, Okinawa, Japan
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Structural and physiological analyses of a neural circuit for swimming locomotion of the Ciona intestinalis2015

    • Author(s)
      Takeo Horie, Masamichi Ohkura, Kotaro Simai, Yasunori Sasakura, Takehiro Kusakabe, Junichi Nakai, Michael Levine, Masashi Nakagawa
    • Organizer
      The 8th International Tunicate Meeting
    • Place of Presentation
      Aomori City Cultural Hall, Aomori, Japan
    • Year and Date
      2015-07-13
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] ホヤ幼生の遊泳運動神経回路の構造と生理機能の解析2014

    • Author(s)
      堀江健生、大倉正道、日下部岳広、中井淳一、中川将司
    • Organizer
      日本動物学会第85回仙台大会
    • Place of Presentation
      東北大学川内キャンパス(宮城県仙台市)
    • Year and Date
      2014-09-11 – 2014-09-13
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Structural and physiological analysis of neural circuit for swimming locomotion of the ascidian larva2014

    • Author(s)
      Takeo Horie, Masamichi Ohkura, Yasunori Sasakura, Takehiro G Kusakabe4 Junichi Nakai, Masashi Nakagawa
    • Organizer
      Decoding principle of neural circuits using model animals II -
    • Place of Presentation
      名古屋大学 東山キャンパス(愛知県名古屋市)
    • Year and Date
      2014-09-09 – 2014-09-10
    • Related Report
      2014 Annual Research Report
  • [Presentation] Structural and physiological analysis for a central pattern generator controlling swimming locomotion of the ascidian larva2014

    • Author(s)
      Horie T
    • Organizer
      第91回日本生理学会大会
    • Place of Presentation
      鹿児島大学・群元キャンパス、鹿児島県
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Ependymal cells of chordate larvae are stem-like cells that form the adult nervous system2013

    • Author(s)
      Horie T
    • Organizer
      1^<st>JAMBIO International Symposium
    • Place of Presentation
      東京, 筑波大学.東京キャンパス(招待講演)
    • Year and Date
      2013-02-26
    • Related Report
      2012 Annual Research Report
  • [Presentation] In vivo calcium imaging of neural circuit for swimming locomotion of the ascidian larva2013

    • Author(s)
      Horie T
    • Organizer
      日本比較生理生化学会 第35回大会
    • Place of Presentation
      イーグレ姫路、兵庫県
    • Related Report
      2013 Annual Research Report
  • [Presentation] ホヤ幼生における遊泳運動神経回路の発生とその動作原理2013

    • Author(s)
      堀江 健生
    • Organizer
      第7回 Motor Control研究会ワークショップ
    • Place of Presentation
      東京大学農学部 弥生講堂 一条ホール・アネックス、東京都
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] In vivo calcium imaging of neural circuit for swimming locomotion of the ascidian larva2013

    • Author(s)
      Horie T
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸国際会議場、兵庫県
    • Related Report
      2013 Annual Research Report
  • [Book] 研究者が教える動物飼育第3巻「カタユウレイボヤの飼育」2012

    • Author(s)
      堀江健生、笹倉靖徳
    • Total Pages
      6
    • Publisher
      共立出版
    • Related Report
      2012 Annual Research Report
  • [Remarks] 筑波大学生命環境系下田臨海実験センター・堀江研究室ホームページ

    • Related Report
      2016 Annual Research Report

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Published: 2012-04-24   Modified: 2024-03-04  

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