The molecular basis of the functional retinal circuitry
| Project/Area Number |
24687010
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Partial Multi-year Fund |
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| Research Field |
Animal physiology/Animal behavior
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
Onishi Akishi 国立研究開発法人理化学研究所, 多細胞システム形成研究センター, 研究員 (70569102)
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| Project Period (FY) |
2012-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥27,560,000 (Direct Cost: ¥21,200,000、Indirect Cost: ¥6,360,000)
Fiscal Year 2014: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
Fiscal Year 2013: ¥9,620,000 (Direct Cost: ¥7,400,000、Indirect Cost: ¥2,220,000)
Fiscal Year 2012: ¥10,790,000 (Direct Cost: ¥8,300,000、Indirect Cost: ¥2,490,000)
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| Keywords | 網膜 / 視細胞 / 中心窩 / 神経回路 / 視覚 / 神経回路形成 |
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| Outline of Final Research Achievements |
In this study, we performed functional screening of molecules that govern specific synaptic connection between photoreceptors (rod, S cone and M cone) and secondary neurons (horizontal cell and bipolar cells) in the mouse retina. When we electroporated Susd3 cDNA, a single transmembrane protein specifically expressed in rod photoreceptors in the mouse retinas, the photoreceptors showed branching synaptic terminals that targeted secondary neurons in a dose dependent manner. Next, we developed double knockout mice of Aldh1a1 and Cyp1b1, enzymes that synthesize ligands for Rxrg, and observed significant decrease of M cones. In addition, Aldh1a1 deficient mice showed choroidal hypoplasia.
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Report
(5 results)
Research Products
(10 results)
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[Journal Article] Optimized Culture System to Induce Neurite Outgrowth From Retinal Ganglion Cells in Three-Dimensional Retinal Aggregates Differentiated From Mouse and Human Embryonic Stem Cells.2016
Author(s)
Maekawa Y, Onishi A, Matsushita K, Koide N, Mandai M, Suzuma K, Kitaoka T, Kuwahara A, Ozone C, Nakano T, Eiraku M, Takahashi M
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Journal Title
Curr Eye Res
Volume: 19
Pages: 1-11
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] Neurite outgrowth from retinal ganglion cells in self-organizing mouse and human retinal tissues differentiated from embryonic stem cells2015
Author(s)
Maekawa Y, Onishi A, Matsushita K, Koide N, Mandai M, Suzuma K, Kitaoka T, Kuwahara A, Ozone C, Nakano T, Eiraku M, Takahashi M.
Organizer
The International Society for Stem Cell Research
Place of Presentation
スウェーデン ストックホルム
Year and Date
2015-06-24
Related Report
Int'l Joint Research
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