| Budget Amount *help |
¥27,430,000 (Direct Cost: ¥21,100,000、Indirect Cost: ¥6,330,000)
Fiscal Year 2014: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
Fiscal Year 2013: ¥10,400,000 (Direct Cost: ¥8,000,000、Indirect Cost: ¥2,400,000)
Fiscal Year 2012: ¥9,880,000 (Direct Cost: ¥7,600,000、Indirect Cost: ¥2,280,000)
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| Outline of Final Research Achievements |
Cultured enrichments and isolates are important in developing our understanding of microbial physiology, genetics, and ecology. However, many microorganisms, particularly members of the domain Archaea, remain uncultured under laboratory conditions. In this study, I attempted to cultivate and isolate uncultured Archaea using a new cultivation technique I developed and molecular techniques. As a result, I successfully obtained three anaerobic methane-oxidizing microbial communities that contain phylogenetically diverse of uncultured archaeal groups. The single cell genome analysis for one of the enriched microbial communities revealed that an uncultured archaeal group might have a unique sulfur metabolism gene. Unfortunately, isolates of the uncultured archaeal group was not obtained during the period of this study, but the enrichments and single cell genome analysis provide new information about the uncultured archaea.
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