| Budget Amount *help |
¥27,430,000 (Direct Cost: ¥21,100,000、Indirect Cost: ¥6,330,000)
Fiscal Year 2015: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2014: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2013: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2012: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000)
|
|---|
| Outline of Final Research Achievements |
The aim of this study was to determine the crystal structure of the ubiquitin (Ub) ligase complex LUBAC that specifically synthesizes Met1-linked Ub chain. However, the structure of LUBAC in complex with Ub was determined by the group of Katrin Rittinger in 2013, and therefore we had to change our research theme.
On the other hand, we purified several kinds of enzymes for Ub chain synthesis in the process of LUBAC research. With these enzymes, we succeeded in large-scale preparation of Lys6-, Lys29- and Lys33-linked Ub chains which were difficult to be obtained. In addition, we determined the crystal structures of CYLD in complex with Met1-linked diUb, CYLD in complex with Lys63-linked diUb, FAAP20 in complex with Lys63-linked diUb and USP30 in complex with Lys 6-linked diUb.
|
