Structure and mechanism of the iron transport system in human
| Project/Area Number |
24687015
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Partial Multi-year Fund |
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| Research Field |
Structural biochemistry
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
Sugimoto Hiroshi 国立研究開発法人理化学研究所, 放射光科学総合研究センター, 専任研究員 (90344043)
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| Research Collaborator |
Hiromi Togashi
Takeshi Urano
Nami Yashima
Hanae Takeda
Mauk Grant A.
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| Project Period (FY) |
2012-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥27,300,000 (Direct Cost: ¥21,000,000、Indirect Cost: ¥6,300,000)
Fiscal Year 2015: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2014: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2013: ¥13,260,000 (Direct Cost: ¥10,200,000、Indirect Cost: ¥3,060,000)
Fiscal Year 2012: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
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| Keywords | 金属タンパク質 / 鉄イオン / 金属イオン / ヘム / トランスポーター / X線 / 結晶 |
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| Outline of Final Research Achievements |
We performed the structural and functional analysis of transmembrane ferrireductase expressed in human duodenal brush border membrane. This enzyme contains two hemes as a cofactor to mediate the electron transfer from intracellular ascorbic acid (Asc) to ferric iron in the intestinal lumen. Our stopped-flow kinetic analysis of the ferrireductase and its mutants in the detergent solution suggested that the conserved positively charged residues are involved in Asc-binding. We obtained the crystals by the lipidic cubic phase method and determined the structure of ferrireductase.
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Report
(5 results)
Research Products
(17 results)
