Elucidation of the Function of Vaults based on its whole structure.
| Project/Area Number |
24687020
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Partial Multi-year Fund |
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| Research Field |
Biophysics
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| Research Institution | Osaka University |
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Principal Investigator |
TANAKA Hideaki 大阪大学, たんぱく質研究所, 准教授 (40346169)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥27,820,000 (Direct Cost: ¥21,400,000、Indirect Cost: ¥6,420,000)
Fiscal Year 2014: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
Fiscal Year 2012: ¥14,560,000 (Direct Cost: ¥11,200,000、Indirect Cost: ¥3,360,000)
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| Keywords | ボルト / 脂質ラフト / 自然免疫 / X線結晶構造解析 |
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| Outline of Final Research Achievements |
Vault is the largest cytoplasmic nucleoprotein particle. However we have determined the x-ray structure of rat liver vault at 3.5 angstrom resolution in 2009 (Science 323 384-288, 2009), the electron density map around the waist domain (N terminal domain of MVP) was disordered. Thus we added leucine zipper to the N-terminus of MVP (LZ-MVP), and expressed LZ-MVP in the Sf9 insect cell line using a baculovirus vector. We obtained more stable vault particles (LZ-vaults) in high purity and yield (80mg LZ-vaults/1L culture). The crystals of LZ vaults are diffracted up to 2.8angstrom resolution on beam line BL44XU at SPring-8.
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Report
(4 results)
Research Products
(10 results)
