Analysis of cell division cycle regulated by PKase-PPase combination

• Project/Area Number: 24687025
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Partial Multi-year Fund
• Research Field: Cell biology
• Research Institution: Kumamoto University
• Principal Investigator: Mochida Satoru 熊本大学, 大学院先導機構, 准教授 (60590304)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥27,430,000 (Direct Cost: ¥21,100,000、Indirect Cost: ¥6,330,000); Fiscal Year 2014: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000); Fiscal Year 2013: ¥8,450,000 (Direct Cost: ¥6,500,000、Indirect Cost: ¥1,950,000); Fiscal Year 2012: ¥10,660,000 (Direct Cost: ¥8,200,000、Indirect Cost: ¥2,460,000)
• Keywords: 細胞分裂周期 / タンパク質リン酸化 / リン酸化 / 脱リン酸化酵素 / 細胞周期 / 細胞分裂

Outline of Final Research Achievements

This project aimed to reveal, by using bichemical and cell biological approaches, how protein phosphorylation important for driving cell division is regulated. Especially we focused on ENSA protein, a linkage of protein kinase and phosphatase, that the appilicant recently discovered.

Research Products

• [Journal Article] Cyclin B-Cdk1 inhibits protein phosphatase PP2A-B55 via a Greatwall kinase-independent mechanism. (2014)
  • Author(s): Okumura, E., Morita, A., Wakai, M., Mochida, S., Hara, M., and Kishimoto, T.
  • Journal Title: J. Cell Biol. Volume: 204 Issue: 6 Pages: 881-889
  • DOI: 10.1083/jcb.201307160
  • Peer Reviewed
• [Journal Article] Regulation of α-endosulfine, an inhibitor of protein phosphatase 2A, by multisite phosphorylation. (2014)
  • Author(s): Mochida S.
  • Journal Title: FEBS Journal Volume: 281 Issue: 4 Pages: 1159-1169
  • DOI: 10.1111/febs.12685
  • Peer Reviewed
• [Journal Article] キナーゼとホスファターゼの連係による細胞周期制御 (2013)
  • Author(s): 持田 悟
  • Journal Title: 細胞工学 Volume: 32 Pages: 309-313
• [Presentation] リン酸化の可逆性が生み出す 細胞分裂周期の制御 (2014)
  • Author(s): 持田悟
  • Organizer: 認識と形成2014
  • Place of Presentation: 熊本 同仁堂ホール
  • Year and Date: 2014-09-14 – 2014-09-15
  • Invited
• [Presentation] Regulation of alpha-endosulfine, an inhibitor of PP2A-B55, (2013)
  • Author(s): Mochida S.
  • Organizer: The 2nd Taiwan-Japan Bilateral Conference on Protein Phosphatases
  • Place of Presentation: 台湾 新竹
  • Invited
• [Remarks] 研究室HP
  • URL: http://cellcyclecontrol.jp
• [Remarks]
  • URL: http://www.cellcyclecontrol.jp