Regulation of axial stem cells deriving neural plate cells and mesoderm cells

• Project/Area Number: 24687029
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Partial Multi-year Fund
• Research Field: Developmental biology
• Research Institution: The University of Tokushima (2013-2015); Osaka University (2012)
• Principal Investigator: TKEMOTO Tatsuya 徳島大学, 藤井節郎記念医科学センター, 助教 (30443899)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥27,430,000 (Direct Cost: ¥21,100,000、Indirect Cost: ¥6,330,000); Fiscal Year 2014: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000); Fiscal Year 2013: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000); Fiscal Year 2012: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000)
• Keywords: 細胞運命 / 転写制御 / 細胞分化 / Wntシグナル / 体軸幹細胞

Outline of Final Research Achievements

1. I have studied the relationship between the intensity of Wnt signal and the cell fate of axial stem cells.
2. I have identified the culture condition to derive axial stem cells from epiblast stem cells, and analyzed the difference of expression patterns.
3. I have established a new method to generate genetically modified mouse using CRISPR/Cas9 system.

Research Products

• [Journal Article] R26-WntVis reporter mice showing graded response to Wnt signal levels. (2016)
  • Author(s): Takemoto T, Abe T, Kiyonari H, Nakao K, Furuta Y, Suzuki H, Takada S, Fujimori T, Kondoh H.
  • Journal Title: Genes Cells. Volume: 印刷中 Issue: 6 Pages: 661-669
  • DOI: 10.1111/gtc.12364
  • NAID: 120006306641
  • Peer Reviewed / Open Access
• [Journal Article] Electroporation enables the efficient mRNA delivery into the mouse zygotes and facilitates CRISPR/Cas9-based genome editing. (2015)
  • Author(s): Hashimoto M. and Takemoto T.
  • Journal Title: Scientific Reports Volume: 5 Issue: 1 Pages: 11315-11315
  • DOI: 10.1038/srep11315
  • NAID: 120006875680
  • Peer Reviewed / Open Access
• [Journal Article] Regulation of the axial stem cells deriving neural tube and paraxial mesoderm (2014)
  • Author(s): 竹本龍也
  • Journal Title: Leading Author's Volume: 3 Pages: e007
  • DOI: 10.7875/leading.author.3.e007
  • Open Access
• [Journal Article] Lovell-Badge R, Takemoto T, Kondoh H. Regulation of mesodermal precursor production by low-level expression of B1 Sox genes in the caudal lateral epiblast (2014)
  • Author(s): Yoshida M, Uchikawa M, Rizzoti K
  • Journal Title: Mech Dev Volume: 132 Pages: 59-68
  • DOI: 10.1016/j.mod.2014.01.003
  • Peer Reviewed
• [Journal Article] Mechanism of cell fate choice between neural and mesodermal development during early embryogenesis (2013)
  • Author(s): Tatsuya Takemoto
  • Journal Title: Congenital Anomalies Volume: 53(2) Issue: 2 Pages: 61-66
  • DOI: 10.1111/cga.12017
  • Peer Reviewed
• [Presentation] High-throughput production of mutant mice by electroporation of CRISPR/Cas9 system (2015)
  • Author(s): Tatsuya Takemoto, Masakazu Hashimoto
  • Organizer: Mouse Molecular Genetics 2015
  • Place of Presentation: Cambridge (UK)
  • Year and Date: 2015-09-17
  • Int'l Joint Research
• [Presentation] High-throughput production of mutant mice by electroporation of CRISPR/Cas9 system (2015)
  • Author(s): Tatsuya Takemoto, Masakazu Hashimoto
  • Organizer: the 48th Annual Meeting of the Japanese Society of Developmental Biologists
  • Place of Presentation: つくば国際会議場（茨城県・つくば市）
  • Year and Date: 2015-06-03
  • Int'l Joint Research
• [Presentation] The role of Tbx6 in the derivation of mesodermal tissue from the axial stem cells (2014)
  • Author(s): Takemoto T and Kondoh H.
  • Organizer: 47th Annual Meeting of the Japanese Society of Developmental Biologists
  • Place of Presentation: ウィンク愛知（愛知県・名古屋市）
  • Year and Date: 2014-05-28
  • Int'l Joint Research
• [Presentation] Regulation of Axial Stem Cells Deriving Neural Plate and Paraxial Mesoderm (2013)
  • Author(s): Tatsuya Takemoto
  • Organizer: The 61th NIBB conference: Cellular Community in Mammalian Embryogenesis
  • Place of Presentation: 岡崎カンファレンスセンター（名古屋市・愛知県）
  • Year and Date: 2013-06-10 – 2013-06-12
• [Presentation] Regulation of axial stem cells deriving neural and mesodermal tissues (2012)
  • Author(s): Tatsuya Takemoto
  • Organizer: Joint meeting of JSDB 45th & JSCB 64th
  • Place of Presentation: 神戸市
  • Invited
• [Presentation] The regulation of mesodermal tissue development from the axial stem cells by Tbx6L (2012)
  • Author(s): Tatsuya Takemoto
  • Organizer: 7th international chick meeting
  • Place of Presentation: 名古屋市
• [Presentation] Regulation of axial stem cells deriving neural and mesodermal tissues (2012)
  • Author(s): Tatsuya Takemoto
  • Organizer: Hong Kong Society for Developmental Biology Symposium
  • Place of Presentation: 香港（中国）
• [Book] New Principles in Developmental Processes (2014)
  • Author(s): Takemoto T.
  • Total Pages: 321
  • Publisher: Springer
• [Remarks] 徳島大学藤井節郎記念医科学センター・初期発生研究分野
  • URL: http://www.fujii.tokushima-u.ac.jp/embryology/
• [Remarks] 徳島大学藤井節郎記念医科学センター 初期発生研究分野ホームページ
  • URL: http://www.fujii.tokushima-u.ac.jp/embryology/
• [Remarks] 大阪大学大学院生命機能研究科 近藤寿人研究室ホームページ
  • URL: http://www.fbs.osaka-u.ac.jp/labs/kondoh/Kondoh_Lab/Welcome.html