| Budget Amount *help |
¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
Fiscal Year 2015: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2014: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2013: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2012: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
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| Outline of Final Research Achievements |
We designed and developed an incubator for 15N-gas exposure experiments with a domestic manufacturer. Mice with or without nitrogen-fixing bacteria were kept for 3 days under 15N gas in the incubator. The internal organs (intestinal contents, intestinal tract, liver, body hair) of the mice were harvested and analyzed nitrogen Isotope ratio of the samples using an elemental analysis / isotope ratio mass spectrometer to measure. The results indicated that a statistically significant increase in the nitrogen isotope ratio was found in intestinal content, intestinal tract, and liver. In contrast, no significance was observed in the body hair.
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