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Molecular mechanisms for formation of florigen activation complex and its application toward flowering control

Research Project

Project/Area Number 24688001
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypePartial Multi-year Fund
Research Field Breeding science
Research InstitutionNara Institute of Science and Technology

Principal Investigator

TSUJI Hiroyuki  奈良先端科学技術大学院大学, バイオサイエンス研究科, 助教 (40437512)

Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥25,090,000 (Direct Cost: ¥19,300,000、Indirect Cost: ¥5,790,000)
Fiscal Year 2014: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
Fiscal Year 2013: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
Fiscal Year 2012: ¥9,620,000 (Direct Cost: ¥7,400,000、Indirect Cost: ¥2,220,000)
Keywordsフロリゲン / メリステム / イネ / 花成 / フロリゲン活性化複合体 / 14-3-3タンパク質 / FD
Outline of Final Research Achievements

Florigen is a systemic floral signal that is generated in leaves and transported to the shoot apical meristem (SAM). The molecular nature of florigen is elucidated recently as the FT/Hd3a protein, and florigen receptor and functional transcriptional complex are identified. However, key questions regarding florigen complex remain to be answered. They include distribution of florigen and formation of the complex in the SAM. In this study these questions are answered through the unique technique of imaging SAM. The results indicated that florigen distributes throughout the course of inflorescence development to regulate its architecture. Molecular basis of multi-functionality of florigen is also revealed. The transcription factors (TFs) incorporated into the florigen complex determine the functional consequence of the resultant complex, and coincidence of florigen distribution and organ-specific expression of TFs detemine the different functions in the different developmental contexts.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • Research Products

    (27 results)

All 2015 2014 2013 2012 Other

All Journal Article (10 results) (of which Peer Reviewed: 10 results,  Open Access: 1 results) Presentation (16 results) (of which Invited: 7 results) Book (1 results)

  • [Journal Article] FT-like proteins induce transposon silencing in the shoot apex during floral induction in rice2015

    • Author(s)
      Shojiro Tamaki, Hiroyuki Tsuji, Ayana Matsumoto, Akiko Fujita, Zenpei Shimatani, Rie Terada, Tomoaki, Sakamoto, Tetsuya Kurata and Ko Shimamoto
    • Journal Title

      Proceedings of the National Academy of Sciences

      Volume: 112 Issue: 8

    • DOI

      10.1073/pnas.1417623112

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Hd3a promotes lateral branching in rice.2015

    • Author(s)
      Tsuji H, Tachibana C, Tamaki S, Taoka KI, Kyozuka J, Shimamoto K.
    • Journal Title

      Plant J.

      Volume: 82 Issue: 2 Pages: 256-266

    • DOI

      10.1111/tpj.12811

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Florigen in rice : complex gene network for florigen transcription, florigen activation complex, and multiple functions2013

    • Author(s)
      辻 寛之、田岡 健一郎、島本 功
    • Journal Title

      Curr. Opin. Plant Biol

      Volume: 16 Issue: 2 Pages: 228-235

    • DOI

      10.1016/j.pbi.2013.01.005

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Structure and function of florigen and the receptor complex2013

    • Author(s)
      田岡 健一郎、大木 出、辻 寛之、児嶋 長次郎、島本 功
    • Journal Title

      Trends Plant Sci

      Volume: 18 Issue: 5 Pages: 287-294

    • DOI

      10.1016/j.tplants.2013.02.002

    • Related Report
      2013 Annual Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Structure and function of flowering hormone "florigen"2013

    • Author(s)
      辻 寛之、田岡健一郎、島本 功
    • Journal Title

      Leading Author's

      Volume: 2 Pages: e004

    • DOI

      10.7875/leading.author.2.e004

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Functional diversification of FD transcription factors in rice, components of florigen activation complexes2013

    • Author(s)
      辻 寛之、中村 洋之、田岡 健一郎、島本 功
    • Journal Title

      Plant Cell Physiol

      Volume: 54 Issue: 3 Pages: 385-397

    • DOI

      10.1093/pcp/pct005

    • NAID

      10031163834

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Flowering in rice.2013

    • Author(s)
      Tsuji H, Shimamoto K
    • Journal Title

      Genetics and Genomics of Rice (eds. Qifa Zhang and Rod Wing),

      Volume: (In press)

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] フロリゲンが花を咲かせるメカニズム2012

    • Author(s)
      辻 寛之, 島本 功
    • Journal Title

      農業技術大系 花卉編

      Volume: 32 Pages: 2-9

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 花咲ホルモン (フロリゲン)2012

    • Author(s)
      辻 寛之, 田岡健一郎、島本 功
    • Journal Title

      植調

      Volume: 46 Pages: 179-186

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 花成ホルモンフロリゲンとその受容体の構造解析からみえてきたフロリゲン機能の分子基盤.2012

    • Author(s)
      田岡健一郎, 大木 出, 辻 寛之, 児嶋長次郎, 島本 功
    • Journal Title

      化学と生物

      Volume: 50 Pages: 654-659

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Presentation] (奨励賞受賞講演)花成ホルモン・フロリゲンの機能に関する遺伝育種学的研究2015

    • Author(s)
      辻 寛之
    • Organizer
      日本育種学会
    • Place of Presentation
      玉川大学(東京都町田市)
    • Year and Date
      2015-03-21
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Carrying the ball for flowering: Florigen distribution and epigenomic regulation in the shoot apical meristem2015

    • Author(s)
      Hiroyuki Tsuji
    • Organizer
      日本植物生理学会
    • Place of Presentation
      東京農業大学(東京都世田谷区)
    • Year and Date
      2015-03-17
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Coordination of flowering and gene expression by rice Hd3a florigen2014

    • Author(s)
      Hiroyuki Tsuji
    • Organizer
      International Symposium on Rice Functional Genomics 2014
    • Place of Presentation
      Tucson, Arizona, USA
    • Year and Date
      2014-10-17
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] The flowering hormone florigen: its structure, receptor complex, and beyond2014

    • Author(s)
      Hiroyuki Tsuji
    • Organizer
      第38回 内藤コンファレンス
    • Place of Presentation
      北海道札幌市
    • Year and Date
      2014-10-09
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] The flowering hormone florigen: its structure, receptor complex, and beyond2014

    • Author(s)
      Tsuji H.
    • Organizer
      Plant and Animal Genome (PAG) XXII
    • Place of Presentation
      San Diego, USA
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] イネのフロリゲンHd3aと相互作用する転写因子OsFDの機能解析2013

    • Author(s)
      辻 寛之、中村 洋之、玉置 祥二郎、田岡 健一郎、島本 功
    • Organizer
      第54回日本植物生理学会講演会
    • Place of Presentation
      岡山県岡山市
    • Year and Date
      2013-03-21
    • Related Report
      2012 Annual Research Report
  • [Presentation] Molecular mechanism of florigen function and its2013

    • Author(s)
      Tsuji H.
    • Organizer
      Japan-China Joint Symposium on Rice Developmental Biology
    • Place of Presentation
      大分県別府市
    • Year and Date
      2013-03-09
    • Related Report
      2012 Annual Research Report
  • [Presentation] フロリゲンの受容と機能のメカニズム2013

    • Author(s)
      辻 寛之
    • Organizer
      熊本県農業研究センター農産園芸研究所講演会
    • Place of Presentation
      熊本県合志市
    • Year and Date
      2013-03-06
    • Related Report
      2012 Annual Research Report
  • [Presentation] フロリゲンを中心とした転写活性化複合体FACの核移行メカニズムの解析2013

    • Author(s)
      中村洋之、辻寛之、島本功
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      兵庫県神戸市神戸国際展示場
    • Related Report
      2013 Annual Research Report
  • [Presentation] ジーンターゲティングにより作成したOsMADS15-mOrangeトランスジェニックイネの解析2012

    • Author(s)
      松本彩奈, 島谷善平, 辻寛之, 寺田理枝, 島本功
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡県福岡市
    • Year and Date
      2012-12-12
    • Related Report
      2012 Annual Research Report
  • [Presentation] 全ゲノムバイサルファイトシーケンス法による茎頂メリステムの生長相転換過程のメチローム解析2012

    • Author(s)
      才原 徳子, 辻 寛之, 三浦 史仁, 伊藤 隆司, 島本 功
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡県福岡市
    • Year and Date
      2012-12-12
    • Related Report
      2012 Annual Research Report
  • [Presentation] 14-3-3タンパク質は花成ホルモン・フロリゲンの細胞内受容体として機能する2012

    • Author(s)
      辻 寛之、田岡 健一郎、大木 出、玉置 祥二郎、中島 千佳、中村 洋之、稲田 のりこ、児嶋 長次郎、島本 功
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡県福岡市
    • Year and Date
      2012-12-12
    • Related Report
      2012 Annual Research Report
  • [Presentation] 花咲かじいさんの灰-取り扱い説明書2012

    • Author(s)
      辻 寛之
    • Organizer
      国立遺伝学研究所研究集会「イネ分子遺伝学の夢」
    • Place of Presentation
      静岡県三島市
    • Year and Date
      2012-11-16
    • Related Report
      2012 Annual Research Report
  • [Presentation] 花成ホルモン・フロリゲンの受容と機能のメカニズム2012

    • Author(s)
      辻寛之・中島千佳・田岡健一郎・大木出・児嶋長次郎・稲田のりこ・島本 功
    • Organizer
      日本遺伝学会第84回大会
    • Place of Presentation
      福岡県福岡市
    • Year and Date
      2012-09-25
    • Related Report
      2012 Annual Research Report
  • [Presentation] Molecular mechanism of florigen function and application of florigen to crop improvement

    • Author(s)
      Tsuji H.
    • Organizer
      CREST International Symposium; Productivity Improvement of Plants: From Model to Crop Plants
    • Place of Presentation
      奈良県奈良市東大寺文化ホール
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Molecular mechanism of florigen function and beyond

    • Author(s)
      Tsuji H.
    • Organizer
      8th Joint Korea-Japan Plant Biotech Workshop onNew Biotechnology-based Plant Breeding Techniques
    • Place of Presentation
      北海道札幌市北海道大学
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Book] Florigen signalling ( in The Enzymes)2014

    • Author(s)
      Tsuji H, Taoka K.
    • Total Pages
      30
    • Publisher
      Elsevier
    • Related Report
      2014 Annual Research Report

URL: 

Published: 2012-04-24   Modified: 2019-07-29  

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