| Budget Amount *help |
¥26,130,000 (Direct Cost: ¥20,100,000、Indirect Cost: ¥6,030,000)
Fiscal Year 2015: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2014: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2013: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
Fiscal Year 2012: ¥11,310,000 (Direct Cost: ¥8,700,000、Indirect Cost: ¥2,610,000)
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| Outline of Final Research Achievements |
This research object is to gain the knowledge about the mode of unreduced pollen formation of ‘Nishiuchi Konatsu’ Hyuganatsu and to improve the recovery rate of plantlet by the embryo rescue. Six heterozygotic SSR markers, which are located on the same chromosome in ‘Nishiuchi Konatsu’, were screened. Unreduced pollen grains were genotyped for these SSR loci by the single-pollen genotyping. The inheritance of heterozygosity implied the mode of unreduced pollen formation was occurred through first division restitution (FDR). For embryo rescue, it was revealed that gibberellic acid was effective only for the initial culture and the fruit of 40-50 mm in diameter were appropriate for the embryo rescue.
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