Cross talk between RNA silencing and plant innate immune responses to viruses

• Project/Area Number: 24688005
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Partial Multi-year Fund
• Research Field: Plant pathology
• Research Institution: The University of Tokyo
• Principal Investigator: YAMAJI YASUYUKI 東京大学, 農学生命科学研究科, 准教授 (40345187)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥24,830,000 (Direct Cost: ¥19,100,000、Indirect Cost: ¥5,730,000); Fiscal Year 2015: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2014: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2013: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000); Fiscal Year 2012: ¥9,230,000 (Direct Cost: ¥7,100,000、Indirect Cost: ¥2,130,000)
• Keywords: 植物・病原体相互作用 / RNAサイレンシング / 自然免疫応答 / 植物ウイルス / 抵抗性

Outline of Final Research Achievements

Plants combat with viruses using two defense mechanisms, RNA silencing and plant innate immunity. In this study, we analyzed the role of an RNA silencing suppressor, TGBp1. We found that TGBp1 interacted with Arabidopsis thaliana proteins SGS3 and RDR6, both of which are involved in double-stranded RNA synthesis, to aggregate and inactivate their activities in planta. We also analyzed the mechanism of JAX1-mediated resistance and revealed the a viral protein responsible for it. Based on these results, we elucidated various plant-virus molecular interactions.

Research Products

• [Journal Article] Complete genome sequences of two hydrangea ringspot virus isolates from Japan. (2016)
  • Author(s): Yusa, A., Iwabuchi, N., Koinuma, H., Keima, T., Neriya, Y., Hashimoto, M., Maejima, K., Yamaji, Y., Namba, S.
  • Journal Title: Genome Announc Volume: 4 Issue: 2
  • DOI: 10.1128/genomea.00022-16
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Complete genome sequence of Alternanthera mosaic virus, isolated from Achyranthes bidentata in Asia. (2016)
  • Author(s): Iwabuchi, N., Yoshida, T., Yusa, A., Nishida, S., Tanno, K., Keima, T., Nijo, T., Yamaji, Y., Namba, S.
  • Journal Title: Genome Announc Volume: 4 Issue: 2
  • DOI: 10.1128/genomea.00020-16
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Rapid detection of fig mosaic virus using reverse transcription loop-mediated isothermal amplification (2015)
  • Author(s): Kazuya Ishikawa, Kensaku Maejima, Osamu Netsu, Misato Fukuoka, Takamichi Nijo, Masayoshi Hashimoto, Daisuke Takata, Yasuyuki Yamaji, Shigetou Namba
  • Journal Title: Journal of General Plant Pathology Volume: 81 Issue: 5 Pages: 382-389
  • DOI: 10.1007/s10327-015-0603-1
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Cell death triggered by a putative amphipathic helix of radish mosaic virus helicase protein is tightly correlated with host membrane modification. (2015)
  • Author(s): Hashimoto M., Komatsu K., Iwai R., Keima T., Maejima K., Shiraishi T., Ishikawa K., Yoshida T., Kitazawa Y., Okano Y., Yamaji Y., Namba S.
  • Journal Title: Molecular Plant-Microbe Interactions Volume: 28 Issue: 6 Pages: 675-88
  • DOI: 10.1094/mpmi-01-15-0004-r
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Nucleocapsid protein from fig mosaic virus forms cytoplasmic agglomerates that are hauled by endoplasmic reticulum streaming. (2015)
  • Author(s): Ishikawa K., Miura C., Maejima K., Komatsu K., Hashimoto M., Tomomitsu T., Fukuoka M., Yusa A., Yamaji Y., Namba S.
  • Journal Title: Journal of Virology Volume: 89 Issue: 1 Pages: 480-491
  • DOI: 10.1128/jvi.02527-14
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] The phytoplasmal virulence factor TENGU causes plant sterility by downregulating of the jasmonic acid and auxin pathways. (2014)
  • Author(s): Minato, N., Himeno, M., Hoshi, A., Maejima, K., Komatsu, K., Takebayashi, Y., Kasahara, H., Yusa, A., Yamaji, Y., Oshima, K., Kamiya, Y. & Namba, S.
  • Journal Title: Scientific reports Volume: 4 Issue: 1 Pages: 1-7
  • DOI: 10.1038/srep07399
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Complete nucleotide sequence and genome structure of a Japanese isolate of hibiscus latent Fort Pierce virus, a unique tobamovirus that contains an internal poly(A) region in its 3' end (2014)
  • Author(s): Yoshida T., Kitazawa Y., Komatsu K., Neriya Y., Ishikawa K., Fujita N., Hashimoto M., Maejima K., Yamaji Y., Namba S
  • Journal Title: Archives of Virology Volume: 159 Issue: 11 Pages: 3161-3165
  • DOI: 10.1007/s00705-014-2175-3
  • Peer Reviewed
• [Journal Article] In Planta Recognition of a Double-Stranded RNA Synthesis Protein Complex by a Potexviral RNA Silencing Suppressor. (2014)
  • Author(s): Okano Y., Senshu H., Hashimoto M., Neriya Y., Netsu O., Minato N., Yoshida T., Maejima K., Oshima K., Komatsu K., Yamaji Y., Namba S.
  • Journal Title: Plant Cell Volume: 26 Issue: 5 Pages: 2168-2183
  • DOI: 10.1105/tpc.113.120535
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Recognition of floral homeotic MADS-domain transcription factors by a phytoplasmal effector, phyllogen, induces phyllody (2014)
  • Author(s): Maejima K., Iwai R., Himeno M., Komatsu K., Kitazawa Y., Fujita N., Ishikawa K., Fukuoka M., Minato N., Yamaji Y., Oshima K., Namba S
  • Journal Title: The Plant J Volume: 78 Issue: 4 Pages: 541-554
  • DOI: 10.1111/tpj.12495
  • Peer Reviewed / Open Access
• [Journal Article] Purple top symptoms are associated with reduction of leaf cell death in phytoplasma-infected plants. (2014)
  • Author(s): Himeno M., Kitazawa Y., Yoshida T., Maejima K., Yamaji Y., Oshima K., Namba S.
  • Journal Title: Scientific Reports Volume: 4 Issue: 1 Pages: 111-111
  • DOI: 10.1038/srep04111
  • Peer Reviewed / Open Access
• [Journal Article] Development of an on-site plum pox virus detection kit based on immunochromatography. (2014)
  • Author(s): Maejima K., Himeno M., Netsu O., Ishikawa K., Yoshida T., Fujita N., Hashimoto M., Komatsu K., Yamaji Y., Namba S.
  • Journal Title: Journal of General Plant Pathology Volume: 80 Issue: 2 Pages: 176-183
  • DOI: 10.1007/s10327-014-0504-8
  • Peer Reviewed
• [Journal Article] Efficient foreign gene expression in planta using a plantago asiatica mosaic virus-based vector achieved by the strong RNA-silencing suppressor activity of TGBp1. (2014)
  • Author(s): Minato N., Komatsu K., Okano Y., Maejima K., Ozeki J., Senshu H., Takahashi S., Yamaji Y., Namba S.
  • Journal Title: Archives of Virology Volume: (印刷中) Issue: 5 Pages: 885-896
  • DOI: 10.1007/s00705-013-1860-y
  • Peer Reviewed
• [Journal Article] First report of tomato chlorotic dwarf viroid isolated from symptomless petunia plants (Petunia spp.) in Japan. (2013)
  • Author(s): 白石 拓也
  • Journal Title: Journal of General Plnat Pathology Volume: (印刷中) Issue: 3 Pages: 214-216
  • DOI: 10.1007/s10327-013-0444-8
  • Peer Reviewed
• [Journal Article] Construction of an infectious cDNA clone of radish mosaic virus, a crucifer-infecting comovirus. (2013)
  • Author(s): 小松 健
  • Journal Title: Archives of Virology Volume: (印刷中) Issue: 7 Pages: 1579-1582
  • DOI: 10.1007/s00705-013-1635-5
  • Peer Reviewed
• [Journal Article] Fig mosaic emaravirus p4 protein is involved in cell-to-cell movement. (2013)
  • Author(s): 石川 一也
  • Journal Title: Journal of General Virology Volume: 94 Issue: 3 Pages: 682-686
  • DOI: 10.1099/vir.0.047860-0
  • Peer Reviewed
• [Journal Article] Identification of three MAPKKKs forming a linear signaling pathway leading to programmed cell death in Nicotiana benthamiana. (2012)
  • Author(s): 橋本 将典
  • Journal Title: BMC Plant Biology Volume: 12 Issue: 1 Pages: 103-103
  • DOI: 10.1186/1471-2229-12-103
  • Peer Reviewed
• [Journal Article] Functional characterization and gene expression profiling of superoxidedismutase from plant pathogenic phytoplasma (2012)
  • Author(s): Miura C. , Sugawara K., Neriya Y., Minato N. , Keima T. , Himeno M., Maejima K., Komatsu K. , Yamaji Y., Oshima K. , Namba S.
  • Journal Title: Gene Volume: 510 Issue: 2 Pages: 107-112
  • DOI: 10.1016/j.gene.2012.09.001
  • Peer Reviewed
• [Journal Article] Infection of capilloviruses requires subgenomic RNAs whose transcription is controlled by promoter-like sequences conserved among flexiviruses (2012)
  • Author(s): Komatsu K., Hirata H., Fukagawa T., Yamaji Y., Okano Y., Ishikawa K., Adachi T., Maejima K., Hashimoto M., Namba S.
  • Journal Title: Virus Research Volume: (印刷中) Issue: 1 Pages: 8-15
  • DOI: 10.1016/j.virusres.2012.02.019
  • Peer Reviewed
• [Journal Article] Identification and characterization of two novel genomic RNA segments of fig mosaic virus, RNA5 and RNA6 (2012)
  • Author(s): Ishikawa K., Maejima K,, Komatsu K., Kitazawa Y., Hashimoto M., Takata D., Yamaji Y., Namba S.
  • Journal Title: Journal of General Virology Volume: 93 Issue: 7 Pages: 1612-1619
  • DOI: 10.1099/vir.0.042663-0
  • Peer Reviewed
• [Presentation] アグロインフィルトレーション法によるpepino mosaic virus接種系の確立 (2016)
  • Author(s): 西田萩子・吉田哲也・遊佐 礼・煉谷裕太朗・薦田 (萩原) 優香・岡野夕香里・橋本将典・山次康幸・難波成任
  • Organizer: 平成２８年度日本植物病理学会大会
  • Place of Presentation: 岡山
  • Year and Date: 2016-03-21
• [Presentation] 本邦産アジサイから検出されたhydrangea ringspot virusの全ゲノム配列 (2016)
  • Author(s): 遊佐 礼・岩渕 望・鯉沼宏章・西田荻子・桂馬拓也・煉谷裕太朗・前島健作・山次康幸・難波成任
  • Organizer: 平成２８年度日本植物病理学会大会
  • Place of Presentation: 岡山
  • Year and Date: 2016-03-21
• [Presentation] lolium latent virusのアグロイノキュレーション用コンストラクトの構築 (2015)
  • Author(s): 西田萩子・吉田哲也・笹野百花・煉谷裕太朗・薦田 (萩原) 優香・岡野夕香里・橋本将典・山次康幸・難波成任
  • Organizer: 平成２７年度日本植物病理学会関東部会
  • Place of Presentation: 宇都宮
  • Year and Date: 2015-09-10
• [Presentation] plantago asiatica mosaic virus のTGBp1 は植物内在性の小分子RNA「tasiRNA」の生成経路を抑制する (2015)
  • Author(s): 二條貴通・岡野夕香里・煉谷裕太 朗・橋本将典・遊佐 礼・桂馬拓 也・大島研郎・小松 健・山次康 幸・難波成任
  • Organizer: 平成２７年度日本植物病理学会大会
  • Place of Presentation: 明治大学（東京）
  • Year and Date: 2015-03-29 – 2015-03-31
• [Presentation] plantago asiatica mosaic virus のTGBp1はtasiRNA 生成経路の2 本鎖RNA 合成を阻害する (2015)
  • Author(s): 桂馬拓也・岡野夕香里・千秋博 子・根津 修・遊佐 礼・二條貴 通・小松 健・山次康幸・難波成任
  • Organizer: 平成２７年度日本植物病理学会大会
  • Place of Presentation: 明治大学（東京）
  • Year and Date: 2015-03-29 – 2015-03-31
• [Presentation] plantago asiatica mosaic virus のTGBp1はRDR6 およびSGS3 と結合してウイルス蓄積量を増加させる (2015)
  • Author(s): 遊佐 礼・ 岡野夕香里・薦田（萩原）優香・桂馬拓也・二條貴通・小松健・山次康幸・難波成任
  • Organizer: 平成２７年度日本植物病理学会大会
  • Place of Presentation: 明治大学（東京）
  • Year and Date: 2015-03-29 – 2015-03-31
• [Presentation] plantago asiatica mosaic virus のTGBp1はホモオリゴマーを形成しSGS3/RDR6 body を凝集させる (2015)
  • Author(s): 岡野夕香里・遊佐 礼・薦田（萩原）優香・桂馬拓也・二條貴通・小松 健・山次康幸・難波成任
  • Organizer: 平成２７年度日本植物病理学会大会
  • Place of Presentation: 明治大学（東京）
  • Year and Date: 2015-03-29 – 2015-03-31
• [Presentation] ダイコンモザイクウイルスの感染による茎頂壊死の誘導因子 (2015)
  • Author(s): 橋本将典・遊佐 礼・友光達哉・桂馬拓也・岡野夕香里・小松 健・山次康幸・難波成任
  • Organizer: 平成２７年度日本植物病理学会大会
  • Place of Presentation: 明治大学（東京）
  • Year and Date: 2015-03-29 – 2015-03-31
• [Presentation] 植物ウイルス感染による細胞死誘導のシグナル伝達経路におけるMAPKKK群の相互関係 (2014)
  • Author(s): 橋本将典・吉田哲也・小松 健・ 岡野夕香里・石川一也・山次康幸・ 難波成任
  • Organizer: 平成２６年度日本植物病理学会大会
  • Place of Presentation: 札幌コンベンションセンター（北海道）
  • Year and Date: 2014-06-02 – 2014-06-04
• [Presentation] 接ぎ木接種法によるJAX1 抵抗性打破potato virus X 変異株の単離 (2014)
  • Author(s): 白石拓也・吉田哲也・北沢優悟・菅 原杏子・根津 修・山次康幸・難波成 任
  • Organizer: 平成２６年度日本植物病理学会大会
  • Place of Presentation: 札幌コンベンションセンター（北海道）
  • Year and Date: 2014-06-02 – 2014-06-04
• [Presentation] JAX1 抵抗性打破を引き起こすpotatovirus X の複製酵素1 アミノ酸変異の解析 (2014)
  • Author(s): 吉田哲也・白石拓也・北沢優悟・菅 原杏子・根津 修・山次康幸・難波成 任
  • Organizer: 平成２６年度日本植物病理学会大会
  • Place of Presentation: 札幌コンベンションセンター（北海道）
  • Year and Date: 2014-06-02 – 2014-06-04
• [Presentation] レクチン抵抗性 : 新規な植物ウイルス耐性システム (2013)
  • Author(s): 山次康幸・前島健作・尾関丈二・小松健・難波成任
  • Organizer: 第11回植物ウイルス病研究会
  • Place of Presentation: 岐阜大学(岐阜県)(招待講演)
  • Year and Date: 2013-03-30
• [Presentation] ウイルスに対する植物免疫システム (2013)
  • Author(s): 山次康幸
  • Organizer: 日本農芸化学会関東支部 2012年度 第2回支部例会
  • Place of Presentation: 茨城大学(茨城県)(招待講演)
  • Year and Date: 2013-02-09
• [Presentation] レクチン抵抗性は真正抵抗性やRNAサイレンシングとは異なるメカニズムにより誘導される (2013)
  • Author(s): 吉田哲也・白石拓也・小松健・山次康幸・難波成任
  • Organizer: 平成25年度日本植物病理学会関東部会
  • Place of Presentation: 法政大学（東京都）
• [Presentation] JAX1-mediated resistance as the key to lectin-triggered immunity (2013)
  • Author(s): Shiraishi, T., Sugawara,K., Yoshida, T., Yamaji, Y.
  • Organizer: EMBO Workshop “Green viruses, from gene to landscape”
  • Place of Presentation: フランス・イエール
• [Presentation] Mechanisms underlying potexvirus-induced systemic necrosis in plants (2013)
  • Author(s): Komatsu, K., Yoshida, T., Yamaji, Y.
  • Organizer: EMBO Workshop “Green viruses, from gene to landscape”
  • Place of Presentation: フランス・イエール
• [Presentation] 植物ウイルスに対する分子耐性機構に関する研究 (2012)
  • Author(s): 山次康幸
  • Organizer: 平成24年度日本農学進歩賞受賞講演会
  • Place of Presentation: 東京大学(東京都)(招待講演)
  • Year and Date: 2012-11-26
• [Presentation] Lectin-Mediated Resistance As a Novel and Universal Innate Immunity Toward Plant Viruses. (2012)
  • Author(s): Yamaji Y, Maejima K, Komatsu K, Shiraishi T, Okano Y, Himeno M., Sugawara K, Neriya Y, Minato N, Miura C, Hashimoto M, Namba S
  • Organizer: XV International Congress of Molecular Plant-Microbe Interactions
  • Place of Presentation: 京都国際会議場(京都府)
  • Year and Date: 2012-08-02