Modification of protein synthesis machinery of silkworms for the production of silk proteins bearing unnatural amino acids
Project/Area Number |
24688008
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Research Category |
Grant-in-Aid for Young Scientists (A)
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Allocation Type | Partial Multi-year Fund |
Research Field |
Applied entomology
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Research Institution | National Institute of Agrobiological Sciences |
Principal Investigator |
TERAMOTO Hidetoshi 独立行政法人農業生物資源研究所, 新機能素材研究開発ユニット, 主任研究員 (60391562)
|
Project Period (FY) |
2012-04-01 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥13,780,000 (Direct Cost: ¥10,600,000、Indirect Cost: ¥3,180,000)
Fiscal Year 2014: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2012: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
|
Keywords | 昆虫 / 生体機能利用 / バイオテクノロジー / 非天然アミノ酸 / other genkou |
Outline of Final Research Achievements |
This study aimed at developing a method to incorporate unnatural amino acids bearing functional groups available for selective chemical reactions (click reactions) into silk proteins in order to provide high-performance silk-based materials to biomedical fields. We have succeeded in incorporating unnatural amino acids bearing azide groups (AzPhe) available for click reactions into silk proteins using genetic modification techniques of silkworms. We verified that azide groups incorporated into silk proteins can be selectively modified with functional molecules using click reactions.
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Report
(5 results)
Research Products
(15 results)