| Budget Amount *help |
¥26,910,000 (Direct Cost: ¥20,700,000、Indirect Cost: ¥6,210,000)
Fiscal Year 2014: ¥10,270,000 (Direct Cost: ¥7,900,000、Indirect Cost: ¥2,370,000)
Fiscal Year 2013: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
Fiscal Year 2012: ¥10,010,000 (Direct Cost: ¥7,700,000、Indirect Cost: ¥2,310,000)
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| Outline of Final Research Achievements |
By the gene cluster analysis method based on amino acid hydroxylase (DO), biosynthetic pathway elucidation of novel peptide molecular species was carried out. A partial peptide biosynthetic pathway was revealed by functional analysis of each constitutive enzyme in non-ribosomal peptide synthesis (NRPS) cluster including a DO gene found in the genome of Burkholderia ambifaria AMMD. Also, novel DOs were found from filamentous fungi to generate hydroxy cyclic amino acids, which often constitute peptides, and were applied to detailed enzymatic characterization analysis. Taking advantage of these results, isotopically labeled hydroxy amino acids were successfully produced by enzyme catalytic processes, and were it possible to trace microbial metabolic intermediates in peptide biosynthesis.
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