Study on post-translational isoprenylation of a tryptophan residue in quorum sensing pheromone
| Project/Area Number |
24688011
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Partial Multi-year Fund |
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| Research Field |
Bioproduction chemistry/Bioorganic chemistry
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| Research Institution | The University of Tokyo (2014-2016)
Chubu University (2012-2013) |
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Principal Investigator |
Okada Masahiro 東京大学, 薬学研究科(研究院), 准教授 (40377792)
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| Project Period (FY) |
2012-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥27,170,000 (Direct Cost: ¥20,900,000、Indirect Cost: ¥6,270,000)
Fiscal Year 2015: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2014: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥14,300,000 (Direct Cost: ¥11,000,000、Indirect Cost: ¥3,300,000)
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| Keywords | クオラムセンシング / 翻訳後修飾 / 枯草菌 / トリプトファン / イソプレニル化 / フェロモン |
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| Outline of Final Research Achievements |
Post-translational isoprenylation of a tryptophan residue was first found in a prokaryotes Bacillus subtilis. In order to evaluate the diversity of post-translational isoprenylation, we focused on the modification enzymes. Based on the information of the essential amino acid residues for the isoprenylation activity, a novel Trp-farnesyltransferase and a novel Trp-farnesylated peptide were found in another phyla of bacteria. Then, X-ray crystal analysis of the Trp-farnesyltransferase was carried out, and then genome mining for proteins sharing the structural features of the enzyme revealed that post-translational isoprenylation of tryptophan was widely distributed in several phyla of bacteria.
In addition, a novel Trp-farnesylated peptide involved in the formation of sticky biofilm was found from B. subtilis subsp. natto. Then, detailed farnesylation analyses of the enzyme revealed that post-translationally isoprenylating enzyme for Trp residue exhibited broad substrate tolerance.
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Report
(6 results)
Research Products
(26 results)
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[Journal Article] Characterization of giant modular PKSs provides insight into genetic mechanism for structural diversification of aminopolyol polyketides.2017
Author(s)
Zhang, L., Hashimoto, T., Qin, B., Hashimoto, J., Kozone, I., Kawahara, T., Okada, M., Awakawa, T., Ito, T., Asakawa, Y., Ueki, M., Takahashi, S., Osada, H., Wakimoto, T., Ikeda, H., Shin-ya, K., Abe, I.
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Journal Title
Ange. Chem. Internal. Edit.
Volume: 56
Pages: 1740-1740
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] An Unusual Chimeric Diterpene Synthase from Emericella variecolor and Its Functional Conversion to a Sesterterpene Synthase by Domain Swapping.2017
Author(s)
Qin, B., Matsuda, Y., Mori, T., Okada, M., Quan, Z., Mitsuhashi, T., Wakimoto, T., Abe, I.
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Journal Title
J. Nat. Prod.,
Volume: 55
Pages: 1658-1661
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Stereospecific prenylation of tryptophan by a cyanobacterial post-translational modification enzyme.2016
Author(s)
Okada, M., Sugita, T., Akita, K., Nakashima, Y., Tian, T., Li, C., Mori, T., Abe, I.
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Journal Title
Org. Biomol. Chem.
Volume: 14
Pages: 9639-9644
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Genome-Based Discovery of an Unprecedented Cyclization Mode in Fungal Sesterterpenoids Biosynthesis.2016
Author(s)
Okada, M., Matsuda, Y., Mitsuhashi, T., Hoshino, S., Mori, T., Nakagawa, K., Quan, Z., Qin, B., Zhang, H., Hayashi, F., Kawaide, H., Abe, I.
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Journal Title
Journal of the American Chemical Society
Volume: 138
Pages: 10011-10018
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Astellifadiene, A Unique Tetracyclic Fungal Sesterterpene: Structure Determination by an NMR-Coupled Crystalline Sponge Method and Elucidation of its Biosynthesis.2016
Author(s)
Matsuda, Y., Mitsuhashi,T., Lee, S., Hoshino, M., Mori, T., Okada, M., Zhang, H., Hayashi, F., Fujita, M., Abe, I.
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Journal Title
Angew. Chem. Int. Ed.
Volume: 55
Pages: 5785-5788
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] S. Hoshino, M. Okada, T. Wakimoto, H. Zhang, F. Hayashi, H. Onaka, and I. Abe. Niizalactams A-C, multicyclic macrolactams isolated from combined-culture of Streptomyces sp. with mycolic acid containing bacterium.2015
Author(s)
S. Hoshino, M. Okada, T. Wakimoto, H. Zhang, F. Hayashi, H. Onaka, I. Abe
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Journal Title
J Nat Prod
Volume: 78
Pages: 3011-3017
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Cration of pure nanodrugs and their anticancer properties2012
Author(s)
H. Kasai, T. Murakami, Y. Ikuta, Y. Koseki, K. Baba, H. Oikawa, H. Nakanishi, M. Okada, M. Shoji, M. Ueda, H. Imahori, M. Hashida
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Journal Title
Biosci. Biotechnol. Biochem.
Volume: 51
Pages: 10315-10318
DOI
Related Report
Peer Reviewed
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