Analysis of intracellular traffic of siRNA
Project/Area Number |
24689005
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Research Category |
Grant-in-Aid for Young Scientists (A)
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Allocation Type | Partial Multi-year Fund |
Research Field |
Biological pharmacy
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Research Institution | National Institute of Health Sciences |
Principal Investigator |
INOUE Takao 国立医薬品食品衛生研究所, 遺伝子医薬部, 室長 (50361605)
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Project Period (FY) |
2012-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥26,520,000 (Direct Cost: ¥20,400,000、Indirect Cost: ¥6,120,000)
Fiscal Year 2014: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
Fiscal Year 2013: ¥8,450,000 (Direct Cost: ¥6,500,000、Indirect Cost: ¥1,950,000)
Fiscal Year 2012: ¥9,490,000 (Direct Cost: ¥7,300,000、Indirect Cost: ¥2,190,000)
|
Keywords | 核酸医薬 / RNAi / siRNA |
Outline of Final Research Achievements |
Oligonucleotide therapeutics, such as siRNA and antisense oligonucleotides, has attracted attention as an innovative medicine for intractable diseases that have no cure. However, it is largely unknown how oligonucleotide therapeutics is incorporated into the cell, and subsequently, transported to the target RNA. In this study, we identified some molecules which are involved in incorporation of oligonucleotides into HEK293 cells.
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Report
(4 results)
Research Products
(23 results)
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[Journal Article] Design and evaluation of locked nucleic acid-based splice-switching oligonucleotides in vitro.2014
Author(s)
Shimo, T., Tachibana, K., Saito, K., Yoshida, T., Tomita, E., Waki, R., Yamamoto, T., Doi, T., Inoue, T., Kawakami, J., Obika, S.
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Journal Title
Nucleic Acids Res.
Volume: 42
Issue: 12
Pages: 8174-8187
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] Evaluation of Off-target Effects of Antisense Oligonucleotides2014
Author(s)
Yoshida, T,, Sasaki, K., Obika, S., Sato, Y., Inoue, T.
Organizer
10th Annual Meeting of the Oligonucleotide Therapeutics Society
Place of Presentation
San Diego
Year and Date
2014-10-13
Related Report
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[Presentation] 核酸医薬品の規制2014
Author(s)
井上貴雄
Organizer
第41回日本毒性学会学術年会
Place of Presentation
兵庫
Year and Date
2014-07-04
Related Report
Invited
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