| Budget Amount *help |
¥26,910,000 (Direct Cost: ¥20,700,000、Indirect Cost: ¥6,210,000)
Fiscal Year 2013: ¥13,520,000 (Direct Cost: ¥10,400,000、Indirect Cost: ¥3,120,000)
Fiscal Year 2012: ¥13,390,000 (Direct Cost: ¥10,300,000、Indirect Cost: ¥3,090,000)
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| Outline of Final Research Achievements |
Ankyrin-G (AnkG) configures the membrane-excitation platform by clustering various ion channels in neurons and cardiomyocytes. AnkG itself localizes to the specific areas on the plasma membrane via the s-palmitoylation of Cys. However, the structural mechanism how AnkG anchors to the membrane is not understood. In this study, we solved the crystal structures of the s-palmitoylation domain of AnkG in reduced and oxidized forms of the Cys, and performed the long-term molecular dynamics simulation of the membrane association. Here we report that the s-palmitoylation facilitated the membrane anchoring of AnkG, defining a stable binding interface to the plasma membrane. AnkG without s-palmitoylation was apparently in contact with the membrane but did not have a unique binding interface. These suggest that AnkG is ready to accept the Cys modification in the juxtamembrane region, and, once it happens, constitutes the rigid structural base of the membrane-excitation platform.
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