Functional analysis of transcription elongation regulatory factor Med26

• Project/Area Number: 24689016
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Partial Multi-year Fund
• Research Field: General medical chemistry
• Research Institution: Hokkaido University
• Principal Investigator: TAKAHASHI Hidehisa 北海道大学, 医学(系)研究科(研究院), 講師 (30423544)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥26,780,000 (Direct Cost: ¥20,600,000、Indirect Cost: ¥6,180,000); Fiscal Year 2014: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000); Fiscal Year 2013: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000); Fiscal Year 2012: ¥10,270,000 (Direct Cost: ¥7,900,000、Indirect Cost: ¥2,370,000)
• Keywords: 生化学 / 転写 / RNAポリメラーゼII / 転写伸長 / メディエーター / MLL / 転写伸長因子 / Pol II

Outline of Final Research Achievements

Regulation of transcription elongation by RNA polymerase II (Pol II) is a key regulatory step in gene transcription. We showed that the human Mediator subunit MED26 plays a role in the recruitment of transcription elongation complexes, SEC and LEC to the different types of genes.
It has been reported that the expression of many genes is strictly controlled and that the dysregulation of the gene expression often causes cancers or leukemias. Since MED26 also regulates the expression of cancer-related genes, it is possible that MED26 is one of the important molecules to regulate carcinogenesis and leukemogenesis.

Research Products

• [Journal Article] MED26 regulates the transcription of snRNA genes through the recruitment of little elongation complex (2015)
  • Author(s): Takahashi, H., Takigawa, I., Watanabe, M., Anwar, D., Shibata, M., Tomomori-Sato, C., Sato, S., Ranjan, A., Seidel, C.W., Tsukiyama, T., Mizushima, W., Hayashi, M., Ohkawa, Y., Conaway, J.W., Conaway, R.C. and Hatakeyama, S
  • Journal Title: Nature Commun. Volume: 5 Issue: 1 Pages: 5941-5941
  • DOI: 10.1038/ncomms6941
  • NAID: 120005621881
  • Peer Reviewed / Open Access
• [Journal Article] The E3 ubiquitin ligase TRIM23 regulates adipocyte differentiation via stabilization of the adipogenic activator PPARγ (2015)
  • Author(s): Watanabe M, Takahashi H, Saeki Y, Ozaki T, Itoh S, Suzuki M, Mizushima W, Tanaka K, Hatakeyama S
  • Journal Title: eLife Volume: in press
  • NAID: 120005617283
  • Peer Reviewed
• [Journal Article] Molecular role of RNF43 in canonical and noncanonical Wnt signaling (2015)
  • Author(s): Tsukiyama T, Fukui A, Terai S, Fujioka Y, Shinada K, Takahashi H, Terry P. Yamaguchi, Ohba Y, and Hatakeyama S
  • Journal Title: Molecular and cellular biology Volume: in press
  • NAID: 120005672365
  • Peer Reviewed
• [Journal Article] The TRIM-FLMN protein TRIM45 directly interacts with RACK1 and negatively regulates PKC-mediated signaling pathway. (2014)
  • Author(s): Sato T., Takahashi H., Hatakeyama S., Iguchi A. and Ariga T
  • Journal Title: Oncogene Volume: In press
  • Peer Reviewed
• [Journal Article] Identification of anti-Sez6l2 antibody in a patient with cerebellar ataxia and retinopathy (2013)
  • Author(s): Yaguchi H, Yabe I, Takahashi H, Okumura F, Takeuchi A, Horiuchi, K, Kan T, Kanda, A, Saito W, Matsumoto M, Nakayama KI, Hatakeyama S and Sasaki H
  • Journal Title: J. Neurol. Volume: 261 Issue: 1 Pages: 224-226
  • DOI: 10.1007/s00415-013-7134-5
  • Peer Reviewed
• [Journal Article] Transcriptional elongation factor elongin A regulates retinoic acid-induced gene expression during neuronal differentiation. (2012)
  • Author(s): Yasukawa T, Bhatt S, Takeuchi T, Kawauchi J, Takahashi H, Tsutsui A, Muraoka T, Inoue M, Tsuda M, Kitajima S, Conaway RC, Conaway JW, Trainor PA, Aso T.
  • Journal Title: Cell Rep Volume: 2(5) Issue: 5 Pages: 1129-36
  • DOI: 10.1016/j.celrep.2012.09.031
  • Peer Reviewed
• [Journal Article] TRIM67 protein negatively regulates Ras activity through degradation of 80K-H and indices neuritogenesis (2012)
  • Author(s): Yaguchi, H., Okumura, F., Takahashi, H., Kano, T., Kameda, H., Uchigashima, M., Tanaka, S., Watanabe, M., Sasaki, H. and Hatakeyama, S.
  • Journal Title: J. Biol. Chem. Volume: 287 Issue: 15 Pages: 12050-12059
  • DOI: 10.1074/jbc.m111.307678
  • Peer Reviewed
• [Presentation] メディエーター複合体による転写伸長制御 (2014)
  • Author(s): 高橋秀尚，瀧川一学，渡部昌，Delnur Anwar，柴田美音，佐藤チエリ，佐藤滋生，Amol Ranjan，Chris W. Seidel，築山忠維，林正康，大川恭行，Joan W. Conaway，Ronald C. Conaway，畠山 鎮次
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜（神奈川県・横浜市）
  • Year and Date: 2014-11-25 – 2014-11-27
  • Invited
• [Presentation] Med26はLittle elongation complexをリクルートすることでsmall nuclear RNA遺伝子の発現を制御する (2014)
  • Author(s): 高橋秀尚，瀧川一学，渡部昌，Anwar Delnur，柴田美音，佐藤チエリ，佐藤滋生，Ranjan Amol，Seidel Chris，築山忠維，林正康，大川恭行，Conaway Joan，Conaway Ronald，畠山鎮次
  • Organizer: 第87回日本生化学会
  • Place of Presentation: 国立京都国際会館（京都府・京都市）
  • Year and Date: 2014-10-15 – 2014-10-18
• [Presentation] Med26はLittle elongation complexをリクルートすることでsmall nuclear RNA遺伝子の発現を制御する (2013)
  • Author(s): 高橋秀尚, 瀧川一学, Delnur Anwar, 柴田美音, 佐藤チエリ, 佐藤滋生, Ranjan Amol, Chris W. Seidel, 築山忠維, 渡部昌, 林正康, 大川恭行, Joan W. Conaway, Ronald C. Conaway, 畠山鎮次
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸ポートピアアイランド（神戸市）
• [Presentation] Human Mediator subunit Med26 regulates the transcription of small nuclear RNA genes through the recruitment of little elongation complex. (2013)
  • Author(s): Hidehisa Takahashi, Chieri Tomomori-Sato, Shigeo Sato, Amol Ranjan, Masayasu Hayashi, Yasuyuki Ohkawa, Joan W. Conaway, Ronald C. Conaway, Shigetsugu Hatakeyama
  • Organizer: Mechanism of eukaryotic transcription, Cold spring harbor meeting
  • Place of Presentation: Cold spring harbor laboratory (USA)
• [Presentation] Med26はLittle elongation complexをリクルートすることでsmall nuclear RNA遺伝子の発現を制御する (2012)
  • Author(s): 高橋秀尚, 佐藤智信, Conaway, J.W., Conaway, R.C., 畠山鎮次
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場、マリンメッセ福岡(福岡)
• [Presentation] Human Mediator subunit Med26 plays a role in recruitment of "Little elongation complex" to small nuclear RNA genes (2012)
  • Author(s): Hidehisa Takahashi, Joan W. Conaway, Ronald C. Conaway, Shigetsugu Hatakeyama
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場、マリンメッセ福岡(福岡)(ポスター)
• [Presentation] Med26はLittle elongation complexをリクルートすることでsmall nuclear RNA遺伝子の発現を制御する (2012)
  • Author(s): 高橋秀尚, 佐藤智信, Conaway, J.W., Conaway, R.C., 畠山鎮次
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡国際会議場、マリンメッセ福岡(福岡)
• [Presentation] TRIM29によるTRRAP/Tip60複合体の機能制御とがん化との関わり (2012)
  • Author(s): 桝田安志、高橋秀尚、畠山鎮次
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡国際会議場、マリンメッセ福岡(福岡)
• [Remarks] 新規転写伸長因子Med26の機能解析
  • URL: http://www.hucc.hokudai.ac.jp/~d20505/takahashi/
• [Remarks] 新規転写伸長制御因子Med26の機能解析
  • URL: http://www.hucc.hokudai.ac.jp/~d20505/takahashi/index.html
• [Remarks]
  • URL: http://www.hucc.hokudai.ac.jp/~d20505/takahashi/index.html