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Coordinated epigenetic regulation of cell type-specific genes and chronic disease development in cardiomyocytes.

Research Project

Project/Area Number 24689037
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypePartial Multi-year Fund
Research Field Circulatory organs internal medicine
Research InstitutionKeio University

Principal Investigator

ODA Mayumi  慶應義塾大学, 医学部, 助教 (80567511)

Research Collaborator SAKOTA Miki  慶應義塾大学, 医学部, 研究員
Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥11,960,000 (Direct Cost: ¥9,200,000、Indirect Cost: ¥2,760,000)
Fiscal Year 2013: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2012: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
Keywordsエピゲノム / DNAメチル化 / 細胞特異的発現遺伝子 / 成熟細胞 / DNA脱メチル化機構 / 病態発生 / エピジェネティック制御 / gene body領域 / 心不全 / 細胞特異的遺伝子 / 疾患モデル動物 / 次世代シークエンサー / トランスクリプトーム / 細胞種特異的発現調節 / ゲノム
Research Abstract

In this study, I made several cell type-specific DNA methylation epigenome data of isolated cardiomyocyte populations from several developmental stages and pushed forward understanding of the epigenome in matured cells. I compared the cardiomyocyte epigenome with those of embryonic stem cells and liver, and also with the heart failure cardiac muscle cells. As the result, small subpopulation of genes with remarkable DNA methylation changes in their gene body regions were included in the cell type-specific gene trait. These gene body regions accumulated the transcription machinery and histone acetylation enzyme in the matured cells, and transiently accumulate the derivatives of methyl cytosine demethylation, which implicates the active demethylation process. Our study showed the involvement of the active DNA demethylation process in gene body regions and indicates the possibility that the gene body DNA hypomethylationmay stabilize the cell type-specific gene transcription.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Annual Research Report
  • Research Products

    (18 results)

All 2014 2013 2012 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (10 results) Remarks (6 results)

  • [Journal Article] Genetic Analysis of Essential Cardiac Transcription Factors in 256 Patients With Non-Syndromic Congenital Heart Defects2012

    • Author(s)
      Kodo K, Nishizawa T, Furutani M, Arai S, Ishihara K, Oda M, Makino S, Fukuda K, Takahashi T, Matsuoka R, Nakanishi T, Yamagishi H
    • Journal Title

      Circulation Journal

      Volume: 76 Issue: 7 Pages: 1703-1711

    • DOI

      10.1253/circj.CJ-11-1389

    • NAID

      10030503588

    • ISSN
      1346-9843, 1347-4820
    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Genetic analysis of essential cardiac transcription factors in 256 patientswith non-syndromic congenital heart defects.2012

    • Author(s)
      Kodo K, Nishizawa T, Furutani M, Arai S, Ishihara K, Oda M, Makino S, Fukuda K, Takahashi T, Matsuoka R, Nakanishi T, Yamagishi H.
    • Journal Title

      Circulation Journal

      Volume: 76 Pages: 1703-7011

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Presentation] 成熟細胞の細胞特異的遺伝子gene body領域における能動的DNA脱メチル化分子機構の関与2014

    • Author(s)
      小田真由美
    • Organizer
      第8回エピジェネティクス研究会年会
    • Place of Presentation
      伊藤国際学術研究センター(東京都)(ポスター発表)
    • Related Report
      2013 Final Research Report
  • [Presentation] DNAメチル化エピゲノム解析による哺乳類gene body DNAメチル化の成り立ちと転写の関係2013

    • Author(s)
      小田真由美
    • Organizer
      NGS現場の会第三回研究会
    • Place of Presentation
      神戸国際会議腸(兵庫県)(ポスター発表)
    • Related Report
      2013 Final Research Report
  • [Presentation] DNAメチル化エピゲノムによるアクセシビリティ調節と細胞特異的遺伝子発現の効率化2013

    • Author(s)
      小田真由美
    • Organizer
      第7回エピジェネティクス研究会年会
    • Place of Presentation
      奈良県新公会堂(奈良県)(ポスター発表)
    • Related Report
      2013 Final Research Report
  • [Presentation] DNA メチル化エピゲノムによるアクセシビリティ調節と細胞特異的遺伝子発現の効率化2013

    • Author(s)
      小田真由美
    • Organizer
      第7回日本エピジェネティクス研究会年会
    • Place of Presentation
      奈良県新公会堂
    • Related Report
      2013 Annual Research Report
  • [Presentation] DNAメチル化エピゲノム解析による哺乳類gene body DNAメチル化の成り立ちと転写の関係2013

    • Author(s)
      小田真由美
    • Organizer
      NGS現場の会第三回年会
    • Place of Presentation
      神戸国際会議場
    • Related Report
      2013 Annual Research Report
  • [Presentation] 細胞特異的な遺伝子発現とDNAメチル化エピゲノム2013

    • Author(s)
      小田真由美
    • Organizer
      五拠点幹細胞シンポジウム
    • Place of Presentation
      有馬温泉(兵庫)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Gene body DNA hypomethylation is associated with enhancement of gene expression via Pol II entrapment in their gene body regions.2013

    • Author(s)
      Mayumi Oda
    • Organizer
      CiRA International Symposium
    • Place of Presentation
      京都大学(京都)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Cell Type-specific Gene body DNA Hypomethylation Contributes to Transcriptional Efficiency in Cardiomyocyte Maturation2012

    • Author(s)
      Mayumi Oda
    • Organizer
      第6回エピジェネティクス研究会年会
    • Place of Presentation
      一橋学術総合センター(東京都)(ポスター発表)
    • Related Report
      2013 Final Research Report
  • [Presentation] Escape of Gene Body DNA Methylation in Cardiac Genes is Cooperative With the Cell Type-specific Expression Patterns2012

    • Author(s)
      Mayumi Oda
    • Organizer
      Weinstein meeting 2012
    • Place of Presentation
      Sheraton Chicago Hotel and Towers(シカゴ・イリノイ州・米国)(口頭発表)
    • Related Report
      2013 Final Research Report
  • [Presentation] 心筋細胞特異的遺伝子のDNAメチル化状態はgene body領域における転写とエピジェネティック状態の関係に影響を及ぼす2012

    • Author(s)
      小田真由美
    • Organizer
      第35回 分子生物学会年会
    • Place of Presentation
      福岡国際会議場・マリンメッセ福岡(福岡)
    • Related Report
      2012 Annual Research Report
  • [Remarks] 坂口講座テニュアトラック・プログラム

    • URL

      http://www.careerpath-prj.keio.ac.jp/sakaguchi/scholar/m_oda/

    • Related Report
      2013 Final Research Report
  • [Remarks] 義塾大学医学部・システム医学講座

    • URL

      http://systemsmedicine.jp

    • Related Report
      2013 Final Research Report
  • [Remarks] 慶應義塾坂口光洋記念講座 坂口講座テニュアトラック・プログラム

    • URL

      http://www.careerpath-prj.keio.ac.jp/sakaguchi/scholar/m_oda/

    • Related Report
      2013 Annual Research Report
  • [Remarks] 小田真由美 - 研究者 - researchmap

    • URL

      http://researchmap.jp/odamame/

    • Related Report
      2013 Annual Research Report
  • [Remarks] 研究内容、研究成果に関するwebページ Research map:

    • URL

      http://researchmap.jp/odamame/

    • Related Report
      2012 Annual Research Report
  • [Remarks] 坂口光洋記念講座ホームページ : 小田真由美

    • URL

      http://www.cereerpath-prj.keio.ac.jp/sakaguchi/scholar/m_oda/

    • Related Report
      2012 Annual Research Report

URL: 

Published: 2012-04-24   Modified: 2019-07-29  

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