Investigating the role of sugar chain in microenvironment of skin cancer

• Project/Area Number: 24689045
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Partial Multi-year Fund
• Research Field: Dermatology
• Research Institution: Osaka University
• Principal Investigator: TERAO mika 大阪大学, 医学(系)研究科(研究院), 特任研究員 (40570669)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000); Fiscal Year 2014: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2013: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2012: ¥9,100,000 (Direct Cost: ¥7,000,000、Indirect Cost: ¥2,100,000)
• Keywords: 糖転移酵素 / GnT-V / M1マクロファージ / M2マクロファージ / 皮膚がん / 悪性黒色腫 / 皮膚硬化 / 強皮症 / 線維芽細胞 / PI3K-Akt

Outline of Final Research Achievements

N-Acetylglucosaminyltransferase-V (GnT-V) expression is elevated during malignant transformation in various types of cancer. To characterize the biological function of GnT-V in macrophages,we analyzed the role of GnT-V using GnT-V knockout (KO) mice. In bone marrow-derived macrophages (BMDMs), IL-4-induced expressions of M2 macrophage markers were reduced in KO mice. In addition, KO mice were resistant to BLM-induced skin sclerosis with reduced number of CD163+ M2 macrophages. These results suggest the importance of GnT-V in differentiation of M2 macrophages in the skin.

Research Products

• [Journal Article] T Helper 2 Polarization in Senile Erythroderma with Elevated Levels of TARC and IgE. (2015)
  • Author(s): Nakano-Tahara M, Terao M, Nishioka M, Kitaba S, Murota H, Katayama I
  • Journal Title: Dermatology Volume: in press
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] A vitamin D analog inhibits Th2 cytokine- and TGFβ -induced periostin production in fibroblasts: a potential role for vitamin D in skin sclerosis (2015)
  • Author(s): Terao M, Yang L, Matsumura S, Yutani M, Murota H, Katayama I
  • Journal Title: Dermato-Endocrinology Volume: in press
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] 11β-hydroxysteroid dehydrogenase 1 specific inhibitor increased dermal collagen content and promotes fibroblast proliferation. (2014)
  • Author(s): Terao M, Tani M, Itoi S, Yoshimura T, Hamasaki T, Murota H, Katayama I.
  • Journal Title: PLoS One. Volume: Mar 25;9(3) Issue: 3 Pages: e93051-e93051
  • DOI: 10.1371/journal.pone.0093051
  • Peer Reviewed / Open Access
• [Journal Article] Immunohistochemical Analysis of Interleukin-17 Producing T Helper Cells and Regulatory T Cells Infiltration in Annular Erythema Associated with Sjögren's Syndrome (2014)
  • Author(s): Itoi S, Tanemura A, Tani M, Kitaba S, Terao M, Murota H, Oiso N, Katayama I
  • Journal Title: Ann Dermatol Volume: 26(2) Issue: 2 Pages: 203-8
  • DOI: 10.5021/ad.2014.26.2.203
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] IFN-γ or IFN-α ameliorates chronic proliferative dermatitis by inducing expression of linear ubiquitin chain assembly complex. (2014)
  • Author(s): Tamiya H, Terao M, Takiuchi T, Nakahara M, Sasaki Y, Katayama I, Yoshikawa H, and Iwai K.
  • Journal Title: J. Immunol. Volume: 192 Issue: 8 Pages: 3793-3804
  • DOI: 10.4049/jimmunol.1302308
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Topical cholesterol treatment ameliorates hapten-evoked cutaneous persensitivity by sustaining expression of 11β-HSD1 in epidermis. (2014)
  • Author(s): Murota H, Itoi S, Terao M, Matsui S, Kawai H, Satou Y, Suda K, Katayama I
  • Journal Title: Experimental Dermatology Volume: Jan;23(1) Issue: 1 Pages: 68-70
  • DOI: 10.1111/exd.12284
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] IFN-γ or IFN-α Ameliorates Chronic Proliferative Dermatitis by Inducing (2014)
  • Author(s): Tamiya H, Terao M, Takiuchi T, Nakahara M, Sasaki Y, Katayama I, Yoshikawa H,
  • Journal Title: Journal of Immunology Volume: 2014 Mar 14
  • Peer Reviewed
• [Journal Article] Expression profiles of cortisol-inactivating enzyme, 11β-hydroxysteroid dehydrogenase-2, in human epidermal tumors and its role in keratinocyte proliferation. (2013)
  • Author(s): Terao M, Itoi S, Murota H, Katayama I.
  • Journal Title: Exp Dermatol. Volume: Feb;22(2) Issue: 2 Pages: 98-101
  • DOI: 10.1111/exd.12075
  • Peer Reviewed
• [Journal Article] 11β-Hydroxysteroid dehydrogenase 1 contributes to the pro-inflammatory response of keratinocytes. (2013)
  • Author(s): Itoi S, Terao M, Murota H, Katayama I.
  • Journal Title: Biochem Biophys Res Commun. Volume: Oct 18;440(2) Issue: 2 Pages: 265-70
  • DOI: 10.1016/j.bbrc.2013.09.065
  • Peer Reviewed
• [Journal Article] Barrier abnormality due to ceramide deficiency leads to psoriasiform inflammation in a mouse model. (2013)
  • Author(s): Nakajima K, Terao M, Takaishi M, Kataoka S, Goto-Inoue N, Setou M, Horie K, Sakamoto F, Ito M, Azukizawa H, Kitaba S, Murota H, Itami S, Katayama I, Takeda J, Sano S.
  • Journal Title: J Invest Dermatol. Volume: 133 Issue: 11 Pages: 2555-2565
  • DOI: 10.1038/jid.2013.199
  • Peer Reviewed
• [Journal Article] Upregulation of N-acetylglucosaminyltransferase-V by heparin-binding EGF-like growth factor induces keratinocyte proliferation and epidermal hyperplasia. (2012)
  • Author(s): Kimura A, Terao M, Kato A, Hanafusa T, Murota H, Katayama I, Miyoshi E.
  • Journal Title: Exp Dermatol. Volume: 21(7) Issue: 7 Pages: 515-9
  • DOI: 10.1111/j.1600-0625.2012.01515.x
  • Peer Reviewed
• [Journal Article] Physiological roles of Nacetylglucosaminyl transferase V(GnT-V) in mice (2012)
  • Author(s): Miyoshi E, Terao M, Kamada Y
  • Journal Title: BMB Reports Volume: 45(10) Pages: 554-559
  • Peer Reviewed
• [Journal Article] Vitiligo exacerbated after herpes zoster (2012)
  • Author(s): Terao M, Tanemura A, Katayama I
  • Journal Title: Journal of Dermatology Volume: 39(11) Issue: 11 Pages: 938-939
  • DOI: 10.1111/j.1346-8138.2012.01514.x
  • Peer Reviewed
• [Journal Article] Periostin Facilitates Skin Sclerosis via PI3K/Akt Dependent Mechanism in a Mouse Model of Scleroderma (2012)
  • Author(s): Yang L, Serada S, Fujimoto M, Terao M, Kotobuki Y, Kitaba S, Matsui S, Kudo A, Naka T, Murota H, Katayama I
  • Journal Title: PLoS One Volume: 7(7) Issue: 7 Pages: e41994-e41994
  • DOI: 10.1371/journal.pone.0041994
  • Peer Reviewed
• [Journal Article] Artemin causes hypersensitivity to warm sensation, mimicking warmth-provoked pruritus in atopic dermatitis. (2012)
  • Author(s): Murota H, Izumi M, Abd El-Latif MI, Nishioka M, Terao M, Tani M, Matsui S, Sano S, Katayama I.
  • Journal Title: Journal of Allergy and Clinical Immunology Volume: 130 Issue: 3 Pages: 671-682
  • DOI: 10.1016/j.jaci.2012.05.027
  • Peer Reviewed
• [Presentation] Local cortisol activation by 11b-hydroxysteroid dehydrogenase 1 in keratinocytes down regulates skin inflammation (2014)
  • Author(s): Mika Terao, Saori Itoi, Shun Kitaba, Hiroyuki Murota, Ichiro Katayama
  • Organizer: The 39th Annual Meeting of the Japanese Society for Investigative Dermatology
  • Place of Presentation: Osaka, Japan
  • Year and Date: 2014-12-12 – 2014-12-14
• [Presentation] Oligosaccharide modification by GnT-V augments oxazolone-induced atopic dermatitis-like symptoms (2014)
  • Author(s): Mizuki Yutani, Mika Terao, Arisa Kato, Hiroyuki Murota, Eiji Miyoshi, Ichiro Katayama
  • Organizer: The 39th Annual Meeting of the Japanese Society for Investigative Dermatology
  • Place of Presentation: Osaka, Japan
  • Year and Date: 2014-12-12 – 2014-12-14
• [Presentation] The role of 11β-hydroxysteroid dehydrogenase 1 in UVB-induced skin inflammation (2014)
  • Author(s): Saori Itoi, Mika Terao, Hiroyuki Murota, Ichiro Katayama
  • Organizer: The 39th Annual Meeting of the Japanese Society for Investigative Dermatology
  • Place of Presentation: Osaka, Japan
  • Year and Date: 2014-12-12 – 2014-12-14
• [Presentation] Th2 cytokines enhance TrkA expression and upregulate proliferation and downregulate differentiation of keratinocytes (2014)
  • Author(s): Sayaka Matsumura, Mika Terao, Hiroyuki Murota, Ichiro Katayama
  • Organizer: The 39th Annual Meeting of the Japanese Society for Investigative Dermatology
  • Place of Presentation: Osaka, Japan
  • Year and Date: 2014-12-12 – 2014-12-14
• [Presentation] 皮膚炎症における細胞内コルチゾール再活性化酵素（11β―HSD1）の動態解析 (2014)
  • Author(s): 越智沙織 寺尾美香 室田浩之 片山一朗
  • Organizer: 北海道地方会398回例会
  • Place of Presentation: 旭川
  • Year and Date: 2014-06-07
• [Presentation] Activation of local cortisol by 11β-HSD1 in keratinocytes is important in supressing local inflammation (2014)
  • Author(s): Mika Terao, Saori Itoi, Hiroyuki Murota, Ichiro Katayama
  • Organizer: International Symposium on Atopic Dermatitis/8th Georg Rajka Symposium
  • Place of Presentation: Nottingham, UK
  • Year and Date: 2014-05-21 – 2014-05-23
• [Presentation] 皮膚炎症における細胞内コルチゾール再活性化酵素（11β―HSD1）の検討 (2014)
  • Author(s): 越智沙織 寺尾美香 室田浩之 片山一朗
  • Organizer: 第26回日本アレルギー学会春季臨床大会
  • Place of Presentation: 京都
  • Year and Date: 2014-05-09 – 2014-05-11
• [Presentation] アトピー性皮膚炎における角層内水分量と発疹型との相関について (2014)
  • Author(s): 高橋 彩 木村明寛 松井佐起 木島晶子 寺尾美香 室田浩之 片山一朗
  • Organizer: 第26回日本アレルギー学会春季臨床大会
  • Place of Presentation: 京都
  • Year and Date: 2014-05-09 – 2014-05-11
• [Presentation] Oligosaccharide modification by N-acetylglucosaminyltransferase-V promotes skin sclerosis by inducing macrophages to shift toward M2. (2013)
  • Author(s): Mika Terao, Arisa Kato, Mizuki Yutani, Hiroyuki Murota, Eiji Miyoshi, Ichiro Katayama
  • Organizer: 2013 International Investigative Dermatology Meeting
  • Place of Presentation: Edinburgh, Scotland
• [Presentation] Expression profiles of cortisol inactivating enzyme, 11β-hydroxysteroid dehydrogenase 1 and 2 in human epidermal tumors and its role in keratinocyte proliferation. (2013)
  • Author(s): Mika Terao, Hiroyuki Murota, Ichiro Katayama
  • Organizer: 1st Dermatoendocrinology Meeting
  • Place of Presentation: Edinburgh, Scotland
• [Presentation] Oligosaccharide modification by N-acetylglucosaminyltransferase-V promotes skin sclerosis by inducing macrophages to shift toward M2 (2013)
  • Author(s): 油谷美寿季、寺尾美香、加藤亜里沙、室田浩之、片山一朗、三善英知
  • Organizer: 第８６回日本生化学学会
  • Place of Presentation: 横浜
• [Presentation] Topical Vitamin D3 Therapy for Steroid Resistant Prurigo: Anti-inflammatory Action or Neuroprotective Effect (2013)
  • Author(s): Ichiro Katayama, Mika Terao, Mayuko Nakano, SJ Bae, Hiroyuki Murota
  • Organizer: 7th World Congress on ITCH
  • Place of Presentation: Boston, USA
• [Presentation] Topical cholesterol application ameliorates hapten-evoked cutaneous hypersensitivity by sustaining expression of 11βHSD-1 in epidermis. (2013)
  • Author(s): H Murota, M Terao, S Matsui, H Kawai, Y Satou, K Suda, I Katayama
  • Organizer: 2013 International Investigative Dermatology Meeting
  • Place of Presentation: Edinburgh, Scotland
• [Presentation] Upregulation of periostin by histamine via ERK1/2 pathway is essential for histamine-induced collagen production in dermal fibroblasts (2013)
  • Author(s): Linli Yang, Hiroyuki Murota, Saki Matsui, Mika Terao, Ichiro Katayama
  • Organizer: 2013 International Investigative Dermatology Meeting
  • Place of Presentation: Edinburgh, Scotland
• [Presentation] Oligosaccharide modification by GnT-V promotes skin sclerosis bymodulating the recruitment/activation of macrophage. (2012)
  • Author(s): Arisa Kato, Mika Terao. Mizuki Yutani, Hiroyuki Murota, Eiji Miyoshi, Ichiro Katayama
  • Organizer: 37th Japanese Society for Investigative Dermatology
  • Place of Presentation: Okinawa, Japan
  • Year and Date: 2012-12-09
• [Presentation] Local Cortisol activation by 116-HSD1 progresses skin atrophy through reducing the proliferation of fibroblasts in adult mouse skin. (2012)
  • Author(s): Mika Terao. Saori Itoi, Mamori Tani, Hiroyuki Murota, Ichiro Katayama
  • Organizer: 42th European Society for Investigative Dermatology
  • Place of Presentation: Venice, Italy
  • Year and Date: 2012-09-22
• [Presentation] Soluble GnT-V shift toward M2 macrophage in transgenic mouse skin (2012)
  • Author(s): Arisa Kato, Mika Terao. Mizuki Yutani, Hiroyuki Murota, Eiji Miyoshi, Ichiro Katayama
  • Organizer: 42th European Society for Investigative Dermatology,
  • Place of Presentation: Venice, Italy
  • Year and Date: 2012-09-21
• [Book] 幼小児によくみられる皮膚疾患アトラス 鑑別と治療のポイント １３（５）色素性乾皮症 (2015)
  • Author(s): 寺尾美香（編：横関博雄，片山一朗）
  • Total Pages: 2
  • Publisher: 医薬ジャーナル社
• [Book] 皮膚のステロイド代謝と皮膚老化～11β―HSD1を中心に～ (2014)
  • Author(s): 寺尾美香（編：片山一朗）
  • Total Pages: 10
  • Publisher: 医薬ジャーナル社