Generation of vascularized and functional tissues from human iPS cell

• Project/Area Number: 24689052
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Partial Multi-year Fund
• Research Field: General surgery
• Research Institution: Yokohama City University
• Principal Investigator: TAKEBE TAKANORI 横浜市立大学, 医学部, 准教授 (20612625)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥25,610,000 (Direct Cost: ¥19,700,000、Indirect Cost: ¥5,910,000); Fiscal Year 2014: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000); Fiscal Year 2013: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000); Fiscal Year 2012: ¥11,830,000 (Direct Cost: ¥9,100,000、Indirect Cost: ¥2,730,000)
• Keywords: 再生医学 / iPS細胞 / 肝臓 / 膵臓 / 前駆細胞 / 三次元培養

Outline of Final Research Achievements

Critical shortage of donor organs for treating end-stage organ failure highlights the urgent need for generating organs from induced pluripotent stem cells (hiPSCs). Despite many reports describing functional cell differentiation, no studies have succeeded in generating a three-dimensional vascularised organ such as liver. In this program, we showed the generation of vascularised and functional human liver from hiPSCs by transplantation of liver buds created in vitro (hiPSC-LBs). Mesenteric transplantation of hiPSC-LBs rescued the drug-induced lethal liver failure model. Furthermore, similar principle was extended to generate vascularized pancreatic islet towards therapy. Although efforts must ensue to translate these techniques to patients, our proof-of-concept, i.e. organ bud transplantation, provides a promising new approach towards regenerative medicine.

Research Products

• [Journal Article] Transient vascularization of transplanted human adult–derived progenitors promotes self-organizing cartilage. (2014)
  • Author(s): Takebe T *, Kobayashi S, Suzuki H, Mizuno M, Chang YM, Yoshizawa E, Kimura M, Hori A, Asano J, Maegawa J, Taniguchi H
  • Journal Title: Journal of Clinical Investigation Volume: 124 Issue: 10 Pages: 4325-4334
  • DOI: 10.1172/jci76443
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Generation of a vascularized and functional human liver from an iPSC-derived organ bud transplant. (2014)
  • Author(s): Takanori Takebe*, Ran-Ran Zhang, Hiroyuki Koike, Masaki Kimura, Emi Yoshizawa, Masahiro Enomura, Keisuke Sekine, Hideki Taniguchi*(*: Correspondence)
  • Journal Title: Nature Protocols Volume: 9(2) Issue: 2 Pages: 396-409
  • DOI: 10.1038/nprot.2014.020
  • Peer Reviewed
• [Journal Article] Effect of cryopreservation and cell passage number on cell preparations destined for autologous chondrocyte transplantation. (2014)
  • Author(s): Nam BM, Kim BY, Jo YH, Lee S, Nemeno JG, Yang W, Lee KM, Kim H, Jang IJ, Takebe T, Lee JI
  • Journal Title: Transplant Proc Volume: 46 Issue: 4 Pages: 1145-1149
  • DOI: 10.1016/j.transproceed.2013.11.117
  • Peer Reviewed
• [Journal Article] Polycomb group protein Ezh2 regulates hepatic progenitor cell proliferation and differentiation in murine embryonic liver. (2014)
  • Author(s): Koike H, Ouchi R, Ueno Y, Nakata S, Obana Y, Sekine K, Zheng YW, Takebe T, Isono K, Koseki H, Taniguchi H.
  • Journal Title: PLoS One. Volume: 9 Issue: 8 Pages: 0-2
  • DOI: 10.1371/journal.pone.0104776
  • Peer Reviewed / Open Access
• [Journal Article] Effect of Preservation Conditions on Cartilage Tissue for Cell Transplantation. (2014)
  • Author(s): Kim BY, Nam BM, Lee KM, Jo YH, Nemeno JG, Yang W, Lee S, Kim H, Jang IJ, Takebe T, Lee JI
  • Journal Title: Transplant Proc Volume: 46 Issue: 4 Pages: 1139-1144
  • DOI: 10.1016/j.transproceed.2013.11.125
  • Peer Reviewed
• [Journal Article] Artificial Islets From Hybrid Spheroids of Three Pancreatic Cell Lines. (2014)
  • Author(s): Jo YH, Jang IJ, Nemeno JG, Lee S, Kim BY, Nam BM, Yang W, Lee KM, Kim H, Takebe T, Kim YS, Lee JI
  • Journal Title: Transplant Proc Volume: 46 Issue: 4 Pages: 1156-1160
  • DOI: 10.1016/j.transproceed.2013.11.074
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Effects of Natural Cartilaginous Extracellular Matrix on Chondrogenic Potential for Cartilage Cell Transplantation. (2014)
  • Author(s): Yang W, Lee S, Jo YH, Lee KM, Nemeno JG, Nam BM, Kim BY, Jang IJ, Kim HN, Takebe T, Lee JI
  • Journal Title: Transplant Proc Volume: 46 Issue: 4 Pages: 1247-1250
  • DOI: 10.1016/j.transproceed.2013.11.082
  • Peer Reviewed
• [Journal Article] The evidence of sophisticatedly heterogeneous population of human umbilical vein endothelial cells (2014)
  • Author(s): Zheng YW, Nie YZ, Tsuchida T, Zhang RR, Aoki K, Sekine K, Ogawa M, Takebe T, Ueno Y, Sakakibara H, Hirahara F, Taniguchi H
  • Journal Title: Transplant Proc Volume: 46 Issue: 4 Pages: 1251-3
  • DOI: 10.1016/j.transproceed.2013.11.077
  • Peer Reviewed / Open Access
• [Journal Article] Fluorescent Labeling and Visualization of Human Induced Pluripotent Stem Cells With the Use of Transcription Activator–like Effector Nucleases. (2014)
  • Author(s): Sekine K, Takebe T, Taniguchi H
  • Journal Title: Transplant Proc Volume: 46 Pages: 1205-1207
  • Peer Reviewed
• [Journal Article] The CD133+CD44+ Precancerous Subpopulation of Oval Cells is a Therapeutic Target for Hepatocellular Carcinoma (2014)
  • Author(s): Zheng YW, Tsuchida T, Shimao T, Li B, Takebe T, Zhang RR, Sakurai Y, Ueno Y, Sekine K, Ishibashi N, Imajima M, Tanaka T, and Taniguchi H
  • Journal Title: Stem Cells Dev Volume: (In press) Issue: 18 Pages: 2237-2249
  • DOI: 10.1089/scd.2013.0577
  • Peer Reviewed / Open Access
• [Journal Article] Ring1B Promotes Hepatic Stem/Progenitor Cell Expansion via Simultaneous Suppression of Cdkn1a and Cdkn2a. (2014)
  • Author(s): Koike H, Ueno Y, Naito T, Shiina T, Nakata S, Ouchi R, Obana Y, Sekine K, Zheng YW, Takebe T, Isono K, Koseki H, Taniguchi H
  • Journal Title: Hepatology Volume: 印刷中 Issue: 1 Pages: 323-333
  • DOI: 10.1002/hep.27046
  • Peer Reviewed / Open Access
• [Journal Article] The development of humanized liver with Rag1 knockout rats. (2014)
  • Author(s): Tsuchida T, Zheng YW, Zhang RR, Takebe T, Ueno Y, Sekine K, Taniguchi H
  • Journal Title: Transplant Proc Volume: 46 Issue: 4 Pages: 1191-3
  • DOI: 10.1016/j.transproceed.2013.12.026
  • Peer Reviewed / Open Access
• [Journal Article] Tropism of cancer stem cells to a specific distant organ. (2014)
  • Author(s): Okuda R, Sekine K, Hisamatsu D, Ueno Y, Takebe T, Zheng YW, Taniguchi H
  • Journal Title: In Vivo Volume: 28 Pages: 361-365
  • Peer Reviewed
• [Journal Article] Elastic cartilage reconstruction by transplantation of cultured hyaline cartilage-derived chondrocytes. (2014)
  • Author(s): Mitsuru Mizuno, Takanori Takebe, Shinji Kobayashi, Shintarou Kimura, Mamiko Masutani, Soojung Lee, Yong Hwa Jo, Jeong Ik Lee, Hideki Taniguchi
  • Journal Title: Transplant Proc, Volume: 46 Issue: 4 Pages: 1217-1221
  • DOI: 10.1016/j.transproceed.2013.12.006
  • Peer Reviewed
• [Journal Article] Identification of Proliferating Human Hepatic Cells from Human Induced Pluripotent Stem Cells (2014)
  • Author(s): Zhang RR, Takebe T, Sekine K, Koike H, Zheng YW, Taniguchi H
  • Journal Title: Transplant Proc Volume: 46(4) Issue: 4 Pages: 1201-4
  • DOI: 10.1016/j.transproceed.2013.12.021
  • Peer Reviewed
• [Journal Article] Engineering of human hepatic tissue with functional vascular networks (2014)
  • Author(s): Takebe T, Koike N, Sekine K, Fujiwara R, Amiya T, Zheng YW and Taniguchi H
  • Journal Title: Organogenesis Volume: 10(2)(Epub ahead of print) Issue: 2 Pages: 260-267
  • DOI: 10.4161/org.27590
  • Peer Reviewed
• [Journal Article] High-resolution intravital imaging for monitoring the transplanted islets in mice. (2014)
  • Author(s): Takahashi Y, Takebe T, Enomura M, Koike N, Lee S, Nemeno JG, Sekine K, Lee JI, Taniguchi H.
  • Journal Title: Transplant Proc. Volume: 46 Issue: 4 Pages: 1166-1168
  • DOI: 10.1016/j.transproceed.2013.11.089
  • Peer Reviewed
• [Journal Article] Self-Formation of Vascularized Hepatic Tissue from Human Adult Hepatocyte. (2014)
  • Author(s): Enomoto Y, Enomura M, Takebe T*, Mitsuhashi Y, Kimura M, Yoshizawa E, Taniguchi H*
  • Journal Title: Transplant Proc Volume: 46 Issue: 4 Pages: 1243-1246
  • DOI: 10.1016/j.transproceed.2013.11.086
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Reconstruction of Joint Hyaline Cartilage by Autologous Progenitor Cell s Derived from Ear Elastic Cartilage (2014)
  • Author(s): M. Mizuno, S. Kobayashi, T. Takebe, H. Kan, Y. Yabuki, T. Matsuzaki, H. Y. Yoshikawa, S. Nakabayashi, L. J. Ik, J. Maegawa, H. Taniguchi
  • Journal Title: Stem Cells Volume: 32 Issue: 3 Pages: 816-821
  • DOI: 10.1002/stem.1529
  • Peer Reviewed / Open Access
• [Journal Article] Vascularized and functional human liver from an iPSC-derived organ bud transplant. (2013)
  • Author(s): Takanori Takebe*, Keisuke Sekine, Masahiro Enomura, Hiroyuki Koike, Ran-Ran Zhang, Yasuharu Ueno, Yun-Wen Zheng, Naoto Koike, Shinsuke Aoyama, Yasuhisa Adachi, Hideki Taniguch*(*: Correspondence)
  • Journal Title: Nature Volume: 499 Issue: 7459 Pages: 481-484
  • DOI: 10.1038/nature12271
  • NAID: 40020351046
  • Peer Reviewed
• [Journal Article] The generation of pancreatic β-cell spheroids in a simulated microgravity culture system. (2013)
  • Author(s): Tanaka H
  • Journal Title: Biomaterials Volume: 34 Issue: 23 Pages: 5785-5791
  • DOI: 10.1016/j.biomaterials.2013.04.003
  • Peer Reviewed
• [Journal Article] Human elastic cartilage engineering from cartilage progenitor cells using rotating wall vessel bioreactor. (2012)
  • Author(s): Takanori Takebe, Shinji Kobayashi, Hiroomi Kan, Hiromu Suzuki, Mitsuru Mizuno, Yuichiro Yabuki, Takuro Adegawa, Tomohiko Yoshioka , Junzo Tanaka, Jiro Maegawa, Hideki Taniguchi.
  • Journal Title: Transplantation Proceedings Volume: 44 Issue: 4 Pages: 1158-61
  • DOI: 10.1016/j.transproceed.2012.03.038
  • Peer Reviewed
• [Journal Article] Self-organization of human hepatic organoid by recapitulating organogenesis in vitro (2012)
  • Author(s): Takebe T
  • Journal Title: Transplant Pro Volume: 44(4) Issue: 4 Pages: 1018-1020
  • DOI: 10.1016/j.transproceed.2012.02.007
  • Peer Reviewed
• [Journal Article] Generation of functional human vascular network in vitro (2012)
  • Author(s): Takebe T
  • Journal Title: Transplant Proc Volume: 44(4) Issue: 4 Pages: 1130-1133
  • DOI: 10.1016/j.transproceed.2012.03.039
  • Peer Reviewed
• [Journal Article] Highly efficient generation of definitive endoderm lineage from human induced pluripotent stem cells (2012)
  • Author(s): Sekine K
  • Journal Title: Transplant Proc Volume: 44(4) Issue: 4 Pages: 1127-1129
  • DOI: 10.1016/j.transproceed.2012.03.001
  • Peer Reviewed
• [Journal Article] Regenerative medicine approach as an alternative treatment to islet transplantation. (2012)
  • Author(s): Sekine K
  • Journal Title: Transplant Proc Volume: 44(4) Issue: 4 Pages: 1104-1106
  • DOI: 10.1016/j.transproceed.2012.03.020
  • Peer Reviewed
• [Journal Article] Establishment of Automated Culture System for Murine Induced Pluripotent Stem Cells (2012)
  • Author(s): Koike H
  • Journal Title: BMC Biotechnology Volume: 12 Issue: 1 Pages: 81-81
  • DOI: 10.1186/1472-6750-12-81
  • Peer Reviewed
• [Journal Article] 細胞から臓器へ, 再生医療研究が拓く近未来像 (2012)
  • Author(s): 武部貴則, 谷口英樹
  • Journal Title: Organ Biology Volume: 19(1)
  • NAID: 130004710042
  • Peer Reviewed
• [Presentation] Liver organogenesis. (2015)
  • Author(s): Takebe T
  • Organizer: ASTELLAS FRANCE Annual Meeting of Transplantation Club
  • Place of Presentation: Lille, France
  • Year and Date: 2015-02-06
  • Invited
• [Presentation] Transient vascularization of transplanted human adult-derived progenitors promotes self-organizing cartilage. (2014)
  • Author(s): Takebe T
  • Organizer: Asian Cartilage Repair Society 2nd Annual Congress
  • Place of Presentation: Seoul, Korea
  • Year and Date: 2014-12-07
  • Invited
• [Presentation] 臨床応用へ向けたiPS細胞由来ヒト臓器原基操作技術の開発 (2014)
  • Author(s): 武部貴則
  • Organizer: 第41回日本臓器保存生物医学会学術集会
  • Place of Presentation: 千里ライフサイエンスセンター（大阪府）
  • Year and Date: 2014-11-28
  • Invited
• [Presentation] 器官発生初期プロセスの再現による複雑なヒト臓器の人為的構成 (2014)
  • Author(s): 武部貴則
  • Organizer: 第37回日本分子生物学会年会シンポジウム
  • Place of Presentation: パシフィコ横浜（神奈川県）
  • Year and Date: 2014-11-25 – 2014-11-27
  • Invited
• [Presentation] Realization of iPSC-Organ Bud Transplantation Therapy. (2014)
  • Author(s): Takebe T
  • Organizer: World Alliance forum
  • Place of Presentation: San Francisco, CA, USA
  • Year and Date: 2014-11-06 – 2014-11-07
  • Invited
• [Presentation] Vascularized and functional human liver from an iPSC-derived organ bud transplant. (2014)
  • Author(s): Takebe T
  • Organizer: Advances and Applications of Functional Hepatocytes Symposium
  • Place of Presentation: Shanghai, China
  • Year and Date: 2014-10-29 – 2014-10-30
  • Invited
• [Presentation] Pluripotent stem cells and organ reconstitution for rare diseases (2014)
  • Author(s): Takebe T
  • Organizer: Translational Science of Rare Diseases – From Rare to Care II
  • Place of Presentation: Munich, Germany
  • Year and Date: 2014-10-08 – 2014-10-10
  • Invited
• [Presentation] 多細胞系からなる複雑なヒト臓器の人為的構成 (2014)
  • Author(s): 武部貴則
  • Organizer: 第66回日本生物工学会大会
  • Place of Presentation: 札幌コンベンションセンター（北海道）
  • Year and Date: 2014-09-10
  • Invited
• [Presentation] Vascularized and functional human liver from an iPSC-derived organ bud transplant. (2014)
  • Author(s): Takebe T
  • Organizer: KEY Forum: From Stem Cells to Organs
  • Place of Presentation: 熊本市医師会館（熊本）
  • Year and Date: 2014-09-04
  • Invited
• [Presentation] Vascularized and functional human liver from an iPSC-derived organ bud transplant. (2014)
  • Author(s): 武部貴則
  • Organizer: 47th Annual Meeting for the Japanese Society of Developmental Biologists
  • Place of Presentation: WINC AICHI（愛知県）
  • Year and Date: 2014-05-28
  • Invited
• [Presentation] Realization of human iPSC-derived organ bud transplantation therapy. (2014)
  • Author(s): Takebe T
  • Organizer: The Whole Liver Replacement State-of-the-Science Summit
  • Place of Presentation: Chicago, America
  • Year and Date: 2014-04-29 – 2014-04-30
  • Invited
• [Presentation] Vascularized and functional human liver from an iPSC-derived organ bud transplant. (2014)
  • Author(s): Takebe T
  • Organizer: 7th Pan Pacific Symposium on Stem Cells and Cancer Research (PPSSC)
  • Place of Presentation: Taichung, Taiwan
  • Year and Date: 2014-04-12 – 2014-04-17
  • Invited
• [Presentation] Realization of human iPSC-derived organ bud transplantation therapy. (2014)
  • Author(s): Takanori Takebe
  • Organizer: The Whole Liver Replacement State-of-the-Science Summit
  • Place of Presentation: Chicago, USA
  • Invited
• [Presentation] Vascularized and functional human liver from an iPSC-derived organ bud transplant. (2014)
  • Author(s): Takanori Takebe
  • Organizer: 7th Pan Pacific Symposium on Stem Cells and Cancer Research (PPSSC)
  • Place of Presentation: The Splendor Hotel of Taichung, Taiwan
  • Invited
• [Presentation] 臓器原基創出法を活用した多細胞系からなる複雑な立体組織の人為的構成 (2014)
  • Author(s): 武部貴則、吉川洋史、谷口英樹
  • Organizer: 第13回日本再生医療学会
  • Place of Presentation: 国立京都国際会館（京都府）
  • Invited
• [Presentation] ヒトiPS細胞由来臓器原基移植による機能的なヒト肝臓の創出 (2014)
  • Author(s): 武部貴則、関根圭輔、谷口英樹
  • Organizer: 第13回日本再生医療学会
  • Place of Presentation: 国立京都国際会館（京都府）
  • Invited
• [Presentation] Generation of human liver tissue from an induced pluripotent stem cell-derived organ bud transplant (2013)
  • Author(s): Takanori Takebe
  • Organizer: Symposium 「Current state and future of Regenerative Medicine」, The 90th Annual Meeting of the Physiological Society of Japan
  • Place of Presentation: タワーホール船堀(東京都)(招待講演)
  • Year and Date: 2013-03-29
• [Presentation] Creation of vascularized human organ from induced pluripotent stem cells. (2013)
  • Author(s): Takanori Takebe
  • Organizer: 2nd Regenerative Medicine Seminar
  • Place of Presentation: Seoul, Korea(招待講演)
  • Year and Date: 2013-03-27
• [Presentation] Creation of vascularized and functional human liver from an induced pluripotent stem cell-derived organ bud transplant. (2013)
  • Author(s): Takebe T, Sekine K, Enomura M, Suzuki Y, Koike H, Zhang R, Koike N, Ueno Y, Zheng Y, Taniguchi H
  • Organizer: The 13th Congress of the Asian Society of Transplantation
  • Place of Presentation: 国立京都国際会館（京都府）
• [Presentation] Phenotypic comparison of canine articular and auricular derived chondrocytes for cartilage reconstruction. (2013)
  • Author(s): Mizuno M, Takebe T, Kobayashi S, Lee S, Jo YH, Lee JI, Taniguchi H
  • Organizer: The 13th Congress of the Asian Society of Transplantation
  • Place of Presentation: 国立京都国際会館（京都府）
• [Presentation] Vascularization of primary pancreatic islet towards efficient transplantation. (2013)
  • Author(s): Takahashi Y, Takebe T, Enomura M, Tanaka H, Koike N, Sekine K, Ueno Y, Zheng Y, Taniguchi H
  • Organizer: The 13th Congress of the Asian Society of Transplantation
  • Place of Presentation: 国立京都国際会館（京都府）
• [Presentation] Generation of islet like tissue with human vascular network. (2013)
  • Author(s): Enomura M, Takebe T, Takahashi Y, Tanaka H, Koike N, Sekine K, Taniguchi H
  • Organizer: The 13th Congress of the Asian Society of Transplantation
  • Place of Presentation: 国立京都国際会館（京都府）
• [Presentation] Flow cytometric identification and characterization of endothelial progenitor cells in human umbilical vein endothelial cells. (2013)
  • Author(s): Zheng Y, Aoki K, Tsuchida T, Zhang R, Sekine K, Ogawa M, Takebe T, Yasumura K, Ueno Y, Sakakibara H, Hirahara F, Taniguchi H
  • Organizer: The 13th Congress of the Asian Society of Transplantation
  • Place of Presentation: 国立京都国際会館（京都府）
• [Presentation] Generation of human hepatocyte-like cells from human inducted pluripotent stem cells. (2013)
  • Author(s): Zhang R, Takebe T, Sekine K, Zheng Y, Taniguchi H
  • Organizer: The 13th Congress of the Asian Society of Transplantation
  • Place of Presentation: 国立京都国際会館（京都府）
• [Presentation] Fluorescence visualization of human induced pluripotent stem cells using transcription activator-like (TAL) effector nucleases. (2013)
  • Author(s): Sekine K, Takebe T, Zhang R, Taniguchi H
  • Organizer: The 13th Congress of the Asian Society of Transplantation
  • Place of Presentation: 国立京都国際会館（京都府）
• [Presentation] Effects of natural cartilage extracellular matrix on chondrogenic potential and dedifferentiation for autologous chondrocyte transplantation. (2013)
  • Author(s): Yang W, Lee S, Jo YH, Nemeno JGE, Yeo JE, Nam BM, Kim BY, Takebe T, Lee JI
  • Organizer: The 13th Congress of the Asian Society of Transplantation
  • Place of Presentation: 国立京都国際会館（京都府）
• [Presentation] 3D scaffold-free spheroids fabricated from chondrocytes, synovium-derived, and bone marrow-derived mesenchymal stem cells (BM-MSCs). (2013)
  • Author(s): Nemeno JGE, Jo YH, Lee S, Yang W, Nam BM, Kim BY, Yeo JE, Takebe T, Lee JI
  • Organizer: The 13th Congress of the Asian Society of Transplantation
  • Place of Presentation: 国立京都国際会館（京都府）
• [Presentation] Effect of cryopreservation and cell passaging on cell preparation for autologous chondrocyte transplantation. The 13th Congress of the Asian Society of Transplantation (2013)
  • Author(s): Nam BM, Kim BY, Lee S, Jo YH, Nemeno JGE, Yeo JE, Yang W, Takebe T, Lee JI
  • Organizer: The 13th Congress of the Asian Society of Transplantation
  • Place of Presentation: 国立京都国際会館（京都府）
• [Presentation] Effect of different preservation conditions on cartilage tissue for cell transplantation. (2013)
  • Author(s): Kim BY, Nam BM, Jo YH, Lee S, Jo YH, Nemeno JGE, Yeo JE, Yang W, Takebe T, Lee JI
  • Organizer: The 13th Congress of the Asian Society of Transplantation
  • Place of Presentation: 国立京都国際会館（京都府）
• [Presentation] Assessment of animal pancreatic islets after transportation between Korea and Japan. (2013)
  • Author(s): Lee S, Mizuno M, Jo YH, Yang W, Nemeno JGE, Yeo JE, Nam BM, Kim BY, Takebe T, Taniguchi H, Lee JI
  • Organizer: The 13th Congress of the Asian Society of Transplantation
  • Place of Presentation: 国立京都国際会館（京都府）
• [Presentation] Artificial islet of hybrid cellular spheroids using three different cell lines. (2013)
  • Author(s): Jo YH, Nam BM, Kim BY, Lee S, Nemeno JGE, Yeo JE, Takebe T, Kim YS, Lee JI
  • Organizer: The 13th Congress of the Asian Society of Transplantation
  • Place of Presentation: 国立京都国際会館（京都府）
• [Presentation] Vascularized and functional human liver from an induced pluripotent stem cell-derived organ bud transplant. (2013)
  • Author(s): Takebe T, Sekine K, Enomura M, Suzuki Y, Koike H, Zhang R, Koike N, Ueno Y, Zheng Y, Taniguchi H
  • Organizer: International Society for Stem Cell Research 11th annual meeting
  • Place of Presentation: Boston, USA
• [Presentation] Transient vascularization directs intrinsic cartilage formation from human progenitors. (2013)
  • Author(s): Takebe T, Kobayashi S, Mizuno M, Suzuki H, Chang Yu-Min, Yoshizawa E, Maegawa J, Taniguchi H
  • Organizer: International Society for Stem Cell Research 11th annual meeting
  • Place of Presentation: Boston, USA
• [Presentation] Liver repopulation in a diphtheria toxin-induced liver damage model with human proliferative hepatocytes. (2013)
  • Author(s): Zhang R, Zheng Y, Takebe T, Tsuchida T, Li B, Ueno Y, Taniguchi H
  • Organizer: International Society For Stem Cell Research 11th Annual Meeting
  • Place of Presentation: Boston, USA
• [Presentation] In vitro expansion of self-renewing hepatic progenitor cells by controlling metabolic state (2013)
  • Author(s): Koike H, Takebe T, Ueno Y, Taniguchi H
  • Organizer: International Society for Stem Cell Research 11th annual meeting
  • Place of Presentation: Boston, USA
• [Presentation] Nature Medicine special project (2013)
  • Author(s): Takanori Takebe
  • Organizer: Herrenhausen Symposium on Stem Cells and Regenerative Medicine
  • Place of Presentation: Hannover, Germany
  • Invited
• [Presentation] 如何にして器官発生を模倣するのか?-血管系を有するヒト肝組織創出法の開発- (2013)
  • Author(s): 武部貴則, 関根圭輔, 谷口英樹
  • Organizer: 第12回日本再生医療学会 パネルディスカッション「再生組織に対する血管網付与」
  • Place of Presentation: パシフィコ横浜(神奈川県)(招待講演)
• [Presentation] ヒト耳介由来軟骨前駆細胞を用いた弾性軟骨再構築法の開発 (2013)
  • Author(s): 武部貴則, 小林眞司, 谷口英樹
  • Organizer: 第12回日本再生医療学会 シンポジウム「組織幹細胞の最前線」
  • Place of Presentation: パシフィコ横浜(神奈川県)(招待講演)
• [Presentation] iPS細胞由来肝原基移植に基づく機能的なヒト肝組織の創出 (2012)
  • Author(s): 武部貴則
  • Organizer: 第53回幹細胞治療研究センターフォーラム
  • Place of Presentation: 東京大学医科学研究所(東京都)(招待講演)
  • Year and Date: 2012-11-15
• [Presentation] ヒトiPSを用いたファーマコセロミクス基盤技術の開発 (2012)
  • Author(s): 谷口英樹, 武部貴則, 関根圭輔, 青山晋輔, 安達弥永
  • Organizer: 第127回日本薬理学会関東部会 シンポジウム
  • Place of Presentation: 東京国際フォーラム(東京都)
  • Year and Date: 2012-10-20
• [Presentation] 多能性幹細胞を用いた機能的なヒト臓器の創出 (2012)
  • Author(s): 武部貴則
  • Organizer: ERATO竹内バイオ融合プロジェクト 第1回 奨励会特別研究委員会
  • Place of Presentation: 東京大学生産技術総合研究所(東京都)(招待講演)
  • Year and Date: 2012-07-27
• [Presentation] Creation of vascularized human organ from induced pluripotent stem celis. (2012)
  • Author(s): Takebe T, Sekine K, Enomura M, Suzuki Y, Koike H, Zhang R, Koike N, Ueno Y, Zheng Y, Taniguchi H
  • Organizer: International Society for Stem Cell Research 10^<th> annual meeting
  • Place of Presentation: パシフィコ横浜(神奈川県)
• [Presentation] Generation of islet like structures with human functional vascular networks. (2012)
  • Author(s): Enomura M, Takebe T, Sekine K, Koike N, Taniguchi H, Tanaka H
  • Organizer: International Society for Stem Cell Research 10^<th> annual meeting
  • Place of Presentation: パシフィコ横浜(神奈川県)
• [Presentation] Self-renewal versus differentiation as well as the liver repopulation cap ability of human hepatic stem cells. (2012)
  • Author(s): Zheng y Li B, Zhang R, Kimura M, Tsuchida T, Takebe T, Ueno ySekine K, Taniguchi H
  • Organizer: International Society for Stem Cell Research 10^<th> annual meeting
  • Place of Presentation: パシフィコ横浜(神奈川県)
• [Presentation] Construction of chimeric mice with human immatured hepatocytes. (2012)
  • Author(s): Zhang R, Zheng Y Tsuchida T, Takebe T, Kimura M, Li B, Takiguchi K, Sekine K, Ueno Y, Taniguchi H
  • Organizer: International Society for Stem Cell Research 10^<th> annual meeting.
  • Place of Presentation: パシフィコ横浜(神奈川県)
• [Presentation] Identification of pancreatic stem/progenitor cells expressing PDX1 reside in the CD133 positive pancreatic duct. (2012)
  • Author(s): Sekine K, Ishikawa M, Takebe T, Suzaki A, Kawashimo K, Matsui C, Taniguchi H
  • Organizer: International Society for Stem CeU Research 10^<th> annual meeting
  • Place of Presentation: パシフィコ横浜(神奈川県)
• [Presentation] Reconstitution of Articular(joint) cartilage defects by auricle(ear) derived stem/ progenitor cells. (2012)
  • Author(s): Mizuno M, Kobayashi S, Takebe T, Suzuki H, Murata S, Yabuki Y Hirotomi K, Yasumura K, Maegawa J, Taniguchi H
  • Organizer: International Society for Stem Cell Research 10^<th> annual meeting.
  • Place of Presentation: パシフィコ横浜(神奈川県)
• [Presentation] 多能性幹細胞を用いた機能的なヒト臓器の創出 (2012)
  • Author(s): 武部貴則, 関根圭輔, 江野村允宏, 小池博之, 張冉冉, 三橋優澄, 上野康晴, 鄭允文, 谷英樹
  • Organizer: 第11回日本再生医療学会
  • Place of Presentation: パシフィコ横浜(神奈川県)
• [Presentation] ヒト耳介軟骨膜由来幹/前駆細胞を用いた弾性軟骨再構築法の開発 (2012)
  • Author(s): 武部貴則, 小林眞司, 矢吹雄一郎, 鈴木啓, 水野満, 安村和正, 広富浩一, 鄭允文, 前川二郎, 谷口英樹
  • Organizer: 第11回日本再生医療学会
  • Place of Presentation: パシフィコ横浜(神奈川県)
• [Presentation] 多能性幹細胞を用いた機能的なヒト臓器の創出 (2012)
  • Author(s): 武部貴則, 谷口英樹
  • Organizer: 第39回日本臓器保存生物医学会学術集会 シンポジウム
  • Place of Presentation: コラッセふくしま(福島県)(招待講演)
• [Presentation] iPS細胞からヒト臓器を創る
  • Author(s): 武部貴則
  • Organizer: 横浜市立大学 細胞シグナリング研究会
  • Place of Presentation: 横浜市立大学（神奈川県）
  • Invited
• [Presentation] Vascularized and functional human liver from an iPSC-derived organ bud transplant.
  • Author(s): Takanori Takebe
  • Organizer: THE 12TH US-JAPAN SYMPOSIUM ON DRUG DELIVERY SYSTEMS
  • Place of Presentation: Hawaii, USA
  • Invited
• [Presentation] iPS細胞からヒト臓器を創る
  • Author(s): 武部貴則
  • Organizer: 東京大学医科学研究所 若手シンポジウム
  • Place of Presentation: 東京大学医科学研究所（東京都）
  • Invited
• [Presentation] Vascularized and functional human liver from an iPSC-derived organ bud transplant.
  • Author(s): Takanori Takebe
  • Organizer: Small RNAs to Stem Cells & Epigenetic Reprogramming Asia-2013 Meeting
  • Place of Presentation: University of Tokyo Hongo Campus, Tokyo
  • Invited
• [Presentation] Vascularized and functional human liver from an iPSC-derived organ bud transplant.
  • Author(s): Takanori Takebe
  • Organizer: The 12th Congress International Xenotransplantation Association
  • Place of Presentation: 大阪国際会議場（大阪府）
  • Invited
• [Presentation] iPS細胞からヒト臓器を創る
  • Author(s): 武部貴則
  • Organizer: 第16回日本異種移植研究会
  • Place of Presentation: 大阪国際会議場（大阪府）
  • Invited
• [Presentation] ヒトの臓器を創り出す！
  • Author(s): Takanori Takebe
  • Organizer: Cell aggregation meeting 2013
  • Place of Presentation: ヒルトン福岡シーホーク（福岡県）
  • Invited
• [Presentation] Vascularized and functional human liver from an iPSC-derived organ bud transplant.
  • Author(s): Takanori Takebe
  • Organizer: 4th Regenerative Medicine Seminar sponsored by JSPS and NRF
  • Place of Presentation: Konkuk University, Seoul, Korea
  • Invited
• [Presentation] Vascularized and functional human liver from an iPSC-derived organ bud transplant.
  • Author(s): Takanori Takebe
  • Organizer: The 7th International Cell Therapy Conference
  • Place of Presentation: Seoul, Korea
  • Invited
• [Presentation] Vascularized and functional human liver from an iPSC-derived organ bud transplant.
  • Author(s): Takanori Takebe
  • Organizer: 4th Regenerative Medicine Seminar sponsored by JSPS and NRF
  • Place of Presentation: Inha University, Seoul, Korea
  • Invited
• [Presentation] iPS細胞からヒト臓器を創る
  • Author(s): 武部貴則
  • Organizer: 東京女子医科大学 先端生命医科学研究所 グローバルCOEプログラム セミナー
  • Place of Presentation: 東京女子医科大学（東京都）
  • Invited
• [Presentation] 健やかなミライを実現するための全方位的アプローチ
  • Author(s): 武部貴則
  • Organizer: 倶進会東京支部 セミナー
  • Place of Presentation: 八芳園（東京都）
  • Invited
• [Book] iPS細胞由来器官原基の人為的構成に基づくヒト肝臓の創出 (2014)
  • Author(s): 武部貴則、谷口英樹
  • Total Pages: 4
  • Publisher: 横浜医学
• [Book] 幹細胞と微小環境の相互作用に基づくヒト3次元組織の人為的再構成 (2014)
  • Author(s): 谷口英樹、武部貴則
  • Total Pages: 5
  • Publisher: がん分子標的治療
• [Book] 多能性幹細胞を用いた膵β細胞分化誘導研究の最新動向 (2014)
  • Author(s): 高橋禎暢、武部貴則、谷口英樹
  • Total Pages: 8
  • Publisher: Organ Biology
• [Book] Novel strategies for liver therapy using stem cells. Gut, 2014 3ページ 11.Takebe T, Taniguchi (2014)
  • Author(s): Rashid T, Takebe T, Nakauchi H
  • Total Pages: 3
  • Publisher: Clinical Pharmacology Therapeutics
• [Book] ヒトiPS細胞を活用した再生医療の開発戦略 (2014)
  • Author(s): 谷口英樹、武部貴則
  • Total Pages: 2
  • Publisher: 消化器外科
• [Book] ヒト代謝性臓器の創出に向けた開発戦略 (2014)
  • Author(s): 谷口英樹、武部貴則
  • Total Pages: 9
  • Publisher: 最新医学
• [Book] ヒト臓器の人為的構成に基づく肝細胞の分化誘導 ES・iPS細胞実験スタンダード―再生・創薬・疾患研究のプロトコールと臨床応用の必須知識 (2014)
  • Author(s): 武部貴則、関根圭輔、谷口英樹
  • Total Pages: 10
  • Publisher: 羊土社
• [Book] Human iPSC-Derived Miniature Organs: A Tool for Drug Studies. (2014)
  • Author(s): Takebe T*, Taniguchi H
  • Total Pages: 3
  • Publisher: Clinical Pharmacology & Therapeutics
• [Book] Novel strategies for liver therapy using stem cells. (2014)
  • Author(s): Rashid T, Takebe T, Nakauchi H
  • Total Pages: 3
  • Publisher: Gut
• [Patent(Industrial Property Rights)] 原始腸内胚葉細胞及びその作製方法 (2014)
  • Inventor(s): 武部貴則(40%)、張冉冉(10%)、谷口英樹(50%)
  • Industrial Property Rights Holder: 武部貴則(40%)、張冉冉(10%)、谷口英樹(50%)
  • Industrial Property Rights Type: 特許
  • Filing Date: 2014-12-08
• [Patent(Industrial Property Rights)] 培養方法及び細胞塊 (2014)
  • Inventor(s): 江尻洋子(10%)、谷口英樹(50%)、 武部貴則(40%)
  • Industrial Property Rights Holder: 江尻洋子(10%)、谷口英樹(50%)、 武部貴則(40%)
  • Industrial Property Rights Type: 特許
  • Filing Date: 2014-05-29
• [Patent(Industrial Property Rights)] 自己組織化用細胞集合体の作製方法 (2014)
  • Inventor(s): 武部貴則, 吉川洋史, 谷口英樹
  • Industrial Property Rights Holder: 武部貴則, 吉川洋史, 谷口英樹
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2014-037341
  • Filing Date: 2014-02-27
• [Patent(Industrial Property Rights)] 軟骨細胞の調製方法 (2014)
  • Inventor(s): 武部貴則, 谷口英樹, 小林眞司
  • Industrial Property Rights Holder: 武部貴則, 谷口英樹, 小林眞司
  • Industrial Property Rights Type: 特許
  • Filing Date: 2014-03-21
• [Patent(Industrial Property Rights)] 生物学的組織に血管系を付与する方法 (2013)
  • Inventor(s): 武部貴則, 谷口英樹, 高橋禎暢
  • Industrial Property Rights Holder: 武部貴則, 谷口英樹, 高橋禎暢
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2013-153056
  • Filing Date: 2013-07-23
• [Patent(Industrial Property Rights)] 組織構造体及びその作製方法 (2013)
  • Inventor(s): 江尻洋子, 綾野賢, 福原直人,谷口英樹, 武部貴則
  • Industrial Property Rights Holder: 江尻洋子, 綾野賢, 福原直人,谷口英樹, 武部貴則
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2013-122190
  • Filing Date: 2013-06-10
• [Patent(Industrial Property Rights)] 培養容器及び培養方法 (2013)
  • Inventor(s): 江尻洋子, 綾野賢, 福原直人,谷口英樹, 武部貴則
  • Industrial Property Rights Holder: 江尻洋子, 綾野賢, 福原直人,谷口英樹, 武部貴則
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2013-120915
  • Filing Date: 2013-06-07
• [Patent(Industrial Property Rights)] アミノ酸製剤による細胞増幅法 (2013)
  • Inventor(s): 谷口英樹, 武部貴則, 上野康晴、小池博之
  • Industrial Property Rights Holder: 谷口英樹, 武部貴則, 上野康晴、小池博之
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2013-106289
  • Filing Date: 2013-05-21
• [Patent(Industrial Property Rights)] ヒト組織・臓器の作成方法 (2012)
  • Inventor(s): 谷口英樹, 武部貴則
  • Industrial Property Rights Holder: 谷口英樹, 武部貴則
  • Filing Date: 2012-09-28
  • Overseas
• [Patent(Industrial Property Rights)] 軟骨細胞の調製方法 (2012)
  • Inventor(s): 武部貴則, 谷口英樹, 小林眞司
  • Industrial Property Rights Holder: 武部貴則, 谷口英樹, 小林眞司
  • Industrial Property Number: 2013-058534
  • Filing Date: 2012-03-21