LGR5 splicing variant in intestinal epithelial cells

Research Project

Project/Area Number	24689054
Research Category	Grant-in-Aid for Young Scientists (A)
Allocation Type	Partial Multi-year Fund
Research Field	Digestive surgery
Research Institution	Osaka University
Principal Investigator	NISHIMURA Junichi 大阪大学, 医学(系)研究科(研究院), 助教 (20379209)
Project Period (FY)	2012-04-01 – 2014-03-31
Project Status	Completed (Fiscal Year 2013)
Budget Amount *help	¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000) Fiscal Year 2013: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000) Fiscal Year 2012: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Keywords	LGR5 / スプライシングバリアント / 腸管上皮細胞 / LGR5　splicing　varinat / 腸管上皮幹細胞
Research Abstract	LGR5 splicing variants were expressed in the epithelium of ileum, ascending colon, transverse colon, sigmoid colon, and rectum. HEK293 cells which were overexpressed total LGR5 or splicing variant LGR5 grows slowly in splicing variant overexpressed cells. Wnt signal was activated in splicing variant overexpressed cells. Splicing variant LGR5s were expressed in the upper of CBC using in situ hybridization assay.

Report

(3 results)