| Budget Amount *help |
¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000)
Fiscal Year 2014: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2013: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2012: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
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| Outline of Final Research Achievements |
We found human adipose tissue derived multi-lineage progenitor cells from adipose tissue (ADMPC), In this study, we examined whether some chemical from low molecular compound library could commit human adipose tissue derived multi-lineage progenitor cells (hADMPCs) into cardiac lineage, and we found spermine, known as one of polyamines, can differentiate into cardiac lineage. After that, we examined whether the spermine induced-hADMPCs would differentiate into cardiomyocytes-like cells and improve left ventricular dysfunction in a swine chronic myocardial infarction model.
Spermine, charged with strong cationic charge, prevented the adhesion between cells and the culture dish and it was extremely difficult to make the sheet of drug-induced cardiomyocyte progenitor cells, but we could succeeded in finding a condition to create the sheet in a stable manner.
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